Synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challenge
Abstract Objective Our study aims to synthesize, characterize, and determine the effects of a ChNPs suspension on human enamel after cariogenic challenge via pH-cycling. Methodology ChNPs were synthesized by ion gelation and characterized by Transmission Electron Microscopy (TEM) and Dynamic Lig...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2021-10-01
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572021000100452&tlng=en |
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| author | Taís Chaves MAGALHÃES Natália Moreira TEIXEIRA Renata Sobreira FRANÇA Ângelo Márcio Leite DENADAI Rogério Lacerda dos SANTOS Hugo Lemes CARLO Eliseu Aldrighi MUNCHOW Fabíola Galbiatti de CARVALHO |
| author_facet | Taís Chaves MAGALHÃES Natália Moreira TEIXEIRA Renata Sobreira FRANÇA Ângelo Márcio Leite DENADAI Rogério Lacerda dos SANTOS Hugo Lemes CARLO Eliseu Aldrighi MUNCHOW Fabíola Galbiatti de CARVALHO |
| author_sort | Taís Chaves MAGALHÃES |
| collection | DOAJ |
| description | Abstract Objective Our study aims to synthesize, characterize, and determine the effects of a ChNPs suspension on human enamel after cariogenic challenge via pH-cycling. Methodology ChNPs were synthesized by ion gelation and characterized by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering. Forty enamel blocks were divided into four groups (n=10/group): (i) ChNPs suspension; (ii) chitosan solution; (iii) 0.05% sodium fluoride (NaF) solution; and (iv) distilled water. Specimens were exposed to cariogenic challenge by cycling in demineralization solution (3 h) and then remineralized (21h) for 7 days. Before each demineralization cycle, the corresponding solutions were passively applied for 90 s. After 7 days, specimens were examined for surface roughness (Ra) and Knoop hardness (KHN) before and after the cariogenic challenge; % KHN change (variation between initial and final hardness), and surface topography by an optical profilometer. The data were analyzed by repeated-measures ANOVA, One-way ANOVA, and Tukey tests (α=0.05). Results TEM images showed small spherical particles with diameter and zeta potential values of 79.3 nm and +47.9 mV, respectively. After the challenge, all groups showed an increase in Ra and a decrease in KHN values. Optical profilometry indicated that ChNPs- and NaF-treated specimens showed uneven roughness interspersed with smooth areas and the lowest %KHN values. Conclusion The ChNPs suspension was successfully synthesized and minimized human enamel demineralization after a cariogenic challenge, showing an interesting potential for use as an oral formulation for caries prevention. |
| first_indexed | 2024-12-20T10:34:41Z |
| format | Article |
| id | doaj.art-d97fb409b2bd4cbaac6ff0923ae16c68 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-12-20T10:34:41Z |
| publishDate | 2021-10-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-d97fb409b2bd4cbaac6ff0923ae16c682022-12-21T19:43:38ZengUniversity of São PauloJournal of Applied Oral Science1678-77652021-10-012910.1590/1678-7757-2021-0120Synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challengeTaís Chaves MAGALHÃEShttps://orcid.org/0000-0003-1920-1745Natália Moreira TEIXEIRARenata Sobreira FRANÇAÂngelo Márcio Leite DENADAIRogério Lacerda dos SANTOShttps://orcid.org/0000-0002-6213-9206Hugo Lemes CARLOEliseu Aldrighi MUNCHOWFabíola Galbiatti de CARVALHOhttps://orcid.org/0000-0003-2510-1329Abstract Objective Our study aims to synthesize, characterize, and determine the effects of a ChNPs suspension on human enamel after cariogenic challenge via pH-cycling. Methodology ChNPs were synthesized by ion gelation and characterized by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering. Forty enamel blocks were divided into four groups (n=10/group): (i) ChNPs suspension; (ii) chitosan solution; (iii) 0.05% sodium fluoride (NaF) solution; and (iv) distilled water. Specimens were exposed to cariogenic challenge by cycling in demineralization solution (3 h) and then remineralized (21h) for 7 days. Before each demineralization cycle, the corresponding solutions were passively applied for 90 s. After 7 days, specimens were examined for surface roughness (Ra) and Knoop hardness (KHN) before and after the cariogenic challenge; % KHN change (variation between initial and final hardness), and surface topography by an optical profilometer. The data were analyzed by repeated-measures ANOVA, One-way ANOVA, and Tukey tests (α=0.05). Results TEM images showed small spherical particles with diameter and zeta potential values of 79.3 nm and +47.9 mV, respectively. After the challenge, all groups showed an increase in Ra and a decrease in KHN values. Optical profilometry indicated that ChNPs- and NaF-treated specimens showed uneven roughness interspersed with smooth areas and the lowest %KHN values. Conclusion The ChNPs suspension was successfully synthesized and minimized human enamel demineralization after a cariogenic challenge, showing an interesting potential for use as an oral formulation for caries prevention.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572021000100452&tlng=enChitosanNanoparticlesDental cariesDental enamelHardness |
| spellingShingle | Taís Chaves MAGALHÃES Natália Moreira TEIXEIRA Renata Sobreira FRANÇA Ângelo Márcio Leite DENADAI Rogério Lacerda dos SANTOS Hugo Lemes CARLO Eliseu Aldrighi MUNCHOW Fabíola Galbiatti de CARVALHO Synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challenge Journal of Applied Oral Science Chitosan Nanoparticles Dental caries Dental enamel Hardness |
| title | Synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challenge |
| title_full | Synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challenge |
| title_fullStr | Synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challenge |
| title_full_unstemmed | Synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challenge |
| title_short | Synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challenge |
| title_sort | synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challenge |
| topic | Chitosan Nanoparticles Dental caries Dental enamel Hardness |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572021000100452&tlng=en |
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