Anti-Atopic Dermatitis Activity of <i>Cornus walteri</i> and Identification of the Bioactive Compounds
Atopic dermatitis (AD) is a chronic inflammatory disease characterized by hyperactivated immune reactions in the skin. AD is a prevalent health concern in developing countries, with a particularly high incidence among children. Despite ongoing research on AD, prevention and treatment strategies for...
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MDPI AG
2022-09-01
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| author | Bum Soo Lee Ye-Ryeong Cho Minju Jeong Yoon Seo Jang Jin-Chul Kim Sanguine Byun Ki Hyun Kim |
| author_facet | Bum Soo Lee Ye-Ryeong Cho Minju Jeong Yoon Seo Jang Jin-Chul Kim Sanguine Byun Ki Hyun Kim |
| author_sort | Bum Soo Lee |
| collection | DOAJ |
| description | Atopic dermatitis (AD) is a chronic inflammatory disease characterized by hyperactivated immune reactions in the skin. AD is a prevalent health concern in developing countries, with a particularly high incidence among children. Despite ongoing research on AD, prevention and treatment strategies for patients remain inadequate. In this study, the inhibitory effects of <i>Cornus walteri</i> on AD were investigated. <i>C. walteri</i> (Cornaceae), commonly known as “Walter’s dogwood,” is a deciduous shrub used as a traditional medicine to treat dermatologic inflammation caused by lacquer poisoning. However, the role of <i>C. walteri</i> in AD remains largely unknown. To evaluate its anti-AD potential, we investigated the anti-inflammatory activity of the MeOH extract of <i>C. walteri</i> stems (CWS) using the HaCaT human keratinocyte cell line. CWS reduced the secretion of AD-related chemokines, eotaxin-3/CCL26 and thymus and activation-regulated chemokine (TARC/CCL17). In addition, CWS also inhibited the mRNA expression of macrophage-derived chemokine (MDC/CCL22) and upregulated filaggrin, which plays an essential role in skin barrier functions. To identify the bioactive constituents of CWS, phytochemical investigation of CWS led to the isolation of potential bioactive constituents (<b>1</b>–<b>6</b>), including four triterpenoids, one steroid and one diterpene analog, the structures of which were identified as lupeol (<b>1</b>), betulinic acid (<b>2</b>), 5<i>α</i>-stigmast-3,6-dione (<b>3</b>), 3-<i>O</i>-acetylbetulin (<b>4</b>), betulinic acid methyl ester (<b>5</b>) and norphytan (<b>6</b>) through nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography (LC)–mass spectrometry (MS) analysis. The isolated compounds (<b>1</b>–<b>6</b>) were evaluated for their inhibitory activities against eotaxin-3 expression. Compounds <b>1</b>, <b>2</b> and <b>3</b> significantly reduced the levels of eotaxin-3. These findings provided experimental evidence that CWS, particularly active compounds <b>1</b>, <b>2</b> and <b>3</b>, could be further utilized as potential therapeutic agents to treat AD. |
| first_indexed | 2024-03-10T03:00:13Z |
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| spelling | doaj.art-d98735bc60f149e7868939ee2474564d2023-11-23T12:48:05ZengMDPI AGApplied Sciences2076-34172022-09-011217885710.3390/app12178857Anti-Atopic Dermatitis Activity of <i>Cornus walteri</i> and Identification of the Bioactive CompoundsBum Soo Lee0Ye-Ryeong Cho1Minju Jeong2Yoon Seo Jang3Jin-Chul Kim4Sanguine Byun5Ki Hyun Kim6School of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Biotechnology, Yonsei University, Seoul 03722, KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaKIST Gangneung Institute of Natural Products, Natural Product Informatics Research Center, Gangneung 25451, KoreaDepartment of Biotechnology, Yonsei University, Seoul 03722, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaAtopic dermatitis (AD) is a chronic inflammatory disease characterized by hyperactivated immune reactions in the skin. AD is a prevalent health concern in developing countries, with a particularly high incidence among children. Despite ongoing research on AD, prevention and treatment strategies for patients remain inadequate. In this study, the inhibitory effects of <i>Cornus walteri</i> on AD were investigated. <i>C. walteri</i> (Cornaceae), commonly known as “Walter’s dogwood,” is a deciduous shrub used as a traditional medicine to treat dermatologic inflammation caused by lacquer poisoning. However, the role of <i>C. walteri</i> in AD remains largely unknown. To evaluate its anti-AD potential, we investigated the anti-inflammatory activity of the MeOH extract of <i>C. walteri</i> stems (CWS) using the HaCaT human keratinocyte cell line. CWS reduced the secretion of AD-related chemokines, eotaxin-3/CCL26 and thymus and activation-regulated chemokine (TARC/CCL17). In addition, CWS also inhibited the mRNA expression of macrophage-derived chemokine (MDC/CCL22) and upregulated filaggrin, which plays an essential role in skin barrier functions. To identify the bioactive constituents of CWS, phytochemical investigation of CWS led to the isolation of potential bioactive constituents (<b>1</b>–<b>6</b>), including four triterpenoids, one steroid and one diterpene analog, the structures of which were identified as lupeol (<b>1</b>), betulinic acid (<b>2</b>), 5<i>α</i>-stigmast-3,6-dione (<b>3</b>), 3-<i>O</i>-acetylbetulin (<b>4</b>), betulinic acid methyl ester (<b>5</b>) and norphytan (<b>6</b>) through nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography (LC)–mass spectrometry (MS) analysis. The isolated compounds (<b>1</b>–<b>6</b>) were evaluated for their inhibitory activities against eotaxin-3 expression. Compounds <b>1</b>, <b>2</b> and <b>3</b> significantly reduced the levels of eotaxin-3. These findings provided experimental evidence that CWS, particularly active compounds <b>1</b>, <b>2</b> and <b>3</b>, could be further utilized as potential therapeutic agents to treat AD.https://www.mdpi.com/2076-3417/12/17/8857Walter’s dogwoodatopic dermatitiskeratinocyteschemokinefilaggrin |
| spellingShingle | Bum Soo Lee Ye-Ryeong Cho Minju Jeong Yoon Seo Jang Jin-Chul Kim Sanguine Byun Ki Hyun Kim Anti-Atopic Dermatitis Activity of <i>Cornus walteri</i> and Identification of the Bioactive Compounds Applied Sciences Walter’s dogwood atopic dermatitis keratinocytes chemokine filaggrin |
| title | Anti-Atopic Dermatitis Activity of <i>Cornus walteri</i> and Identification of the Bioactive Compounds |
| title_full | Anti-Atopic Dermatitis Activity of <i>Cornus walteri</i> and Identification of the Bioactive Compounds |
| title_fullStr | Anti-Atopic Dermatitis Activity of <i>Cornus walteri</i> and Identification of the Bioactive Compounds |
| title_full_unstemmed | Anti-Atopic Dermatitis Activity of <i>Cornus walteri</i> and Identification of the Bioactive Compounds |
| title_short | Anti-Atopic Dermatitis Activity of <i>Cornus walteri</i> and Identification of the Bioactive Compounds |
| title_sort | anti atopic dermatitis activity of i cornus walteri i and identification of the bioactive compounds |
| topic | Walter’s dogwood atopic dermatitis keratinocytes chemokine filaggrin |
| url | https://www.mdpi.com/2076-3417/12/17/8857 |
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