Draft Genome Sequence of a New Fusarium Isolate Belonging to Fusarium tricinctum Species Complex Collected From Hazelnut in Central Italy
In summer 2019, during a survey on the health status of a hazelnut orchard located in the Tuscia area (the province of Viterbo, Latium, Italy), nuts showing symptoms, such as brown-grayish spots at the bottom of the nuts progressing upward to the apex, and necrotic patches on the bracts and, sometim...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.788584/full |
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| author | Silvia Turco Alessandro Grottoli Mounira Inas Drais Carlo De Spirito Luigi Faino Massimo Reverberi Valerio Cristofori Angelo Mazzaglia |
| author_facet | Silvia Turco Alessandro Grottoli Mounira Inas Drais Carlo De Spirito Luigi Faino Massimo Reverberi Valerio Cristofori Angelo Mazzaglia |
| author_sort | Silvia Turco |
| collection | DOAJ |
| description | In summer 2019, during a survey on the health status of a hazelnut orchard located in the Tuscia area (the province of Viterbo, Latium, Italy), nuts showing symptoms, such as brown-grayish spots at the bottom of the nuts progressing upward to the apex, and necrotic patches on the bracts and, sometimes, on the petioles, were found and collected for further studies. This syndrome is associated with the nut gray necrosis (NGN), whose main causal agent is Fusarium lateritium. Aiming to increase knowledge about this fungal pathogen, the whole-genome sequencing of a strain isolated from symptomatic hazelnut was performed using long Nanopore reads technology in combination with the higher precision of the Illumina reads, generating a high-quality genome assembly. The following phylogenetic and comparative genomics analysis suggested that this isolate is caused by the F. tricinctum species complex rather than F. lateritium one, as initially hypothesized. Thus, this study demonstrates that different Fusarium species can infect Corylus avellana producing the same symptomatology. In addition, it sheds light onto the genetic features of the pathogen in subject, clarifying facets about its biology, epidemiology, infection mechanisms, and host spectrum, with the future objective to develop specific and efficient control strategies. |
| first_indexed | 2024-12-13T21:18:12Z |
| format | Article |
| id | doaj.art-d9814beeeb8e421b959724d174be41d2 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T21:18:12Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d9814beeeb8e421b959724d174be41d22022-12-21T23:31:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-12-011210.3389/fpls.2021.788584788584Draft Genome Sequence of a New Fusarium Isolate Belonging to Fusarium tricinctum Species Complex Collected From Hazelnut in Central ItalySilvia Turco0Alessandro Grottoli1Mounira Inas Drais2Carlo De Spirito3Luigi Faino4Massimo Reverberi5Valerio Cristofori6Angelo Mazzaglia7Dipartimento di Scienze Agrarie e Forestali, Università degli Studi della Tuscia, Viterbo, ItalyConsiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria, Centro di Ricerca Difesa e Certificazione (CREA-DC), Rome, ItalyDipartimento di Scienze Agrarie e Forestali, Università degli Studi della Tuscia, Viterbo, ItalyDipartimento di Scienze Agrarie e Forestali, Università degli Studi della Tuscia, Viterbo, ItalyDipartimento di Biologia Ambientale, Sapienza Università di Roma, Rome, ItalyDipartimento di Biologia Ambientale, Sapienza Università di Roma, Rome, ItalyDipartimento di Scienze Agrarie e Forestali, Università degli Studi della Tuscia, Viterbo, ItalyDipartimento di Scienze Agrarie e Forestali, Università degli Studi della Tuscia, Viterbo, ItalyIn summer 2019, during a survey on the health status of a hazelnut orchard located in the Tuscia area (the province of Viterbo, Latium, Italy), nuts showing symptoms, such as brown-grayish spots at the bottom of the nuts progressing upward to the apex, and necrotic patches on the bracts and, sometimes, on the petioles, were found and collected for further studies. This syndrome is associated with the nut gray necrosis (NGN), whose main causal agent is Fusarium lateritium. Aiming to increase knowledge about this fungal pathogen, the whole-genome sequencing of a strain isolated from symptomatic hazelnut was performed using long Nanopore reads technology in combination with the higher precision of the Illumina reads, generating a high-quality genome assembly. The following phylogenetic and comparative genomics analysis suggested that this isolate is caused by the F. tricinctum species complex rather than F. lateritium one, as initially hypothesized. Thus, this study demonstrates that different Fusarium species can infect Corylus avellana producing the same symptomatology. In addition, it sheds light onto the genetic features of the pathogen in subject, clarifying facets about its biology, epidemiology, infection mechanisms, and host spectrum, with the future objective to develop specific and efficient control strategies.https://www.frontiersin.org/articles/10.3389/fpls.2021.788584/fullnut gray necrosis (NGN)Fusarium tricinctum species complexhazelnut (Corylus avellana L.)genomicshybrid assembly |
| spellingShingle | Silvia Turco Alessandro Grottoli Mounira Inas Drais Carlo De Spirito Luigi Faino Massimo Reverberi Valerio Cristofori Angelo Mazzaglia Draft Genome Sequence of a New Fusarium Isolate Belonging to Fusarium tricinctum Species Complex Collected From Hazelnut in Central Italy Frontiers in Plant Science nut gray necrosis (NGN) Fusarium tricinctum species complex hazelnut (Corylus avellana L.) genomics hybrid assembly |
| title | Draft Genome Sequence of a New Fusarium Isolate Belonging to Fusarium tricinctum Species Complex Collected From Hazelnut in Central Italy |
| title_full | Draft Genome Sequence of a New Fusarium Isolate Belonging to Fusarium tricinctum Species Complex Collected From Hazelnut in Central Italy |
| title_fullStr | Draft Genome Sequence of a New Fusarium Isolate Belonging to Fusarium tricinctum Species Complex Collected From Hazelnut in Central Italy |
| title_full_unstemmed | Draft Genome Sequence of a New Fusarium Isolate Belonging to Fusarium tricinctum Species Complex Collected From Hazelnut in Central Italy |
| title_short | Draft Genome Sequence of a New Fusarium Isolate Belonging to Fusarium tricinctum Species Complex Collected From Hazelnut in Central Italy |
| title_sort | draft genome sequence of a new fusarium isolate belonging to fusarium tricinctum species complex collected from hazelnut in central italy |
| topic | nut gray necrosis (NGN) Fusarium tricinctum species complex hazelnut (Corylus avellana L.) genomics hybrid assembly |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.788584/full |
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