Genome-wide identification and expression profiling of the ABI5 gene family in foxtail millet (Setaria italica)
Abstract Background ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that crucially influences plant growth, development, and stress response. However, there is minimal research on the ABI5 family in foxtail millet. Results In this study, 16 ABI5 genes were identified in foxta...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-04865-4 |
| _version_ | 1797275312889790464 |
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| author | Yinyuan Wen Zeya Zhao Liuna Cheng Shixue Zhou Mengyao An Juan Zhao Shuqi Dong Xiangyang Yuan Meiqiang Yin |
| author_facet | Yinyuan Wen Zeya Zhao Liuna Cheng Shixue Zhou Mengyao An Juan Zhao Shuqi Dong Xiangyang Yuan Meiqiang Yin |
| author_sort | Yinyuan Wen |
| collection | DOAJ |
| description | Abstract Background ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that crucially influences plant growth, development, and stress response. However, there is minimal research on the ABI5 family in foxtail millet. Results In this study, 16 ABI5 genes were identified in foxtail millet, and their sequence composition, gene structures, cis-acting elements, chromosome positions, and gene replication events were analyzed. To more thoroughly evaluate the developmental mechanisms of the SiABI5 family during evolution, we selected three dicotyledons (S. lycopersicum, A. thaliana, F. tataricum) and three (Z. mays, O. sativa, S. bicolor) specific representative monocotyledons associated with foxtail millet for comparative homology mapping. The results showed that foxtail millet ABI5 genes had the best homology with maize. A promoter sequence analysis showed that the SiABI5s contain numerous cis-acting elements related to hormone and stress responses, indicating that the regulation of SiABI5 expression was complex. The expression responses of 16 genes in different tissues, seed germination, and ear development were analyzed. A total of six representative genes were targeted from five subfamilies to characterize their gene expression responses to four different abiotic stresses. Overexpression of SiABI5.12 confers tolerance to osmotic stress in transgenic Arabidopsis thaliana, which demonstrated the function of SiABI5 responded to abiotic stress. Conclusions In summary, our research results comprehensively characterized the SiABI5 family and can provide a valuable reference for demonstrating the role of SiABI5s in regulating abiotic stress responses in foxtail millet. |
| first_indexed | 2024-03-07T15:11:30Z |
| format | Article |
| id | doaj.art-d9835cdcffd349b494f74dffe3f34b40 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-07T15:11:30Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-d9835cdcffd349b494f74dffe3f34b402024-03-05T18:38:27ZengBMCBMC Plant Biology1471-22292024-03-0124111710.1186/s12870-024-04865-4Genome-wide identification and expression profiling of the ABI5 gene family in foxtail millet (Setaria italica)Yinyuan Wen0Zeya Zhao1Liuna Cheng2Shixue Zhou3Mengyao An4Juan Zhao5Shuqi Dong6Xiangyang Yuan7Meiqiang Yin8College of Agronomy, Shanxi Agricultural UniversityCollege of Agronomy, Shanxi Agricultural UniversityCollege of Agronomy, Shanxi Agricultural UniversityCollege of Agronomy, Shanxi Agricultural UniversityCollege of Agronomy, Shanxi Agricultural UniversityCollege of Agronomy, Shanxi Agricultural UniversityCollege of Agronomy, Shanxi Agricultural UniversityCollege of Agronomy, Shanxi Agricultural UniversityCollege of Agronomy, Shanxi Agricultural UniversityAbstract Background ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that crucially influences plant growth, development, and stress response. However, there is minimal research on the ABI5 family in foxtail millet. Results In this study, 16 ABI5 genes were identified in foxtail millet, and their sequence composition, gene structures, cis-acting elements, chromosome positions, and gene replication events were analyzed. To more thoroughly evaluate the developmental mechanisms of the SiABI5 family during evolution, we selected three dicotyledons (S. lycopersicum, A. thaliana, F. tataricum) and three (Z. mays, O. sativa, S. bicolor) specific representative monocotyledons associated with foxtail millet for comparative homology mapping. The results showed that foxtail millet ABI5 genes had the best homology with maize. A promoter sequence analysis showed that the SiABI5s contain numerous cis-acting elements related to hormone and stress responses, indicating that the regulation of SiABI5 expression was complex. The expression responses of 16 genes in different tissues, seed germination, and ear development were analyzed. A total of six representative genes were targeted from five subfamilies to characterize their gene expression responses to four different abiotic stresses. Overexpression of SiABI5.12 confers tolerance to osmotic stress in transgenic Arabidopsis thaliana, which demonstrated the function of SiABI5 responded to abiotic stress. Conclusions In summary, our research results comprehensively characterized the SiABI5 family and can provide a valuable reference for demonstrating the role of SiABI5s in regulating abiotic stress responses in foxtail millet.https://doi.org/10.1186/s12870-024-04865-4Foxtail milletSiABI5Phylogenetic analysisExpression analysisAbiotic stress |
| spellingShingle | Yinyuan Wen Zeya Zhao Liuna Cheng Shixue Zhou Mengyao An Juan Zhao Shuqi Dong Xiangyang Yuan Meiqiang Yin Genome-wide identification and expression profiling of the ABI5 gene family in foxtail millet (Setaria italica) BMC Plant Biology Foxtail millet SiABI5 Phylogenetic analysis Expression analysis Abiotic stress |
| title | Genome-wide identification and expression profiling of the ABI5 gene family in foxtail millet (Setaria italica) |
| title_full | Genome-wide identification and expression profiling of the ABI5 gene family in foxtail millet (Setaria italica) |
| title_fullStr | Genome-wide identification and expression profiling of the ABI5 gene family in foxtail millet (Setaria italica) |
| title_full_unstemmed | Genome-wide identification and expression profiling of the ABI5 gene family in foxtail millet (Setaria italica) |
| title_short | Genome-wide identification and expression profiling of the ABI5 gene family in foxtail millet (Setaria italica) |
| title_sort | genome wide identification and expression profiling of the abi5 gene family in foxtail millet setaria italica |
| topic | Foxtail millet SiABI5 Phylogenetic analysis Expression analysis Abiotic stress |
| url | https://doi.org/10.1186/s12870-024-04865-4 |
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