Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India
The present study was aimed to evaluate the antimicrobial and antioxidant potential of ethanolic extract and its different solvent fractions (chloroform, ethyl acetate, n-butanol and aqueous fraction) of bark and leaves of Terminalia arjuna. The antimicrobial activity was determined by disc diffusio...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-01-01
|
| Series: | Journal of Traditional and Complementary Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411017300421 |
| _version_ | 1818549963858116608 |
|---|---|
| author | Vikas Kumar Nitin Sharma Anuradha Sourirajan Prem Kumar Khosla Kamal Dev |
| author_facet | Vikas Kumar Nitin Sharma Anuradha Sourirajan Prem Kumar Khosla Kamal Dev |
| author_sort | Vikas Kumar |
| collection | DOAJ |
| description | The present study was aimed to evaluate the antimicrobial and antioxidant potential of ethanolic extract and its different solvent fractions (chloroform, ethyl acetate, n-butanol and aqueous fraction) of bark and leaves of Terminalia arjuna. The antimicrobial activity was determined by disc diffusion and minimum inhibitory concentration (MIC) methods against six bacterial stains. The antioxidant activity was evaluated by using DPPH, FRAP and Nitric oxide (NO) scavenging assay. The total phenolics and flavonoid content were found to be higher in n-butanolic fraction of bark (294.6 ± 8.1 mg/g GAE and 168.6 ± 12.3 mg/g RE, respectively) and leaves (203.7 ± 7.0 mg/g GAE and 144.8 ± 11.1 mg/g RE, respectively). The maximum antimicrobial activity was shown by n-butanolic fraction of bark and leaves. The zone of inhibition of 15.0 ± 0.7 mm, 15.5 ± 0.7 mm, 15.0 ± 1.5 mm, 15.5 ± 0.7 mm, 15.0 ± 0.7 mm, 15.0 ± 0.7 mm was observed against Bacillus subtilis, Staphylococcus aureus, Eschericia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella typhi respectively. In case of leaves extract, zone of inhibition of 13.5 ± 0.7 mm, 16.5 ± 0.7 mm, 14.0 ± 0.5 mm, 15.0 ± 0.5 mm, 13.5 ± 0.7 mm, 14.0 ± 0.7 mm was observed against B. subtilis, S. aureus, E. coli, K. pneumoniae, P. aeruginosa, S. typhi, respectively. The n-butanol fraction of bark [IC50-4.1 μg/ml (DPPH), 21.0 μM (FRAP), 3.3 μg/ml (NO)] and leaves [IC50-4.8 μg/ml (DPPH), 28.9 μM (FRAP), 3.2 μg/ml (NO)] showed more antioxidant potential as compared to that of crude ethanolic extract, ethyl acetate fraction, chloroform fraction, aqueous fraction and even ascorbic acid. These results clearly indicated comparative antioxidant potential and antimicrobial activity in extracts of bark and leaves of T. arjuna. |
| first_indexed | 2024-12-12T08:40:08Z |
| format | Article |
| id | doaj.art-d974b2cbb74e4bf1a6a732245003bbd9 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-12-12T08:40:08Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-d974b2cbb74e4bf1a6a732245003bbd92022-12-22T00:30:49ZengElsevierJournal of Traditional and Complementary Medicine2225-41102018-01-018110010610.1016/j.jtcme.2017.04.002Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, IndiaVikas Kumar0Nitin Sharma1Anuradha Sourirajan2Prem Kumar Khosla3Kamal Dev4Faculty of Applied Sciences and Biotechnology, Shoolini University, Post Box No. 9, Head Post Office, Solan, H.P., IndiaFaculty of Applied Sciences and Biotechnology, Shoolini University, Post Box No. 9, Head Post Office, Solan, H.P., IndiaFaculty of Applied Sciences and Biotechnology, Shoolini University, Post Box No. 9, Head Post Office, Solan, H.P., IndiaFaculty of Biological Sciences and Environmnetal Sciences, Shoolini University, Post Box No. 9, Head Post Office, Solan H.P., IndiaFaculty of Applied Sciences and Biotechnology, Shoolini University, Post Box No. 9, Head Post Office, Solan, H.P., IndiaThe present study was aimed to evaluate the antimicrobial and antioxidant potential of ethanolic extract and its different solvent fractions (chloroform, ethyl acetate, n-butanol and aqueous fraction) of bark and leaves of Terminalia arjuna. The antimicrobial activity was determined by disc diffusion and minimum inhibitory concentration (MIC) methods against six bacterial stains. The antioxidant activity was evaluated by using DPPH, FRAP and Nitric oxide (NO) scavenging assay. The total phenolics and flavonoid content were found to be higher in n-butanolic fraction of bark (294.6 ± 8.1 mg/g GAE and 168.6 ± 12.3 mg/g RE, respectively) and leaves (203.7 ± 7.0 mg/g GAE and 144.8 ± 11.1 mg/g RE, respectively). The maximum antimicrobial activity was shown by n-butanolic fraction of bark and leaves. The zone of inhibition of 15.0 ± 0.7 mm, 15.5 ± 0.7 mm, 15.0 ± 1.5 mm, 15.5 ± 0.7 mm, 15.0 ± 0.7 mm, 15.0 ± 0.7 mm was observed against Bacillus subtilis, Staphylococcus aureus, Eschericia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella typhi respectively. In case of leaves extract, zone of inhibition of 13.5 ± 0.7 mm, 16.5 ± 0.7 mm, 14.0 ± 0.5 mm, 15.0 ± 0.5 mm, 13.5 ± 0.7 mm, 14.0 ± 0.7 mm was observed against B. subtilis, S. aureus, E. coli, K. pneumoniae, P. aeruginosa, S. typhi, respectively. The n-butanol fraction of bark [IC50-4.1 μg/ml (DPPH), 21.0 μM (FRAP), 3.3 μg/ml (NO)] and leaves [IC50-4.8 μg/ml (DPPH), 28.9 μM (FRAP), 3.2 μg/ml (NO)] showed more antioxidant potential as compared to that of crude ethanolic extract, ethyl acetate fraction, chloroform fraction, aqueous fraction and even ascorbic acid. These results clearly indicated comparative antioxidant potential and antimicrobial activity in extracts of bark and leaves of T. arjuna.http://www.sciencedirect.com/science/article/pii/S2225411017300421Terminalia arjunaAntimicrobialsAntioxidantsIC50Medicinal trees |
| spellingShingle | Vikas Kumar Nitin Sharma Anuradha Sourirajan Prem Kumar Khosla Kamal Dev Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India Journal of Traditional and Complementary Medicine Terminalia arjuna Antimicrobials Antioxidants IC50 Medicinal trees |
| title | Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India |
| title_full | Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India |
| title_fullStr | Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India |
| title_full_unstemmed | Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India |
| title_short | Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India |
| title_sort | comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of terminalia arjuna from north western himalayas india |
| topic | Terminalia arjuna Antimicrobials Antioxidants IC50 Medicinal trees |
| url | http://www.sciencedirect.com/science/article/pii/S2225411017300421 |
| work_keys_str_mv | AT vikaskumar comparativeevaluationofantimicrobialandantioxidantpotentialofethanolicextractanditsfractionsofbarkandleavesofterminaliaarjunafromnorthwesternhimalayasindia AT nitinsharma comparativeevaluationofantimicrobialandantioxidantpotentialofethanolicextractanditsfractionsofbarkandleavesofterminaliaarjunafromnorthwesternhimalayasindia AT anuradhasourirajan comparativeevaluationofantimicrobialandantioxidantpotentialofethanolicextractanditsfractionsofbarkandleavesofterminaliaarjunafromnorthwesternhimalayasindia AT premkumarkhosla comparativeevaluationofantimicrobialandantioxidantpotentialofethanolicextractanditsfractionsofbarkandleavesofterminaliaarjunafromnorthwesternhimalayasindia AT kamaldev comparativeevaluationofantimicrobialandantioxidantpotentialofethanolicextractanditsfractionsofbarkandleavesofterminaliaarjunafromnorthwesternhimalayasindia |