HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway
Colitis-associated cancer (CAC) is a malignant disease of the colon that is caused by recurrent episodes of chronic intestinal inflammation. Huangqi Baizhu decoction (HBD) is a classic prescription comprised of Radix Astragali and Rhizoma Atractylodis, which are usually used to treat digestive condi...
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MDPI AG
2019-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/20/5/1069 |
| _version_ | 1811262874445676544 |
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| author | Song Deng Aiping Wang Xi Chen Qun Du Yanli Wu Gang Chen Wenfeng Guo Yanwu Li |
| author_facet | Song Deng Aiping Wang Xi Chen Qun Du Yanli Wu Gang Chen Wenfeng Guo Yanwu Li |
| author_sort | Song Deng |
| collection | DOAJ |
| description | Colitis-associated cancer (CAC) is a malignant disease of the colon that is caused by recurrent episodes of chronic intestinal inflammation. Huangqi Baizhu decoction (HBD) is a classic prescription comprised of Radix Astragali and Rhizoma Atractylodis, which are usually used to treat digestive conditions, such as peptic ulcers, colitis, or colorectal carcinoma in clinics. HBD is well known for “tonifying qi and spleen” based on the theories of traditional Chinese medicine, and has the preponderant effect of alleviating chronic intestinal mucosa damage associated with disease. However, the underlying mechanism behind this is still unknown. In the current study, we employed the AOM/DSS mouse model to analyze the effects of HBD on the development of inflammation in colonic carcinoma. The in vivo study showed that HBD could significantly reduce the mortality of mice and control the incidence and size of colonic tumors by inhibiting the IL-6/STAT3 signaling pathway. In vitro, Astragaloside and Atractylenolide (CAA), the main components of HBD, inhibited the proliferation of HCT-116 cells as determined by an MTT assay. Furthermore, CAA notably suppressed the protein expression of IL-6R, STAT3, Survivin, and Cyclin D1 induced by IL-6 in HCT-116 and RAW264.7 cells. These results suggested that HBD exhibits anti-inflammatory and anti-proliferative effects, inhibiting the development of CAC in mice. |
| first_indexed | 2024-04-12T19:33:34Z |
| format | Article |
| id | doaj.art-d9623e6660ce4df8859951fede66430f |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T19:33:34Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d9623e6660ce4df8859951fede66430f2022-12-22T03:19:16ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-03-01205106910.3390/ijms20051069ijms20051069HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling PathwaySong Deng0Aiping Wang1Xi Chen2Qun Du3Yanli Wu4Gang Chen5Wenfeng Guo6Yanwu Li7Pi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaPi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaPi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaPi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaPi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaSchool of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, ChinaPi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaPi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaColitis-associated cancer (CAC) is a malignant disease of the colon that is caused by recurrent episodes of chronic intestinal inflammation. Huangqi Baizhu decoction (HBD) is a classic prescription comprised of Radix Astragali and Rhizoma Atractylodis, which are usually used to treat digestive conditions, such as peptic ulcers, colitis, or colorectal carcinoma in clinics. HBD is well known for “tonifying qi and spleen” based on the theories of traditional Chinese medicine, and has the preponderant effect of alleviating chronic intestinal mucosa damage associated with disease. However, the underlying mechanism behind this is still unknown. In the current study, we employed the AOM/DSS mouse model to analyze the effects of HBD on the development of inflammation in colonic carcinoma. The in vivo study showed that HBD could significantly reduce the mortality of mice and control the incidence and size of colonic tumors by inhibiting the IL-6/STAT3 signaling pathway. In vitro, Astragaloside and Atractylenolide (CAA), the main components of HBD, inhibited the proliferation of HCT-116 cells as determined by an MTT assay. Furthermore, CAA notably suppressed the protein expression of IL-6R, STAT3, Survivin, and Cyclin D1 induced by IL-6 in HCT-116 and RAW264.7 cells. These results suggested that HBD exhibits anti-inflammatory and anti-proliferative effects, inhibiting the development of CAC in mice.http://www.mdpi.com/1422-0067/20/5/1069colitis-associated cancerHBDIL-6Rα/STAT3 |
| spellingShingle | Song Deng Aiping Wang Xi Chen Qun Du Yanli Wu Gang Chen Wenfeng Guo Yanwu Li HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway International Journal of Molecular Sciences colitis-associated cancer HBD IL-6Rα/STAT3 |
| title | HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway |
| title_full | HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway |
| title_fullStr | HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway |
| title_full_unstemmed | HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway |
| title_short | HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway |
| title_sort | hbd inhibits the development of colitis associated cancer in mice via the il 6r stat3 signaling pathway |
| topic | colitis-associated cancer HBD IL-6Rα/STAT3 |
| url | http://www.mdpi.com/1422-0067/20/5/1069 |
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