Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans
Background and Objectives: Candida albicans cause a problematic condition in immunocompromised patients that could not be treated quickly due to the resistant behavior of microorganisms. This study aimed to investigate the effect of a novel ionic liquid (IL) as a new drug on C. albicans strains. Ma...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-11-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4183 |
| _version_ | 1797394713194528768 |
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| author | Amir Habibi Mansour Bayat Behinb Omidi Ali Ezabadi Pejman Mortazavi |
| author_facet | Amir Habibi Mansour Bayat Behinb Omidi Ali Ezabadi Pejman Mortazavi |
| author_sort | Amir Habibi |
| collection | DOAJ |
| description |
Background and Objectives: Candida albicans cause a problematic condition in immunocompromised patients that could not be treated quickly due to the resistant behavior of microorganisms. This study aimed to investigate the effect of a novel ionic liquid (IL) as a new drug on C. albicans strains.
Materials and Methods: Seven newly binary ionic liquids mixtures were synthesized, and among them, ([prollinium chloride] [1-methylimidazolium 3-sulfonate] ([pro-HCl][MImS]) was selected and characterized by 1HNMR, 13C NMR, and FT-IR methods. Samples from patients (n=50) with candidiasis were collected and identified through culture media. ERG11 gene overexpression was related to resistance against azole-bearing drugs. The antibiogram, well diffusion assay, MICs, and MFCs tests were operated. PCR and Real-time evaluated the expression of the ERG11 gene, and the rate of cell death was detected using Flow Cytometry.
Results: Our data manifested that this novel IL (Ionic Liquid) can inhibit C. albican's growth, reduce the expression of ERG11 and increase dead cells.
Conclusion: The newly synthesized IL had an inhibiting effect on the growth of the C. albicans strains and may be used as an alternative candidate for novel drug design.
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| first_indexed | 2024-03-09T00:23:44Z |
| format | Article |
| id | doaj.art-d94b21cd4c134b5c9fded10b6a368ebe |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-03-09T00:23:44Z |
| publishDate | 2023-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-d94b21cd4c134b5c9fded10b6a368ebe2023-12-12T04:53:07ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472023-11-0115610.18502/ijm.v15i6.14162Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicansAmir Habibi0Mansour Bayat1Behinb Omidi2Ali Ezabadi3Pejman Mortazavi4Department of Pathobiology, Faculty of Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Pathobiology, Faculty of Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Biology, Central Tehran Branch, Islamic Azad University, Tehran, IranDepartment of Chemistry, Faculty of Science, Central Tehran Branch, Islamic Azad University, Tehran, IranDepartment of Pathobiology, Faculty of Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran Background and Objectives: Candida albicans cause a problematic condition in immunocompromised patients that could not be treated quickly due to the resistant behavior of microorganisms. This study aimed to investigate the effect of a novel ionic liquid (IL) as a new drug on C. albicans strains. Materials and Methods: Seven newly binary ionic liquids mixtures were synthesized, and among them, ([prollinium chloride] [1-methylimidazolium 3-sulfonate] ([pro-HCl][MImS]) was selected and characterized by 1HNMR, 13C NMR, and FT-IR methods. Samples from patients (n=50) with candidiasis were collected and identified through culture media. ERG11 gene overexpression was related to resistance against azole-bearing drugs. The antibiogram, well diffusion assay, MICs, and MFCs tests were operated. PCR and Real-time evaluated the expression of the ERG11 gene, and the rate of cell death was detected using Flow Cytometry. Results: Our data manifested that this novel IL (Ionic Liquid) can inhibit C. albican's growth, reduce the expression of ERG11 and increase dead cells. Conclusion: The newly synthesized IL had an inhibiting effect on the growth of the C. albicans strains and may be used as an alternative candidate for novel drug design. https://ijm.tums.ac.ir/index.php/ijm/article/view/4183Ionic liquid;Imidazole;Proline;ERG11;Candida albicans |
| spellingShingle | Amir Habibi Mansour Bayat Behinb Omidi Ali Ezabadi Pejman Mortazavi Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans Iranian Journal of Microbiology Ionic liquid; Imidazole; Proline; ERG11; Candida albicans |
| title | Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans |
| title_full | Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans |
| title_fullStr | Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans |
| title_full_unstemmed | Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans |
| title_short | Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans |
| title_sort | synthesis of new imidazole based ionic liquids with antifungal activity against candida albicans |
| topic | Ionic liquid; Imidazole; Proline; ERG11; Candida albicans |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4183 |
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