Investigation of photodamage by femtosecond laser to cells via gold nanorods
Usually, only focused femtosecond (fs) lasers at near-infrared (NIR) range can induce photodamage to transparent cells, making it difficult to treat large amount of cells by such optical methods for photostimulation. In this study, we clarify the mechanism of photodamage to cells that are co-culture...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2017-01-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | http://www.worldscientific.com/doi/pdf/10.1142/S1793545816500346 |
| Summary: | Usually, only focused femtosecond (fs) lasers at near-infrared (NIR) range can induce photodamage to transparent cells, making it difficult to treat large amount of cells by such optical methods for photostimulation. In this study, we clarify the mechanism of photodamage to cells that are co-cultured with gold nanorods (GNRs) by fs laser. The pulse duration and repetition rate of the fs laser play a key role in cell damage suggesting that the heat accumulation contributes to the major part for the cell damage rather than the high peak power which mainly determines the efficiency of multiphoton excitation. We further show that cellular Ca2+ can also be released in this scheme, but the process is more sensitive to peak power. Our results can provide a large-scale GNR-mediated photostimulation for cell signaling modulation. |
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| ISSN: | 1793-5458 1793-7205 |