Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection.
Infectious complications are a common cause of morbidity and mortality in cancer patients undergoing chemotherapy due to increased risk of oral and gastrointestinal candidiasis, candidemia and septicemia. Interactions between C. albicans and endogenous mucosal bacteria are important in understanding...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-04-01
|
| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1007717 |
| _version_ | 1797205872009543680 |
|---|---|
| author | Martinna Bertolini Amit Ranjan Angela Thompson Patricia I Diaz Takanori Sobue Kendra Maas Anna Dongari-Bagtzoglou |
| author_facet | Martinna Bertolini Amit Ranjan Angela Thompson Patricia I Diaz Takanori Sobue Kendra Maas Anna Dongari-Bagtzoglou |
| author_sort | Martinna Bertolini |
| collection | DOAJ |
| description | Infectious complications are a common cause of morbidity and mortality in cancer patients undergoing chemotherapy due to increased risk of oral and gastrointestinal candidiasis, candidemia and septicemia. Interactions between C. albicans and endogenous mucosal bacteria are important in understanding the mechanisms of invasive infection. We published a mouse intravenous chemotherapy model that recapitulates oral and intestinal mucositis, and myelosuppression in patients receiving 5-fluorouracil. We used this model to study the influence of C. albicans on the mucosal bacterial microbiome and compared global community changes in the oral and intestinal mucosa of the same mice. We validated 16S rRNA gene sequencing data by qPCR, in situ hybridization and culture approaches. Mice receiving both 5Fu and C. albicans had an endogenous bacterial overgrowth on the oral but not the small intestinal mucosa. C. albicans infection was associated with loss of mucosal bacterial diversity in both sites with indigenous Stenotrophomonas, Alphaproteobacteria and Enterococcus species dominating the small intestinal, and Enterococcus species dominating the oral mucosa. Both immunosuppression and Candida infection contributed to changes in the oral microbiota. Enterococci isolated from mice with oropharyngeal candidiasis were implicated in degrading the epithelial junction protein E-cadherin and increasing the permeability of the oral epithelial barrier in vitro. Importantly, depletion of these organisms with antibiotics in vivo attenuated oral mucosal E-cadherin degradation and C. albicans invasion without affecting fungal burdens, indicating that bacterial community changes represent overt dysbiosis. Our studies demonstrate a complex interaction between C. albicans, the resident mucosal bacterial microbiota and the host environment in pathogenesis. We shed significant new light on the role of C. albicans in shaping resident bacterial communities and driving mucosal dysbiosis. |
| first_indexed | 2024-04-14T04:23:24Z |
| format | Article |
| id | doaj.art-d96bfa6fa2f74fb987f5bdbce2439a5d |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-24T08:58:01Z |
| publishDate | 2019-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-d96bfa6fa2f74fb987f5bdbce2439a5d2024-04-16T05:31:33ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742019-04-01154e100771710.1371/journal.ppat.1007717Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection.Martinna BertoliniAmit RanjanAngela ThompsonPatricia I DiazTakanori SobueKendra MaasAnna Dongari-BagtzoglouInfectious complications are a common cause of morbidity and mortality in cancer patients undergoing chemotherapy due to increased risk of oral and gastrointestinal candidiasis, candidemia and septicemia. Interactions between C. albicans and endogenous mucosal bacteria are important in understanding the mechanisms of invasive infection. We published a mouse intravenous chemotherapy model that recapitulates oral and intestinal mucositis, and myelosuppression in patients receiving 5-fluorouracil. We used this model to study the influence of C. albicans on the mucosal bacterial microbiome and compared global community changes in the oral and intestinal mucosa of the same mice. We validated 16S rRNA gene sequencing data by qPCR, in situ hybridization and culture approaches. Mice receiving both 5Fu and C. albicans had an endogenous bacterial overgrowth on the oral but not the small intestinal mucosa. C. albicans infection was associated with loss of mucosal bacterial diversity in both sites with indigenous Stenotrophomonas, Alphaproteobacteria and Enterococcus species dominating the small intestinal, and Enterococcus species dominating the oral mucosa. Both immunosuppression and Candida infection contributed to changes in the oral microbiota. Enterococci isolated from mice with oropharyngeal candidiasis were implicated in degrading the epithelial junction protein E-cadherin and increasing the permeability of the oral epithelial barrier in vitro. Importantly, depletion of these organisms with antibiotics in vivo attenuated oral mucosal E-cadherin degradation and C. albicans invasion without affecting fungal burdens, indicating that bacterial community changes represent overt dysbiosis. Our studies demonstrate a complex interaction between C. albicans, the resident mucosal bacterial microbiota and the host environment in pathogenesis. We shed significant new light on the role of C. albicans in shaping resident bacterial communities and driving mucosal dysbiosis.https://doi.org/10.1371/journal.ppat.1007717 |
| spellingShingle | Martinna Bertolini Amit Ranjan Angela Thompson Patricia I Diaz Takanori Sobue Kendra Maas Anna Dongari-Bagtzoglou Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection. PLoS Pathogens |
| title | Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection. |
| title_full | Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection. |
| title_fullStr | Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection. |
| title_full_unstemmed | Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection. |
| title_short | Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection. |
| title_sort | candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection |
| url | https://doi.org/10.1371/journal.ppat.1007717 |
| work_keys_str_mv | AT martinnabertolini candidaalbicansinducesmucosalbacterialdysbiosisthatpromotesinvasiveinfection AT amitranjan candidaalbicansinducesmucosalbacterialdysbiosisthatpromotesinvasiveinfection AT angelathompson candidaalbicansinducesmucosalbacterialdysbiosisthatpromotesinvasiveinfection AT patriciaidiaz candidaalbicansinducesmucosalbacterialdysbiosisthatpromotesinvasiveinfection AT takanorisobue candidaalbicansinducesmucosalbacterialdysbiosisthatpromotesinvasiveinfection AT kendramaas candidaalbicansinducesmucosalbacterialdysbiosisthatpromotesinvasiveinfection AT annadongaribagtzoglou candidaalbicansinducesmucosalbacterialdysbiosisthatpromotesinvasiveinfection |