A Novel 3D Titanium Surface Produced by Selective Laser Sintering to Counteract <i>Streptococcus oralis</i> Biofilm Formation
The topography of implant surfaces influences the interaction relationship between material and bacteria. The aim of this work was to characterize a novel 3D titanium surface, produced using Selective Laser Sintering (SLS), and to compare the bacterial interaction with machined and double acid etchi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/24/11915 |
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| author | Simonetta D’Ercole Carlo Mangano Luigina Cellini Silvia Di Lodovico Cigdem Atalayin Ozkaya Giovanna Iezzi Adriano Piattelli Morena Petrini |
| author_facet | Simonetta D’Ercole Carlo Mangano Luigina Cellini Silvia Di Lodovico Cigdem Atalayin Ozkaya Giovanna Iezzi Adriano Piattelli Morena Petrini |
| author_sort | Simonetta D’Ercole |
| collection | DOAJ |
| description | The topography of implant surfaces influences the interaction relationship between material and bacteria. The aim of this work was to characterize a novel 3D titanium surface, produced using Selective Laser Sintering (SLS), and to compare the bacterial interaction with machined and double acid etching (DAE) discs. The surface was characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Energy Dispersive X-ray Spectrometry (EDX). The wettability was measured using the sessile method. The microbiological investigation consisted in the cultivation of a bacterial pioneer, <i>Streptococcus oralis</i>, on titanium surfaces, previously covered by human saliva in order to form the acquired pellicle. Then, colony forming units (CFUs), biofilm biomass quantification, analyses of viable and dead cells, and SEM observation were determined after 24 h of <i>S. oralis</i> biofilm formation on the different discs. A significantly higher nano-roughness with respect to the other two groups characterized the novel 3D surface, but the wettability was similar to that of machined samples. The microbiological assays demonstrated that the 3D discs reported significantly lower values of CFUs and biofilm biomass with respect to machined surfaces; however, no significant differences were found with the DAE surfaces. The live/dead staining confirmed the lower percentage of living cells on DAE and 3D surfaces compared with the machined. This novel 3D surface produced by SLS presented a high antiadhesive and antibiofilm activity. |
| first_indexed | 2024-03-10T04:37:05Z |
| format | Article |
| id | doaj.art-d94edd19ed6c47f48a2bf4b3195df328 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T04:37:05Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-d94edd19ed6c47f48a2bf4b3195df3282023-11-23T03:40:22ZengMDPI AGApplied Sciences2076-34172021-12-0111241191510.3390/app112411915A Novel 3D Titanium Surface Produced by Selective Laser Sintering to Counteract <i>Streptococcus oralis</i> Biofilm FormationSimonetta D’Ercole0Carlo Mangano1Luigina Cellini2Silvia Di Lodovico3Cigdem Atalayin Ozkaya4Giovanna Iezzi5Adriano Piattelli6Morena Petrini7Department of Medical, Oral and Biotechnological Science, University G. d’Annunzio of Chieti, Via dei Vestini 31, 66013 Chieti, ItalyIndependent Researcher, Piazza Trento 5, 22015 Como, ItalyDepartment of Pharmacy, University of Chieti, 66013 Chieti, ItalyDepartment of Pharmacy, University of Chieti, 66013 Chieti, ItalyDepartment of Restorative Dentistry, School of Dentistry, Ege University, 35100 Izmir, TurkeyDepartment of Medical, Oral and Biotechnological Science, University G. d’Annunzio of Chieti, Via dei Vestini 31, 66013 Chieti, ItalyFaculty of Medicine and Odontology, Catholic University of San Antonio de Murcia (UCAM), Av. de los Jerónimos, 135, 30107 Murcia, SpainDepartment of Medical, Oral and Biotechnological Science, University G. d’Annunzio of Chieti, Via dei Vestini 31, 66013 Chieti, ItalyThe topography of implant surfaces influences the interaction relationship between material and bacteria. The aim of this work was to characterize a novel 3D titanium surface, produced using Selective Laser Sintering (SLS), and to compare the bacterial interaction with machined and double acid etching (DAE) discs. The surface was characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Energy Dispersive X-ray Spectrometry (EDX). The wettability was measured using the sessile method. The microbiological investigation consisted in the cultivation of a bacterial pioneer, <i>Streptococcus oralis</i>, on titanium surfaces, previously covered by human saliva in order to form the acquired pellicle. Then, colony forming units (CFUs), biofilm biomass quantification, analyses of viable and dead cells, and SEM observation were determined after 24 h of <i>S. oralis</i> biofilm formation on the different discs. A significantly higher nano-roughness with respect to the other two groups characterized the novel 3D surface, but the wettability was similar to that of machined samples. The microbiological assays demonstrated that the 3D discs reported significantly lower values of CFUs and biofilm biomass with respect to machined surfaces; however, no significant differences were found with the DAE surfaces. The live/dead staining confirmed the lower percentage of living cells on DAE and 3D surfaces compared with the machined. This novel 3D surface produced by SLS presented a high antiadhesive and antibiofilm activity.https://www.mdpi.com/2076-3417/11/24/119153D printingselective laser sinteringtitaniumbacteriabiofilm |
| spellingShingle | Simonetta D’Ercole Carlo Mangano Luigina Cellini Silvia Di Lodovico Cigdem Atalayin Ozkaya Giovanna Iezzi Adriano Piattelli Morena Petrini A Novel 3D Titanium Surface Produced by Selective Laser Sintering to Counteract <i>Streptococcus oralis</i> Biofilm Formation Applied Sciences 3D printing selective laser sintering titanium bacteria biofilm |
| title | A Novel 3D Titanium Surface Produced by Selective Laser Sintering to Counteract <i>Streptococcus oralis</i> Biofilm Formation |
| title_full | A Novel 3D Titanium Surface Produced by Selective Laser Sintering to Counteract <i>Streptococcus oralis</i> Biofilm Formation |
| title_fullStr | A Novel 3D Titanium Surface Produced by Selective Laser Sintering to Counteract <i>Streptococcus oralis</i> Biofilm Formation |
| title_full_unstemmed | A Novel 3D Titanium Surface Produced by Selective Laser Sintering to Counteract <i>Streptococcus oralis</i> Biofilm Formation |
| title_short | A Novel 3D Titanium Surface Produced by Selective Laser Sintering to Counteract <i>Streptococcus oralis</i> Biofilm Formation |
| title_sort | novel 3d titanium surface produced by selective laser sintering to counteract i streptococcus oralis i biofilm formation |
| topic | 3D printing selective laser sintering titanium bacteria biofilm |
| url | https://www.mdpi.com/2076-3417/11/24/11915 |
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