Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells
Eugenia uniflora Lam. is one of herbal products developed for anticancer. The aim of the present study was to identify the antiproliferative effect of ethanolic extract of Eugenia Uniflora Lam. leaves (EEU) on breast cancer cell line T47D. This Research was initiated by extracting the active content...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2012-06-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/58 |
| _version_ | 1818213317944016896 |
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| author | Nur Ismiyati Dyaningtyas Dewi Pamungkas Putri Siska Andrina Kusumastuti Rifki Febriansyah |
| author_facet | Nur Ismiyati Dyaningtyas Dewi Pamungkas Putri Siska Andrina Kusumastuti Rifki Febriansyah |
| author_sort | Nur Ismiyati |
| collection | DOAJ |
| description | Eugenia uniflora Lam. is one of herbal products developed for anticancer. The aim of the present study was to identify the antiproliferative effect of ethanolic extract of Eugenia Uniflora Lam. leaves (EEU) on breast cancer cell line T47D. This Research was initiated by extracting the active contents of Eugenia uniflora Lam. leaves by maceration with ethanol 96%. The extract was then analyzed by thin layer chromatography (TLC). Cytotoxic assay of EEU was carried out by using MTT assay. Apoptosis phenomenon was observed with double staining using acridine orange-ethidium bromide. EEU showed cytotoxic effect on T47D cells with IC50 value of 65 µg/ml. Moreover, EEU 50µg/ml and 100µg/ml induced apoptosis. TLC examination showed that EEU used in this study contain phenolic, flavonoid, and saponin compounds which were suggested to be responsible for antiproliferative effect. Further molecular mechanism underlying EEU antiproliferative effect needs to be done.
Keywords: Eugenia uniflora Lam., T47D cells, antiproliferative, apoptosis |
| first_indexed | 2024-12-12T06:02:23Z |
| format | Article |
| id | doaj.art-d9793f07d9424471b52824e556748496 |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-12-12T06:02:23Z |
| publishDate | 2012-06-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-d9793f07d9424471b52824e5567484962022-12-22T00:35:22ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892012-06-013237037510.14499/indonesianjcanchemoprev3iss2pp370-37559Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D CellsNur Ismiyati0Dyaningtyas Dewi Pamungkas Putri1Siska Andrina Kusumastuti2Rifki Febriansyah3Departement of Pharmacy, Politeknik Kesehatan Bhakti Setya IndonesiaCancer Chemoprevention Research Center Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaUniversitas Muhammadiyah YogyakartaEugenia uniflora Lam. is one of herbal products developed for anticancer. The aim of the present study was to identify the antiproliferative effect of ethanolic extract of Eugenia Uniflora Lam. leaves (EEU) on breast cancer cell line T47D. This Research was initiated by extracting the active contents of Eugenia uniflora Lam. leaves by maceration with ethanol 96%. The extract was then analyzed by thin layer chromatography (TLC). Cytotoxic assay of EEU was carried out by using MTT assay. Apoptosis phenomenon was observed with double staining using acridine orange-ethidium bromide. EEU showed cytotoxic effect on T47D cells with IC50 value of 65 µg/ml. Moreover, EEU 50µg/ml and 100µg/ml induced apoptosis. TLC examination showed that EEU used in this study contain phenolic, flavonoid, and saponin compounds which were suggested to be responsible for antiproliferative effect. Further molecular mechanism underlying EEU antiproliferative effect needs to be done. Keywords: Eugenia uniflora Lam., T47D cells, antiproliferative, apoptosishttps://ijcc.chemoprev.org/index.php/ijcc/article/view/58 |
| spellingShingle | Nur Ismiyati Dyaningtyas Dewi Pamungkas Putri Siska Andrina Kusumastuti Rifki Febriansyah Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells |
| title_full | Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells |
| title_fullStr | Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells |
| title_full_unstemmed | Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells |
| title_short | Antiproliferative Effect of Ethanolic Extract Eugenia uniflora Lam. Leaves on T47D Cells |
| title_sort | antiproliferative effect of ethanolic extract eugenia uniflora lam leaves on t47d cells |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/58 |
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