Molecular-genetic and cytogenetic analyses of cotton chromosome introgression from <i>Gossypium barbadense</i> L. into the genome of <i>G. hirsutum</i> L. in BC<sub>2</sub>F<sub>1</sub> hybrids
Substitution lines of the cotton Gossypium hirsutum L. involving chromosomes of the tetraploid species G. bar ba dense L., G. tomentosum Nutt. ex Seem., and G. mustelinum Miers ex Watt. are a valuable source for breeding, increasing the genetic diversity of G. hirsutum. The substitution of certain G...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2023-12-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/4006 |
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| author | M. F. Sanamyan Sh. U. Bobokhujayev Sh. S. Abdukarimov O. G. Silkova |
| author_facet | M. F. Sanamyan Sh. U. Bobokhujayev Sh. S. Abdukarimov O. G. Silkova |
| author_sort | M. F. Sanamyan |
| collection | DOAJ |
| description | Substitution lines of the cotton Gossypium hirsutum L. involving chromosomes of the tetraploid species G. bar ba dense L., G. tomentosum Nutt. ex Seem., and G. mustelinum Miers ex Watt. are a valuable source for breeding, increasing the genetic diversity of G. hirsutum. The substitution of certain G. hirsutum L. chromosomes with G. barbadense chromosomes affect fibre elongation, fibre yield, fibre strength, and micronaire. To increase the efficiency of creating lines, it is necessary to study the nature of the introgression of alien chromosomes into the G. hirsutum L. genome. As a result of molecular genetic analysis of BC2F1 hybrids obtained from crossing monosomic lines of the cotton G. hirsutum from the cytogenetic collection of Uzbekistan with monosomic backcross hybrids BC1F1 G. hirsutum × G. barbadense on the same chromosomes, genetic differences between the hybrids in the profile of chromosome-specific microsatellite SSR markers were found. The predominant introgression of chromosomes 4, 6 and 12 of the At-subgenome and 22 of the Dt-subgenome of G. barbadense was revealed, while chromosomes 2 and 7 of the At-subgenome and 18 of the Dt- subgenome of G. barbadense were characterized by elimination. Among them, chromosomes 7 of the At- sub genome and 18 of the Dt-subgenome of G. barbadense were eliminated in the first backcross generation. In this work, two lines, CS- B06 and CS-B07, from the American cytogenetic collection with a putative substitution involving chromosomes 6 and 7 of the At-subgenome were analysed. The presence of only polymorphic alleles from the species G. hirsutum and the absence of polymorphic alleles from the species G. barbadense were revealed, which showed the absence of substitution involving these chromosomes. BC2F1 hybrids with monosomy for both G. barbadense and G. hirsutum chromosomes were characterized by regular pairing of chromosomes and high meiotic indexes. However, many hybrids were characterized by a decrease in pollen fertility. Two hybrids with monosomy for chromosome 7 of the At-subgenome of G. hirsutum and chromosome 6 of the At-subgenome of G. barbadense had the greatest reduction in pollen viability (70.09 ± 1.57 and 75.00 ± 1.66 %, respectively). Thus, this work shows a specific feature in the introgression of individual chromosomes of the cotton species G. barbadense into the cotton G. hirsutum genome. |
| first_indexed | 2024-03-07T16:04:27Z |
| format | Article |
| id | doaj.art-d96edb1f448a418295a79626b6cd2580 |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:06:50Z |
| publishDate | 2023-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-d96edb1f448a418295a79626b6cd25802024-04-11T15:31:06ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592023-12-0127895897010.18699/VJGB-23-1101424Molecular-genetic and cytogenetic analyses of cotton chromosome introgression from <i>Gossypium barbadense</i> L. into the genome of <i>G. hirsutum</i> L. in BC<sub>2</sub>F<sub>1</sub> hybridsM. F. Sanamyan0Sh. U. Bobokhujayev1Sh. S. Abdukarimov2O. G. Silkova3National University of Uzbekistan named after Mirzo UlugbekNational University of Uzbekistan named after Mirzo UlugbekCenter of Genomics and Bioinformatics of the Academy of Sciences of the Republic of UzbekistanInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesSubstitution lines of the cotton Gossypium hirsutum L. involving chromosomes of the tetraploid species G. bar ba dense L., G. tomentosum Nutt. ex Seem., and G. mustelinum Miers ex Watt. are a valuable source for breeding, increasing the genetic diversity of G. hirsutum. The substitution of certain G. hirsutum L. chromosomes with G. barbadense chromosomes affect fibre elongation, fibre yield, fibre strength, and micronaire. To increase the efficiency of creating lines, it is necessary to study the nature of the introgression of alien chromosomes into the G. hirsutum L. genome. As a result of molecular genetic analysis of BC2F1 hybrids obtained from crossing monosomic lines of the cotton G. hirsutum from the cytogenetic collection of Uzbekistan with monosomic backcross hybrids BC1F1 G. hirsutum × G. barbadense on the same chromosomes, genetic differences between the hybrids in the profile of chromosome-specific microsatellite SSR markers were found. The predominant introgression of chromosomes 4, 6 and 12 of the At-subgenome and 22 of the Dt-subgenome of G. barbadense was revealed, while chromosomes 2 and 7 of the At-subgenome and 18 of the Dt- subgenome of G. barbadense were characterized by elimination. Among them, chromosomes 7 of the At- sub genome and 18 of the Dt-subgenome of G. barbadense were eliminated in the first backcross generation. In this work, two lines, CS- B06 and CS-B07, from the American cytogenetic collection with a putative substitution involving chromosomes 6 and 7 of the At-subgenome were analysed. The presence of only polymorphic alleles from the species G. hirsutum and the absence of polymorphic alleles from the species G. barbadense were revealed, which showed the absence of substitution involving these chromosomes. BC2F1 hybrids with monosomy for both G. barbadense and G. hirsutum chromosomes were characterized by regular pairing of chromosomes and high meiotic indexes. However, many hybrids were characterized by a decrease in pollen fertility. Two hybrids with monosomy for chromosome 7 of the At-subgenome of G. hirsutum and chromosome 6 of the At-subgenome of G. barbadense had the greatest reduction in pollen viability (70.09 ± 1.57 and 75.00 ± 1.66 %, respectively). Thus, this work shows a specific feature in the introgression of individual chromosomes of the cotton species G. barbadense into the cotton G. hirsutum genome.https://vavilov.elpub.ru/jour/article/view/4006cottongossypium hirsutumg. barbadensemonosomic lineschromosome-substituted hybridsmolecular genetic analysis |
| spellingShingle | M. F. Sanamyan Sh. U. Bobokhujayev Sh. S. Abdukarimov O. G. Silkova Molecular-genetic and cytogenetic analyses of cotton chromosome introgression from <i>Gossypium barbadense</i> L. into the genome of <i>G. hirsutum</i> L. in BC<sub>2</sub>F<sub>1</sub> hybrids Вавиловский журнал генетики и селекции cotton gossypium hirsutum g. barbadense monosomic lines chromosome-substituted hybrids molecular genetic analysis |
| title | Molecular-genetic and cytogenetic analyses of cotton chromosome introgression from <i>Gossypium barbadense</i> L. into the genome of <i>G. hirsutum</i> L. in BC<sub>2</sub>F<sub>1</sub> hybrids |
| title_full | Molecular-genetic and cytogenetic analyses of cotton chromosome introgression from <i>Gossypium barbadense</i> L. into the genome of <i>G. hirsutum</i> L. in BC<sub>2</sub>F<sub>1</sub> hybrids |
| title_fullStr | Molecular-genetic and cytogenetic analyses of cotton chromosome introgression from <i>Gossypium barbadense</i> L. into the genome of <i>G. hirsutum</i> L. in BC<sub>2</sub>F<sub>1</sub> hybrids |
| title_full_unstemmed | Molecular-genetic and cytogenetic analyses of cotton chromosome introgression from <i>Gossypium barbadense</i> L. into the genome of <i>G. hirsutum</i> L. in BC<sub>2</sub>F<sub>1</sub> hybrids |
| title_short | Molecular-genetic and cytogenetic analyses of cotton chromosome introgression from <i>Gossypium barbadense</i> L. into the genome of <i>G. hirsutum</i> L. in BC<sub>2</sub>F<sub>1</sub> hybrids |
| title_sort | molecular genetic and cytogenetic analyses of cotton chromosome introgression from i gossypium barbadense i l into the genome of i g hirsutum i l in bc sub 2 sub f sub 1 sub hybrids |
| topic | cotton gossypium hirsutum g. barbadense monosomic lines chromosome-substituted hybrids molecular genetic analysis |
| url | https://vavilov.elpub.ru/jour/article/view/4006 |
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