A robust yeast chassis: comprehensive characterization of a fast-growing Saccharomyces cerevisiae
ABSTRACTRobust chassis are critical to facilitate advances in synthetic biology. This study describes a comprehensive characterization of a new yeast isolate Saccharomyces cerevisiae XP that grows faster than commonly used research and industrial S. cerevisiae strains. The genomic, transcriptomic, a...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2024-02-01
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| Series: | mBio |
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| Online Access: | https://journals.asm.org/doi/10.1128/mbio.03196-23 |
| _version_ | 1797309368200331264 |
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| author | Yangdanyu Long Xiao Han Xuanlin Meng Ping Xu Fei Tao |
| author_facet | Yangdanyu Long Xiao Han Xuanlin Meng Ping Xu Fei Tao |
| author_sort | Yangdanyu Long |
| collection | DOAJ |
| description | ABSTRACTRobust chassis are critical to facilitate advances in synthetic biology. This study describes a comprehensive characterization of a new yeast isolate Saccharomyces cerevisiae XP that grows faster than commonly used research and industrial S. cerevisiae strains. The genomic, transcriptomic, and metabolomic analyses suggest that the fast growth rate is, in part, due to the efficient electron transport chain and key growth factor synthesis. A toolbox for genetic manipulation of the yeast was developed; we used it to construct l-lactic acid producers for high lactate production. The development of genetically malleable yeast strains that grow faster than currently used strains may significantly enhance the uses of S. cerevisiae in biotechnology.IMPORTANCEYeast is known as an outstanding starting strain for constructing microbial cell factories. However, its growth rate restricts its application. A yeast strain XP, which grows fast in high concentrations of sugar and acidic environments, is revealed to demonstrate the potential in industrial applications. A toolbox was also built for its genetic manipulation including gene insertion, deletion, and ploidy transformation. The knowledge of its metabolism, which could guide the designing of genetic experiments, was generated with multi-omics analyses. This novel strain along with its toolbox was then tested by constructing an l-lactic acid efficient producer, which is conducive to the development of degradable plastics. This study highlights the remarkable competence of nonconventional yeast for applications in biotechnology. |
| first_indexed | 2024-03-08T01:26:14Z |
| format | Article |
| id | doaj.art-d976b52b0fb045e39700e4d41f7419e8 |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-03-08T01:26:14Z |
| publishDate | 2024-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-d976b52b0fb045e39700e4d41f7419e82024-02-14T14:00:56ZengAmerican Society for MicrobiologymBio2150-75112024-02-0115210.1128/mbio.03196-23A robust yeast chassis: comprehensive characterization of a fast-growing Saccharomyces cerevisiaeYangdanyu Long0Xiao Han1Xuanlin Meng2Ping Xu3Fei Tao4State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, ChinaState Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, ChinaState Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, ChinaState Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, ChinaState Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, ChinaABSTRACTRobust chassis are critical to facilitate advances in synthetic biology. This study describes a comprehensive characterization of a new yeast isolate Saccharomyces cerevisiae XP that grows faster than commonly used research and industrial S. cerevisiae strains. The genomic, transcriptomic, and metabolomic analyses suggest that the fast growth rate is, in part, due to the efficient electron transport chain and key growth factor synthesis. A toolbox for genetic manipulation of the yeast was developed; we used it to construct l-lactic acid producers for high lactate production. The development of genetically malleable yeast strains that grow faster than currently used strains may significantly enhance the uses of S. cerevisiae in biotechnology.IMPORTANCEYeast is known as an outstanding starting strain for constructing microbial cell factories. However, its growth rate restricts its application. A yeast strain XP, which grows fast in high concentrations of sugar and acidic environments, is revealed to demonstrate the potential in industrial applications. A toolbox was also built for its genetic manipulation including gene insertion, deletion, and ploidy transformation. The knowledge of its metabolism, which could guide the designing of genetic experiments, was generated with multi-omics analyses. This novel strain along with its toolbox was then tested by constructing an l-lactic acid efficient producer, which is conducive to the development of degradable plastics. This study highlights the remarkable competence of nonconventional yeast for applications in biotechnology.https://journals.asm.org/doi/10.1128/mbio.03196-23Saccharomyces cerevisiaegrowth ratesynthetic biologychassisl-lactic acidrobustness |
| spellingShingle | Yangdanyu Long Xiao Han Xuanlin Meng Ping Xu Fei Tao A robust yeast chassis: comprehensive characterization of a fast-growing Saccharomyces cerevisiae mBio Saccharomyces cerevisiae growth rate synthetic biology chassis l-lactic acid robustness |
| title | A robust yeast chassis: comprehensive characterization of a fast-growing Saccharomyces cerevisiae |
| title_full | A robust yeast chassis: comprehensive characterization of a fast-growing Saccharomyces cerevisiae |
| title_fullStr | A robust yeast chassis: comprehensive characterization of a fast-growing Saccharomyces cerevisiae |
| title_full_unstemmed | A robust yeast chassis: comprehensive characterization of a fast-growing Saccharomyces cerevisiae |
| title_short | A robust yeast chassis: comprehensive characterization of a fast-growing Saccharomyces cerevisiae |
| title_sort | robust yeast chassis comprehensive characterization of a fast growing saccharomyces cerevisiae |
| topic | Saccharomyces cerevisiae growth rate synthetic biology chassis l-lactic acid robustness |
| url | https://journals.asm.org/doi/10.1128/mbio.03196-23 |
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