Mining of Minor Disease Resistance Genes in <i>V. vinifera</i> Grapes Based on Transcriptome
Intraspecific recurrent selection in <i>V. vinifera</i> is an effective method for grape breeding with high quality and disease resistance. The core theory of this method is the substitution accumulation of multi-genes with low disease resistance. The discovery of multi-genes for disease...
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/20/15311 |
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| author | Junli Liu Liang Wang Shan Jiang Zhilei Wang Hua Li Hua Wang |
| author_facet | Junli Liu Liang Wang Shan Jiang Zhilei Wang Hua Li Hua Wang |
| author_sort | Junli Liu |
| collection | DOAJ |
| description | Intraspecific recurrent selection in <i>V. vinifera</i> is an effective method for grape breeding with high quality and disease resistance. The core theory of this method is the substitution accumulation of multi-genes with low disease resistance. The discovery of multi-genes for disease resistance in <i>V. vinifera</i> may provide a molecular basis for breeding for disease resistance in <i>V. vinifera.</i> In this study, resistance to downy mildew was identified, and genetic analysis was carried out in the intraspecific crossing population of <i>V. vinifera</i> (<i>Ecolly</i> × <i>Dunkelfelder</i>) to screen immune, highly resistant and disease-resistant plant samples; transcriptome sequencing and differential expression analysis were performed using high-throughput sequencing. The results showed that there were 546 differential genes (194 up-regulated and 352 down-regulated) in the immune group compared to the highly resistant group, and 199 differential genes (50 up-regulated and 149 down-regulated) in the highly resistant group compared to the resistant group, there were 103 differential genes (54 up-regulated and 49 down-regulated) in the immune group compared to the resistant group. KEGG analysis of differentially expressed genes in the immune versus high-resistance group. The pathway is mainly concentrated in phenylpropanoid biosynthesis, starch and sucrose metabolism, MAPK signaling pathway–plant, carotenoid biosyn-thesis and isoquinoline alkaloid biosynthesis. The differential gene functions of immune and resistant, high-resistant and resistant combinations were mainly enriched in plant–pathogen interaction pathway. Through the analysis of disease resistance-related genes in each pathway, the potential minor resistance genes in <i>V. vinifera</i> were mined, and the accumulation of minor resistance genes was analyzed from the molecular level. |
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| language | English |
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| publishDate | 2023-10-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d9882cde074b4a09af164afbe0140d772023-11-19T16:44:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201531110.3390/ijms242015311Mining of Minor Disease Resistance Genes in <i>V. vinifera</i> Grapes Based on TranscriptomeJunli Liu0Liang Wang1Shan Jiang2Zhilei Wang3Hua Li4Hua Wang5College of Enology, Northwest A&F University, Xianyang 712100, ChinaCollege of Enology, Northwest A&F University, Xianyang 712100, ChinaCollege of Enology, Northwest A&F University, Xianyang 712100, ChinaCollege of Enology, Northwest A&F University, Xianyang 712100, ChinaCollege of Enology, Northwest A&F University, Xianyang 712100, ChinaCollege of Enology, Northwest A&F University, Xianyang 712100, ChinaIntraspecific recurrent selection in <i>V. vinifera</i> is an effective method for grape breeding with high quality and disease resistance. The core theory of this method is the substitution accumulation of multi-genes with low disease resistance. The discovery of multi-genes for disease resistance in <i>V. vinifera</i> may provide a molecular basis for breeding for disease resistance in <i>V. vinifera.</i> In this study, resistance to downy mildew was identified, and genetic analysis was carried out in the intraspecific crossing population of <i>V. vinifera</i> (<i>Ecolly</i> × <i>Dunkelfelder</i>) to screen immune, highly resistant and disease-resistant plant samples; transcriptome sequencing and differential expression analysis were performed using high-throughput sequencing. The results showed that there were 546 differential genes (194 up-regulated and 352 down-regulated) in the immune group compared to the highly resistant group, and 199 differential genes (50 up-regulated and 149 down-regulated) in the highly resistant group compared to the resistant group, there were 103 differential genes (54 up-regulated and 49 down-regulated) in the immune group compared to the resistant group. KEGG analysis of differentially expressed genes in the immune versus high-resistance group. The pathway is mainly concentrated in phenylpropanoid biosynthesis, starch and sucrose metabolism, MAPK signaling pathway–plant, carotenoid biosyn-thesis and isoquinoline alkaloid biosynthesis. The differential gene functions of immune and resistant, high-resistant and resistant combinations were mainly enriched in plant–pathogen interaction pathway. Through the analysis of disease resistance-related genes in each pathway, the potential minor resistance genes in <i>V. vinifera</i> were mined, and the accumulation of minor resistance genes was analyzed from the molecular level.https://www.mdpi.com/1422-0067/24/20/15311<i>V. vinifera</i>disease resistancemicroactive polygenestranscriptome |
| spellingShingle | Junli Liu Liang Wang Shan Jiang Zhilei Wang Hua Li Hua Wang Mining of Minor Disease Resistance Genes in <i>V. vinifera</i> Grapes Based on Transcriptome International Journal of Molecular Sciences <i>V. vinifera</i> disease resistance microactive polygenes transcriptome |
| title | Mining of Minor Disease Resistance Genes in <i>V. vinifera</i> Grapes Based on Transcriptome |
| title_full | Mining of Minor Disease Resistance Genes in <i>V. vinifera</i> Grapes Based on Transcriptome |
| title_fullStr | Mining of Minor Disease Resistance Genes in <i>V. vinifera</i> Grapes Based on Transcriptome |
| title_full_unstemmed | Mining of Minor Disease Resistance Genes in <i>V. vinifera</i> Grapes Based on Transcriptome |
| title_short | Mining of Minor Disease Resistance Genes in <i>V. vinifera</i> Grapes Based on Transcriptome |
| title_sort | mining of minor disease resistance genes in i v vinifera i grapes based on transcriptome |
| topic | <i>V. vinifera</i> disease resistance microactive polygenes transcriptome |
| url | https://www.mdpi.com/1422-0067/24/20/15311 |
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