Isolation and characterization of Β-sitosterol from methanol extracts of the stem bark of large- leaved rock fig (<i>Ficus abutilifolia</i> Miq)

This work was aimed at isolating and characterizing some bioactive constituent(s) from the stem bark of Ficus abutilifolia. The phytochemical analysis of the crude methanol extract indicates the presence of flavonoids, alkaloids, cardiac glycosides, saponnins, tannin, steroids and triterpenes. The...

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Bibliographic Details
Main Authors: M.O. Aliba, I.G. Ndukwe, H Ibrahim
Format: Article
Language:English
Published: Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) 2018-11-01
Series:Journal of Applied Sciences and Environmental Management
Subjects:
Online Access:https://www.ajol.info/index.php/jasem/article/view/180257
Description
Summary:This work was aimed at isolating and characterizing some bioactive constituent(s) from the stem bark of Ficus abutilifolia. The phytochemical analysis of the crude methanol extract indicates the presence of flavonoids, alkaloids, cardiac glycosides, saponnins, tannin, steroids and triterpenes. The extract was subjected to column chromatography which resulted in the isolation and purification of a white needle-like crystalline substance with a melting point of 134 – 1360C, the 13CNMR shows recognizable peak at 71.98 ppm assignable to the beta hydroxyl group and the 1HNMR spectrum which shows an olefinic proton at δ 5.37 was identified as β-sitosterol. Identification of the compound was purely by spectral evidence. Keywords:  Ficus abutilifolia, methanol extracts, phytochemical analysis, β-sitosterol
ISSN:2659-1502
2659-1499