Elevated Sucrose-phosphate Synthase Activity in Source Leaves of Potato Plants Transrformed with the Maize SPS Gene
Sucrose-phosphate synthase (SPS) activity in source leaves of potato plants (Solanum tuberosum cv. May Queen) transformed with maize SPS gene under the control of a modified cauliflower mosaic virus (CaMV) 35S promoter was two to four times higher than the endogenous activity found in the control. A...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1999-01-01
|
| Series: | Plant Production Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1626/pps.2.92 |
| _version_ | 1818111091473907712 |
|---|---|
| author | Dennis J. Tobias Tatsuroh Hirose Ken Ishimaru Teruo Ishige Yasunobu Ohkawa Yuriko Kano-Murakami Makoto Matsuoka Ryu Ohsugi |
| author_facet | Dennis J. Tobias Tatsuroh Hirose Ken Ishimaru Teruo Ishige Yasunobu Ohkawa Yuriko Kano-Murakami Makoto Matsuoka Ryu Ohsugi |
| author_sort | Dennis J. Tobias |
| collection | DOAJ |
| description | Sucrose-phosphate synthase (SPS) activity in source leaves of potato plants (Solanum tuberosum cv. May Queen) transformed with maize SPS gene under the control of a modified cauliflower mosaic virus (CaMV) 35S promoter was two to four times higher than the endogenous activity found in the control. A negative correlation (r = 0.82) between SPS activity and the ratio of starch to sucrose was observed. There were no significant changes in starch synthesis enzyme (ADGPase and QE) activities in the leaves. Photosynthesis did not differ significantly between control and transgenic lines. Aboveground (leaves and stem) and belowground (tuber) dry matter in three transgenic lines harvested during tuber development increased up to 15% and 20%, respectively, indicating that increased capacity for sucrose synthesis in potato leaves may lead to favorable growth. Moreover, the activity of this enzyme in source leaves of two transgenic potatoes increased when reaction temperature was elevated from 15°C to 35°3, a response similar to that of SPS in maize. Since temperature is a limiting factor for growth of potato, the transgenic plants will be useful for future studies under higher temperature growth conditions. |
| first_indexed | 2024-12-11T02:57:32Z |
| format | Article |
| id | doaj.art-d97964756ab14020a063e513e5814fbd |
| institution | Directory Open Access Journal |
| issn | 1343-943X 1349-1008 |
| language | English |
| last_indexed | 2024-12-11T02:57:32Z |
| publishDate | 1999-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Production Science |
| spelling | doaj.art-d97964756ab14020a063e513e5814fbd2022-12-22T01:23:07ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10081999-01-0122929910.1626/pps.2.9211644356Elevated Sucrose-phosphate Synthase Activity in Source Leaves of Potato Plants Transrformed with the Maize SPS GeneDennis J. Tobias0Tatsuroh Hirose1Ken Ishimaru2Teruo Ishige3Yasunobu Ohkawa4Yuriko Kano-Murakami5Makoto Matsuoka6Ryu Ohsugi7National Institute of Agrobiological Resources (JVIAR)Hokuriku National Agricultural Experiment StationNational Institute of Agrobiological Resources (JVIAR)Japan International Research Center for Agricultural Sciences (JIRCAS)National Institute of Agrobiological Resources (JVIAR)National Institute of Fruit Tree ScienceNagoya UniversityNational Institute of Agrobiological Resources (JVIAR)Sucrose-phosphate synthase (SPS) activity in source leaves of potato plants (Solanum tuberosum cv. May Queen) transformed with maize SPS gene under the control of a modified cauliflower mosaic virus (CaMV) 35S promoter was two to four times higher than the endogenous activity found in the control. A negative correlation (r = 0.82) between SPS activity and the ratio of starch to sucrose was observed. There were no significant changes in starch synthesis enzyme (ADGPase and QE) activities in the leaves. Photosynthesis did not differ significantly between control and transgenic lines. Aboveground (leaves and stem) and belowground (tuber) dry matter in three transgenic lines harvested during tuber development increased up to 15% and 20%, respectively, indicating that increased capacity for sucrose synthesis in potato leaves may lead to favorable growth. Moreover, the activity of this enzyme in source leaves of two transgenic potatoes increased when reaction temperature was elevated from 15°C to 35°3, a response similar to that of SPS in maize. Since temperature is a limiting factor for growth of potato, the transgenic plants will be useful for future studies under higher temperature growth conditions.http://dx.doi.org/10.1626/pps.2.92GrowthSolanum tuberosum (cv. May Queen)Source leafSucrose and starch partitioningSucrose phosphate synthase |
| spellingShingle | Dennis J. Tobias Tatsuroh Hirose Ken Ishimaru Teruo Ishige Yasunobu Ohkawa Yuriko Kano-Murakami Makoto Matsuoka Ryu Ohsugi Elevated Sucrose-phosphate Synthase Activity in Source Leaves of Potato Plants Transrformed with the Maize SPS Gene Plant Production Science Growth Solanum tuberosum (cv. May Queen) Source leaf Sucrose and starch partitioning Sucrose phosphate synthase |
| title | Elevated Sucrose-phosphate Synthase Activity in Source Leaves of Potato Plants Transrformed with the Maize SPS Gene |
| title_full | Elevated Sucrose-phosphate Synthase Activity in Source Leaves of Potato Plants Transrformed with the Maize SPS Gene |
| title_fullStr | Elevated Sucrose-phosphate Synthase Activity in Source Leaves of Potato Plants Transrformed with the Maize SPS Gene |
| title_full_unstemmed | Elevated Sucrose-phosphate Synthase Activity in Source Leaves of Potato Plants Transrformed with the Maize SPS Gene |
| title_short | Elevated Sucrose-phosphate Synthase Activity in Source Leaves of Potato Plants Transrformed with the Maize SPS Gene |
| title_sort | elevated sucrose phosphate synthase activity in source leaves of potato plants transrformed with the maize sps gene |
| topic | Growth Solanum tuberosum (cv. May Queen) Source leaf Sucrose and starch partitioning Sucrose phosphate synthase |
| url | http://dx.doi.org/10.1626/pps.2.92 |
| work_keys_str_mv | AT dennisjtobias elevatedsucrosephosphatesynthaseactivityinsourceleavesofpotatoplantstransrformedwiththemaizespsgene AT tatsurohhirose elevatedsucrosephosphatesynthaseactivityinsourceleavesofpotatoplantstransrformedwiththemaizespsgene AT kenishimaru elevatedsucrosephosphatesynthaseactivityinsourceleavesofpotatoplantstransrformedwiththemaizespsgene AT teruoishige elevatedsucrosephosphatesynthaseactivityinsourceleavesofpotatoplantstransrformedwiththemaizespsgene AT yasunobuohkawa elevatedsucrosephosphatesynthaseactivityinsourceleavesofpotatoplantstransrformedwiththemaizespsgene AT yurikokanomurakami elevatedsucrosephosphatesynthaseactivityinsourceleavesofpotatoplantstransrformedwiththemaizespsgene AT makotomatsuoka elevatedsucrosephosphatesynthaseactivityinsourceleavesofpotatoplantstransrformedwiththemaizespsgene AT ryuohsugi elevatedsucrosephosphatesynthaseactivityinsourceleavesofpotatoplantstransrformedwiththemaizespsgene |