Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution
Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequenc...
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| Format: | Article |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2008-10-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-200804-0004_Glutathione_S-transferases_from_the_larval_gut_of_the_silkworm_Bombyx_mori_cDNA_cloning_gene_structure_expre.php |
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| author | Zhong Zheng GUI Bo Yeon KIM Kwang Sik LEE Ya Dong WEI Xijie GUO Hung Dae SOHN Byung Rae JIN |
| author_facet | Zhong Zheng GUI Bo Yeon KIM Kwang Sik LEE Ya Dong WEI Xijie GUO Hung Dae SOHN Byung Rae JIN |
| author_sort | Zhong Zheng GUI |
| collection | DOAJ |
| description | Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae. |
| first_indexed | 2024-12-17T03:12:19Z |
| format | Article |
| id | doaj.art-d9648eabc01c471787f2132383d7949b |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-17T03:12:19Z |
| publishDate | 2008-10-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-d9648eabc01c471787f2132383d7949b2022-12-21T22:05:48ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292008-10-01105456757410.14411/eje.2008.076eje-200804-0004Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distributionZhong Zheng GUI0Bo Yeon KIM1Kwang Sik LEE2Ya Dong WEI3Xijie GUO4Hung Dae SOHN5Byung Rae JIN6College of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaSericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, ChinaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaTwo glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae.https://www.eje.cz/artkey/eje-200804-0004_Glutathione_S-transferases_from_the_larval_gut_of_the_silkworm_Bombyx_mori_cDNA_cloning_gene_structure_expre.phpbaculovirus expression vectorbombyx moricdna cloningenzymegene structureglutathione s-transferasesilkworm |
| spellingShingle | Zhong Zheng GUI Bo Yeon KIM Kwang Sik LEE Ya Dong WEI Xijie GUO Hung Dae SOHN Byung Rae JIN Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution European Journal of Entomology baculovirus expression vector bombyx mori cdna cloning enzyme gene structure glutathione s-transferase silkworm |
| title | Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution |
| title_full | Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution |
| title_fullStr | Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution |
| title_full_unstemmed | Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution |
| title_short | Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution |
| title_sort | glutathione s transferases from the larval gut of the silkworm bombyx mori cdna cloning gene structure expression and distribution |
| topic | baculovirus expression vector bombyx mori cdna cloning enzyme gene structure glutathione s-transferase silkworm |
| url | https://www.eje.cz/artkey/eje-200804-0004_Glutathione_S-transferases_from_the_larval_gut_of_the_silkworm_Bombyx_mori_cDNA_cloning_gene_structure_expre.php |
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