| Summary: | Cryptochrome (CRY) is a kind of flavin-binding protein that can sense blue light and near-ultraviolet light, and participates in the light response of organisms and the regulation of the circadian clock. The complete open reading frame (ORF) of <i>CiPlant-CRY1</i> (GenBank ID OM389130.1), encoding one kind of CRY, was cloned from the Antarctic ice alga <i>Chlamydomonas</i> sp. ICE-L. The quantitative real-time PCR study showed that the expression level of the <i>CiPlant-CRY1</i> gene was the highest at 5 °C and salinity of 32‰. <i>CiPlant-CRY1</i> was positively regulated by blue or yellow light, suggesting that it is involved in the establishment of photomorphology. The <i>CiPlant-CRY1</i> gene can respond to polar day and polar night, indicating its expression is regulated by circadian rhythm. The expression level of <i>CiPlant-CRY1</i> was most affected by UVB irradiation, which may be related to the adaptation of ice algae to a strong ultraviolet radiation environment. Moreover, the recombinant protein of <i>CiPlant-CRY1</i> was expressed by prokaryotic expression. This study may be important for exploring the light-induced rhythm regulation of Antarctic ice algae in the polar marine environment.
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