Effects of Taro (<i>Colocasia esculenta</i>) Water-Soluble Non-Starch Polysaccharide, <i>Lactobacillus</i> <i>acidophilus</i>, <i>Bifidobacterium breve</i>, <i>Bifidobacterium infantis</i>, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production
In the past decades, the regulation of pro-inflammatory cytokine production, including interleukin-8 (IL-8), has been the goal of many targeted therapeutic interventions for Necrotising enterocolitis (NEC), a gastrointestinal disease commonly associated with a very low birth weight in preterm infant...
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MDPI AG
2022-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/10/2128 |
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| author | Mylene Anwar Sonya Mros Michelle McConnell Alaa El-Din A. Bekhit |
| author_facet | Mylene Anwar Sonya Mros Michelle McConnell Alaa El-Din A. Bekhit |
| author_sort | Mylene Anwar |
| collection | DOAJ |
| description | In the past decades, the regulation of pro-inflammatory cytokine production, including interleukin-8 (IL-8), has been the goal of many targeted therapeutic interventions for Necrotising enterocolitis (NEC), a gastrointestinal disease commonly associated with a very low birth weight in preterm infants. In this study, the ability to regulate the production of IL-8 of the water-soluble non-starch polysaccharide (WS-NSP) from taro corm (Tc-WS-NSP) extracted using a conventional (CE) or improved conventional (ICE) extraction method, of the probiotics <i>Lactobacillus acidophilus</i>, <i>Bifidobacterium breve</i>, and <i>Bifidobacterium infantis</i>, and their synbiotic mixtures were evaluated. The TNF-α stimulated HT-29 cells were incubated with undigested or digested Tc-WS-NSPs (CE or ICE), probiotics, and their synbiotic mixtures with <i>Klebsiella oxytoca</i>, an NEC-positive-associated pathogen. Overall, the synbiotic mixtures of digested Tc-WS-NSP-ICE and high bacterial concentrations of <i>L. acidophilus</i> (5.57 × 10<sup>9</sup>), <i>B. breve</i> (2.7 × 10<sup>8</sup> CFU/mL), and <i>B. infantis</i> (1.53 × 10<sup>8</sup>) demonstrated higher (42.0%, 45.0%, 43.1%, respectively) ability to downregulate IL-8 compared to the sole use of Tc-WS-NSPs (24.5%), or the probiotics <i>L. acidophilus</i> (32.3%), <i>B. breve</i> (37.8%), or <i>B. infantis</i> (33.1%). The ability demonstrated by the Tc-WS-NSPs, the probiotics, and their synbiotics mixtures to downregulate IL-8 production in the presence of an NEC-positive-associated pathogen may be useful in the development of novel prophylactic agents against NEC. |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:13:48Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-d971b9e590fb4ac2a9a7982d9d6ee5da2023-11-23T12:30:31ZengMDPI AGNutrients2072-66432022-05-011410212810.3390/nu14102128Effects of Taro (<i>Colocasia esculenta</i>) Water-Soluble Non-Starch Polysaccharide, <i>Lactobacillus</i> <i>acidophilus</i>, <i>Bifidobacterium breve</i>, <i>Bifidobacterium infantis</i>, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 ProductionMylene Anwar0Sonya Mros1Michelle McConnell2Alaa El-Din A. Bekhit3Department of Food Science, University of Otago, P.O. Box 56, Dunedin 9054, New ZealandDepartment of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin 9054, New ZealandDepartment of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin 9054, New ZealandDepartment of Food Science, University of Otago, P.O. Box 56, Dunedin 9054, New ZealandIn the past decades, the regulation of pro-inflammatory cytokine production, including interleukin-8 (IL-8), has been the goal of many targeted therapeutic interventions for Necrotising enterocolitis (NEC), a gastrointestinal disease commonly associated with a very low birth weight in preterm infants. In this study, the ability to regulate the production of IL-8 of the water-soluble non-starch polysaccharide (WS-NSP) from taro corm (Tc-WS-NSP) extracted using a conventional (CE) or improved conventional (ICE) extraction method, of the probiotics <i>Lactobacillus acidophilus</i>, <i>Bifidobacterium breve</i>, and <i>Bifidobacterium infantis</i>, and their synbiotic mixtures were evaluated. The TNF-α stimulated HT-29 cells were incubated with undigested or digested Tc-WS-NSPs (CE or ICE), probiotics, and their synbiotic mixtures with <i>Klebsiella oxytoca</i>, an NEC-positive-associated pathogen. Overall, the synbiotic mixtures of digested Tc-WS-NSP-ICE and high bacterial concentrations of <i>L. acidophilus</i> (5.57 × 10<sup>9</sup>), <i>B. breve</i> (2.7 × 10<sup>8</sup> CFU/mL), and <i>B. infantis</i> (1.53 × 10<sup>8</sup>) demonstrated higher (42.0%, 45.0%, 43.1%, respectively) ability to downregulate IL-8 compared to the sole use of Tc-WS-NSPs (24.5%), or the probiotics <i>L. acidophilus</i> (32.3%), <i>B. breve</i> (37.8%), or <i>B. infantis</i> (33.1%). The ability demonstrated by the Tc-WS-NSPs, the probiotics, and their synbiotics mixtures to downregulate IL-8 production in the presence of an NEC-positive-associated pathogen may be useful in the development of novel prophylactic agents against NEC.https://www.mdpi.com/2072-6643/14/10/2128taro (<i>Colocasia esculenta</i>)water-soluble non-starch polysaccharideprobioticssynbioticinterleukin 8necrotising enterocolitis |
| spellingShingle | Mylene Anwar Sonya Mros Michelle McConnell Alaa El-Din A. Bekhit Effects of Taro (<i>Colocasia esculenta</i>) Water-Soluble Non-Starch Polysaccharide, <i>Lactobacillus</i> <i>acidophilus</i>, <i>Bifidobacterium breve</i>, <i>Bifidobacterium infantis</i>, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production Nutrients taro (<i>Colocasia esculenta</i>) water-soluble non-starch polysaccharide probiotics synbiotic interleukin 8 necrotising enterocolitis |
| title | Effects of Taro (<i>Colocasia esculenta</i>) Water-Soluble Non-Starch Polysaccharide, <i>Lactobacillus</i> <i>acidophilus</i>, <i>Bifidobacterium breve</i>, <i>Bifidobacterium infantis</i>, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production |
| title_full | Effects of Taro (<i>Colocasia esculenta</i>) Water-Soluble Non-Starch Polysaccharide, <i>Lactobacillus</i> <i>acidophilus</i>, <i>Bifidobacterium breve</i>, <i>Bifidobacterium infantis</i>, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production |
| title_fullStr | Effects of Taro (<i>Colocasia esculenta</i>) Water-Soluble Non-Starch Polysaccharide, <i>Lactobacillus</i> <i>acidophilus</i>, <i>Bifidobacterium breve</i>, <i>Bifidobacterium infantis</i>, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production |
| title_full_unstemmed | Effects of Taro (<i>Colocasia esculenta</i>) Water-Soluble Non-Starch Polysaccharide, <i>Lactobacillus</i> <i>acidophilus</i>, <i>Bifidobacterium breve</i>, <i>Bifidobacterium infantis</i>, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production |
| title_short | Effects of Taro (<i>Colocasia esculenta</i>) Water-Soluble Non-Starch Polysaccharide, <i>Lactobacillus</i> <i>acidophilus</i>, <i>Bifidobacterium breve</i>, <i>Bifidobacterium infantis</i>, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production |
| title_sort | effects of taro i colocasia esculenta i water soluble non starch polysaccharide i lactobacillus i i acidophilus i i bifidobacterium breve i i bifidobacterium infantis i and their synbiotic mixtures on pro inflammatory cytokine interleukin 8 production |
| topic | taro (<i>Colocasia esculenta</i>) water-soluble non-starch polysaccharide probiotics synbiotic interleukin 8 necrotising enterocolitis |
| url | https://www.mdpi.com/2072-6643/14/10/2128 |
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