Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages
Bryophytes produce rare and bioactive compounds with a broad range of therapeutic potential, and many species are reported in ethnomedicinal uses. However, only a few studies have investigated their potential as natural anti-inflammatory drug candidate compounds. The present study investigates the a...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/6/1940 |
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| author | Raíssa Volpatto Marques Stefania Enza Sestito Frédéric Bourgaud Sissi Miguel Frédéric Cailotto Pascal Reboul Jean-Yves Jouzeau Sophie Rahuel-Clermont Sandrine Boschi-Muller Henrik Toft Simonsen David Moulin |
| author_facet | Raíssa Volpatto Marques Stefania Enza Sestito Frédéric Bourgaud Sissi Miguel Frédéric Cailotto Pascal Reboul Jean-Yves Jouzeau Sophie Rahuel-Clermont Sandrine Boschi-Muller Henrik Toft Simonsen David Moulin |
| author_sort | Raíssa Volpatto Marques |
| collection | DOAJ |
| description | Bryophytes produce rare and bioactive compounds with a broad range of therapeutic potential, and many species are reported in ethnomedicinal uses. However, only a few studies have investigated their potential as natural anti-inflammatory drug candidate compounds. The present study investigates the anti-inflammatory effects of thirty-two species of bryophytes, including mosses and liverworts, on Raw 264.7 murine macrophages stimulated with lipopolysaccharide (LPS) or recombinant human peroxiredoxin (hPrx1). The 70% ethanol extracts of bryophytes were screened for their potential to reduce the production of nitric oxide (NO), an important pro-inflammatory mediator. Among the analyzed extracts, two moss species significantly inhibited LPS-induced NO production without cytotoxic effects. The bioactive extracts of <i>Dicranum majus</i> and <i>Thuidium delicatulum</i> inhibited NO production in a concentration-dependent manner with IC<sub>50</sub> values of 1.04 and 1.54 µg/mL, respectively. The crude 70% ethanol and ethyl acetate extracts were then partitioned with different solvents in increasing order of polarity (n-hexane, diethyl ether, chloroform, ethyl acetate, and n-butanol). The fractions were screened for their inhibitory effects on NO production stimulated with LPS at 1 ng/mL or 10 ng/mL. The NO production levels were significantly affected by the fractions of decreasing polarity such as n-hexane and diethyl ether ones. Therefore, the potential of these extracts to inhibit the LPS-induced NO pathway suggests their effective properties in attenuating inflammation and could represent a perspective for the development of innovative therapeutic agents. |
| first_indexed | 2024-03-09T13:10:03Z |
| format | Article |
| id | doaj.art-d94e0c4bbfff4d85904afe3381ad78cc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T13:10:03Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d94e0c4bbfff4d85904afe3381ad78cc2023-11-30T21:43:34ZengMDPI AGMolecules1420-30492022-03-01276194010.3390/molecules27061940Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine MacrophagesRaíssa Volpatto Marques0Stefania Enza Sestito1Frédéric Bourgaud2Sissi Miguel3Frédéric Cailotto4Pascal Reboul5Jean-Yves Jouzeau6Sophie Rahuel-Clermont7Sandrine Boschi-Muller8Henrik Toft Simonsen9David Moulin10Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltoft Plads 223, 2800 Kongens Lyngby, DenmarkUMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire IMoPA, Université de Lorraine, 54500 Vandœuvre-lès-Nancy, FrancePlant Advanced Technologies, 19 Avenue de la Forêt de Haye, 54500 Vandœuvre-lès-Nancy, FranceCellengo, 19 Avenue de la Forêt de Haye, 54500 Vandœuvre-lès-Nancy, FranceUMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire IMoPA, Université de Lorraine, 54500 Vandœuvre-lès-Nancy, FranceUMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire IMoPA, Université de Lorraine, 54500 Vandœuvre-lès-Nancy, FranceUMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire IMoPA, Université de Lorraine, 54500 Vandœuvre-lès-Nancy, FranceUMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire IMoPA, Université de Lorraine, 54500 Vandœuvre-lès-Nancy, FranceUMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire IMoPA, Université de Lorraine, 54500 Vandœuvre-lès-Nancy, FranceDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Søltoft Plads 223, 2800 Kongens Lyngby, DenmarkUMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire IMoPA, Université de Lorraine, 54500 Vandœuvre-lès-Nancy, FranceBryophytes produce rare and bioactive compounds with a broad range of therapeutic potential, and many species are reported in ethnomedicinal uses. However, only a few studies have investigated their potential as natural anti-inflammatory drug candidate compounds. The present study investigates the anti-inflammatory effects of thirty-two species of bryophytes, including mosses and liverworts, on Raw 264.7 murine macrophages stimulated with lipopolysaccharide (LPS) or recombinant human peroxiredoxin (hPrx1). The 70% ethanol extracts of bryophytes were screened for their potential to reduce the production of nitric oxide (NO), an important pro-inflammatory mediator. Among the analyzed extracts, two moss species significantly inhibited LPS-induced NO production without cytotoxic effects. The bioactive extracts of <i>Dicranum majus</i> and <i>Thuidium delicatulum</i> inhibited NO production in a concentration-dependent manner with IC<sub>50</sub> values of 1.04 and 1.54 µg/mL, respectively. The crude 70% ethanol and ethyl acetate extracts were then partitioned with different solvents in increasing order of polarity (n-hexane, diethyl ether, chloroform, ethyl acetate, and n-butanol). The fractions were screened for their inhibitory effects on NO production stimulated with LPS at 1 ng/mL or 10 ng/mL. The NO production levels were significantly affected by the fractions of decreasing polarity such as n-hexane and diethyl ether ones. Therefore, the potential of these extracts to inhibit the LPS-induced NO pathway suggests their effective properties in attenuating inflammation and could represent a perspective for the development of innovative therapeutic agents.https://www.mdpi.com/1420-3049/27/6/1940bryophytesmosses<i>Dicranum majus</i><i>Thuidium delicatulum</i>anti-inflammatory activitynitric oxide |
| spellingShingle | Raíssa Volpatto Marques Stefania Enza Sestito Frédéric Bourgaud Sissi Miguel Frédéric Cailotto Pascal Reboul Jean-Yves Jouzeau Sophie Rahuel-Clermont Sandrine Boschi-Muller Henrik Toft Simonsen David Moulin Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages Molecules bryophytes mosses <i>Dicranum majus</i> <i>Thuidium delicatulum</i> anti-inflammatory activity nitric oxide |
| title | Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages |
| title_full | Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages |
| title_fullStr | Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages |
| title_full_unstemmed | Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages |
| title_short | Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages |
| title_sort | anti inflammatory activity of bryophytes extracts in lps stimulated raw264 7 murine macrophages |
| topic | bryophytes mosses <i>Dicranum majus</i> <i>Thuidium delicatulum</i> anti-inflammatory activity nitric oxide |
| url | https://www.mdpi.com/1420-3049/27/6/1940 |
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