Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues
Abstract Background Intratumoral microbial communities have been recently discovered to exist in a variety of cancers and have been found to be intricately involved in tumour progression. Therefore, investigating the profiles and functions of intratumoral microbial distribution in hepatocellular car...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-02-01
|
| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12967-023-03938-6 |
| _version_ | 1811171593362079744 |
|---|---|
| author | Yuting He Qiyao Zhang Xiao Yu Shuijun Zhang Wenzhi Guo |
| author_facet | Yuting He Qiyao Zhang Xiao Yu Shuijun Zhang Wenzhi Guo |
| author_sort | Yuting He |
| collection | DOAJ |
| description | Abstract Background Intratumoral microbial communities have been recently discovered to exist in a variety of cancers and have been found to be intricately involved in tumour progression. Therefore, investigating the profiles and functions of intratumoral microbial distribution in hepatocellular carcinoma (HCC) is imperative. Methods To verify the presence of microorganisms in HCC, we performed fluorescence in situ hybridization (FISH) using HCC tissues and conducted MiSeq using 99 HCC and paracancerous tissues to identify the key microorganisms and changes in metabolic pathways affecting HCC progression through a variety of bioinformatics methods. Results Microbial diversity was significantly higher in HCC tissues than in adjacent tissues. The abundances of microorganisms such as Enterobacteriaceae, Fusobacterium and Neisseria were significantly increased in HCC tissues, while the abundances of certain antitumour bacteria such as Pseudomonas were decreased. Processes such as fatty acid and lipid synthesis were significantly enhanced in the microbiota in HCC tissues, which may be a key factor through which intratumoral microbes influence tumour progression. There were considerable differences in the microbes and their functions within tumour tissue collected from patients with different clinical features. Conclusion We comprehensively evaluated the intratumoral microbial atlas of HCC tissue and preliminarily explored the mechanism of the effects of the microbial community involving changes in lipid metabolism and effects on HCC progression, which lays the foundation for further research in this field. |
| first_indexed | 2024-04-10T17:16:39Z |
| format | Article |
| id | doaj.art-d97418ad5bec46e681b87ba8db633c26 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-10T17:16:39Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-d97418ad5bec46e681b87ba8db633c262023-02-05T12:22:29ZengBMCJournal of Translational Medicine1479-58762023-02-0121111210.1186/s12967-023-03938-6Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissuesYuting He0Qiyao Zhang1Xiao Yu2Shuijun Zhang3Wenzhi Guo4Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou UniversityAbstract Background Intratumoral microbial communities have been recently discovered to exist in a variety of cancers and have been found to be intricately involved in tumour progression. Therefore, investigating the profiles and functions of intratumoral microbial distribution in hepatocellular carcinoma (HCC) is imperative. Methods To verify the presence of microorganisms in HCC, we performed fluorescence in situ hybridization (FISH) using HCC tissues and conducted MiSeq using 99 HCC and paracancerous tissues to identify the key microorganisms and changes in metabolic pathways affecting HCC progression through a variety of bioinformatics methods. Results Microbial diversity was significantly higher in HCC tissues than in adjacent tissues. The abundances of microorganisms such as Enterobacteriaceae, Fusobacterium and Neisseria were significantly increased in HCC tissues, while the abundances of certain antitumour bacteria such as Pseudomonas were decreased. Processes such as fatty acid and lipid synthesis were significantly enhanced in the microbiota in HCC tissues, which may be a key factor through which intratumoral microbes influence tumour progression. There were considerable differences in the microbes and their functions within tumour tissue collected from patients with different clinical features. Conclusion We comprehensively evaluated the intratumoral microbial atlas of HCC tissue and preliminarily explored the mechanism of the effects of the microbial community involving changes in lipid metabolism and effects on HCC progression, which lays the foundation for further research in this field.https://doi.org/10.1186/s12967-023-03938-6Hepatocellular carcinomaIntratumoral microbiotaMetabolic pathwayFatty acid and lipid synthesisMiSeq |
| spellingShingle | Yuting He Qiyao Zhang Xiao Yu Shuijun Zhang Wenzhi Guo Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues Journal of Translational Medicine Hepatocellular carcinoma Intratumoral microbiota Metabolic pathway Fatty acid and lipid synthesis MiSeq |
| title | Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues |
| title_full | Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues |
| title_fullStr | Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues |
| title_full_unstemmed | Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues |
| title_short | Overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues |
| title_sort | overview of microbial profiles in human hepatocellular carcinoma and adjacent nontumor tissues |
| topic | Hepatocellular carcinoma Intratumoral microbiota Metabolic pathway Fatty acid and lipid synthesis MiSeq |
| url | https://doi.org/10.1186/s12967-023-03938-6 |
| work_keys_str_mv | AT yutinghe overviewofmicrobialprofilesinhumanhepatocellularcarcinomaandadjacentnontumortissues AT qiyaozhang overviewofmicrobialprofilesinhumanhepatocellularcarcinomaandadjacentnontumortissues AT xiaoyu overviewofmicrobialprofilesinhumanhepatocellularcarcinomaandadjacentnontumortissues AT shuijunzhang overviewofmicrobialprofilesinhumanhepatocellularcarcinomaandadjacentnontumortissues AT wenzhiguo overviewofmicrobialprofilesinhumanhepatocellularcarcinomaandadjacentnontumortissues |