Detection of Extended- Spectrum Beta-Lactamase producing Escherichia coli from mesenteric lymph nodes of wild boars (Sus scrofa)
Wild boars (Sus scrofa) are increasing in several European countries, including Italy. In areas with intensive animal farming, like the Italian Emilia-Romagna region, they are likely to be exposed to antimicrobialresistant (AMR) bacteria of livestock origin. In 2017-2018, 108 mesenteric lymph nodes...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2019-02-01
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| Series: | Italian Journal of Food Safety |
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| Online Access: | https://www.pagepressjournals.org/index.php/ijfs/article/view/7707 |
| _version_ | 1811285458312757248 |
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| author | Silvia Bonardi Clotilde Silvia Cabassi Simona Longhi Federico Pia Margherita Corradi Stefano Gilioli Erika Scaltriti |
| author_facet | Silvia Bonardi Clotilde Silvia Cabassi Simona Longhi Federico Pia Margherita Corradi Stefano Gilioli Erika Scaltriti |
| author_sort | Silvia Bonardi |
| collection | DOAJ |
| description | Wild boars (Sus scrofa) are increasing in several European countries, including Italy. In areas with intensive animal farming, like the Italian Emilia-Romagna region, they are likely to be exposed to antimicrobialresistant (AMR) bacteria of livestock origin. In 2017-2018, 108 mesenteric lymph nodes samples were collected from 108 wild boars hunted in Parma province, Emilia-Romagna region, to be tested for ESBL- and carbapenemase-producing Escherichia coli. One isolate (WB-21L) out of 108 (0.9%) was phenotypically confirmed as ESBLproducing E. coli. The strain WB-21L was tested by PCR for the genes blaSHV, blaCTX-M, blaTEM, blaAmpC, blaKPC, blaNDM, blaVIM, blaIMP, blaOXA-48, blaSPM, blaBIC, blaSIM, blaDIM, blaGIM, blaAIM, resulting positive for TEM β-lactamase. Resistance to ampicillin, amoxicillin/clavulanic acid, streptomycin, sulfasomidine, tetracycline and trimethoprim confirmed the multi-resistance nature of the strain WB-21L. Nine E. coli isolates showed resistance to meropenem by the Kirby Bauer test but none of them showed Meropenem MIC values indicative of resistance. In conclusion, the present study shows the presence of ESBL E. coli in wild boars and the possible risk of transfer to game meat handlers and consumers. Future studies are needed to better evaluate the sources of AMR bacteria in wildlife. |
| first_indexed | 2024-04-13T02:44:24Z |
| format | Article |
| id | doaj.art-d960a604f0664fbf95d22541261d6507 |
| institution | Directory Open Access Journal |
| issn | 2239-7132 |
| language | English |
| last_indexed | 2024-04-13T02:44:24Z |
| publishDate | 2019-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Italian Journal of Food Safety |
| spelling | doaj.art-d960a604f0664fbf95d22541261d65072022-12-22T03:06:06ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322019-02-017410.4081/ijfs.2018.7707Detection of Extended- Spectrum Beta-Lactamase producing Escherichia coli from mesenteric lymph nodes of wild boars (Sus scrofa)Silvia Bonardi0Clotilde Silvia Cabassi1Simona Longhi2Federico Pia3Margherita Corradi4Stefano Gilioli5Erika Scaltriti6Department of Veterinary Sciences, University of ParmaDepartment of Veterinary Sciences, University of ParmaDepartment of Veterinary Sciences, University of ParmaDepartment of Veterinary Sciences, University of ParmaManagement Body for Parks and Biodiversity “Emilia Occidentale”, Sala Baganza (PR)Management Body for Parks and Biodiversity “Emilia Occidentale”, Sala Baganza (PR)Experimental Institute for Zooprophylaxis of Lombardy and Emilia-Romagna Regions, Risk Analysis and Genomic Epidemiology Unit, ParmaWild boars (Sus scrofa) are increasing in several European countries, including Italy. In areas with intensive animal farming, like the Italian Emilia-Romagna region, they are likely to be exposed to antimicrobialresistant (AMR) bacteria of livestock origin. In 2017-2018, 108 mesenteric lymph nodes samples were collected from 108 wild boars hunted in Parma province, Emilia-Romagna region, to be tested for ESBL- and carbapenemase-producing Escherichia coli. One isolate (WB-21L) out of 108 (0.9%) was phenotypically confirmed as ESBLproducing E. coli. The strain WB-21L was tested by PCR for the genes blaSHV, blaCTX-M, blaTEM, blaAmpC, blaKPC, blaNDM, blaVIM, blaIMP, blaOXA-48, blaSPM, blaBIC, blaSIM, blaDIM, blaGIM, blaAIM, resulting positive for TEM β-lactamase. Resistance to ampicillin, amoxicillin/clavulanic acid, streptomycin, sulfasomidine, tetracycline and trimethoprim confirmed the multi-resistance nature of the strain WB-21L. Nine E. coli isolates showed resistance to meropenem by the Kirby Bauer test but none of them showed Meropenem MIC values indicative of resistance. In conclusion, the present study shows the presence of ESBL E. coli in wild boars and the possible risk of transfer to game meat handlers and consumers. Future studies are needed to better evaluate the sources of AMR bacteria in wildlife.https://www.pagepressjournals.org/index.php/ijfs/article/view/7707Escherichia coli, Wild boars, ESBL, Antimicrobial resistance. |
| spellingShingle | Silvia Bonardi Clotilde Silvia Cabassi Simona Longhi Federico Pia Margherita Corradi Stefano Gilioli Erika Scaltriti Detection of Extended- Spectrum Beta-Lactamase producing Escherichia coli from mesenteric lymph nodes of wild boars (Sus scrofa) Italian Journal of Food Safety Escherichia coli, Wild boars, ESBL, Antimicrobial resistance. |
| title | Detection of Extended- Spectrum Beta-Lactamase producing Escherichia coli from mesenteric lymph nodes of wild boars (Sus scrofa) |
| title_full | Detection of Extended- Spectrum Beta-Lactamase producing Escherichia coli from mesenteric lymph nodes of wild boars (Sus scrofa) |
| title_fullStr | Detection of Extended- Spectrum Beta-Lactamase producing Escherichia coli from mesenteric lymph nodes of wild boars (Sus scrofa) |
| title_full_unstemmed | Detection of Extended- Spectrum Beta-Lactamase producing Escherichia coli from mesenteric lymph nodes of wild boars (Sus scrofa) |
| title_short | Detection of Extended- Spectrum Beta-Lactamase producing Escherichia coli from mesenteric lymph nodes of wild boars (Sus scrofa) |
| title_sort | detection of extended spectrum beta lactamase producing escherichia coli from mesenteric lymph nodes of wild boars sus scrofa |
| topic | Escherichia coli, Wild boars, ESBL, Antimicrobial resistance. |
| url | https://www.pagepressjournals.org/index.php/ijfs/article/view/7707 |
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