Suppressive effect of azithromycin on <it>Plasmodium berghei </it>mosquito stage development and apicoplast replication
<p>Abstract</p> <p>Background</p> <p>Azithromycin (AZM) is a macrolide antibiotic that displays an excellent safety profile even in children and pregnant women and has been shown to have anti-malarial activity against blood stage <it>Plasmodium falciparum</it&g...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-03-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/9/1/73 |
| _version_ | 1818807612655796224 |
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| author | Tanaka Yoshiya Kanazawa Tamotsu Osada Yoshio Shimizu Shoichi Arai Meiji |
| author_facet | Tanaka Yoshiya Kanazawa Tamotsu Osada Yoshio Shimizu Shoichi Arai Meiji |
| author_sort | Tanaka Yoshiya |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Azithromycin (AZM) is a macrolide antibiotic that displays an excellent safety profile even in children and pregnant women and has been shown to have anti-malarial activity against blood stage <it>Plasmodium falciparum</it>. This study evaluated the transmission-blocking effect of AZM using a rodent malaria model.</p> <p>Methods</p> <p>AZM-treated mice infected with <it>Plasmodium berghei </it>were exposed to <it>Anopheles stephensi </it>mosquitoes, followed by the observation of parasite development at different phases in the mosquito, i.e., ookinetes in the midgut, oocysts on the midgut, and sporozoites in the midgut and salivary glands. Furthermore, to evaluate the effect on organelle replication of each stage, quantitative real-time PCR analysis was performed.</p> <p>Results</p> <p>The inhibitory effect of AZM was noticeable in both gametocyte-ookinete transformation in the midgut and sporozoite production in the oocyst, while the latter was most remarkable among all the developmental phases examined. Real-time PCR analysis revealed that AZM suppressed apicoplast replication at the period of sporozoite production in oocysts.</p> <p>Conclusions</p> <p>AZM inhibits parasite development in the mosquito stage, probably through the same mechanism as in the liver and blood stages. Such a multi-targeting anti-malarial, along with its safety, would be ideal for mass drug administration in malaria control programmes.</p> |
| first_indexed | 2024-12-18T19:28:26Z |
| format | Article |
| id | doaj.art-d989487f4e584f90840932987d62564a |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-18T19:28:26Z |
| publishDate | 2010-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-d989487f4e584f90840932987d62564a2022-12-21T20:55:48ZengBMCMalaria Journal1475-28752010-03-01917310.1186/1475-2875-9-73Suppressive effect of azithromycin on <it>Plasmodium berghei </it>mosquito stage development and apicoplast replicationTanaka YoshiyaKanazawa TamotsuOsada YoshioShimizu ShoichiArai Meiji<p>Abstract</p> <p>Background</p> <p>Azithromycin (AZM) is a macrolide antibiotic that displays an excellent safety profile even in children and pregnant women and has been shown to have anti-malarial activity against blood stage <it>Plasmodium falciparum</it>. This study evaluated the transmission-blocking effect of AZM using a rodent malaria model.</p> <p>Methods</p> <p>AZM-treated mice infected with <it>Plasmodium berghei </it>were exposed to <it>Anopheles stephensi </it>mosquitoes, followed by the observation of parasite development at different phases in the mosquito, i.e., ookinetes in the midgut, oocysts on the midgut, and sporozoites in the midgut and salivary glands. Furthermore, to evaluate the effect on organelle replication of each stage, quantitative real-time PCR analysis was performed.</p> <p>Results</p> <p>The inhibitory effect of AZM was noticeable in both gametocyte-ookinete transformation in the midgut and sporozoite production in the oocyst, while the latter was most remarkable among all the developmental phases examined. Real-time PCR analysis revealed that AZM suppressed apicoplast replication at the period of sporozoite production in oocysts.</p> <p>Conclusions</p> <p>AZM inhibits parasite development in the mosquito stage, probably through the same mechanism as in the liver and blood stages. Such a multi-targeting anti-malarial, along with its safety, would be ideal for mass drug administration in malaria control programmes.</p>http://www.malariajournal.com/content/9/1/73 |
| spellingShingle | Tanaka Yoshiya Kanazawa Tamotsu Osada Yoshio Shimizu Shoichi Arai Meiji Suppressive effect of azithromycin on <it>Plasmodium berghei </it>mosquito stage development and apicoplast replication Malaria Journal |
| title | Suppressive effect of azithromycin on <it>Plasmodium berghei </it>mosquito stage development and apicoplast replication |
| title_full | Suppressive effect of azithromycin on <it>Plasmodium berghei </it>mosquito stage development and apicoplast replication |
| title_fullStr | Suppressive effect of azithromycin on <it>Plasmodium berghei </it>mosquito stage development and apicoplast replication |
| title_full_unstemmed | Suppressive effect of azithromycin on <it>Plasmodium berghei </it>mosquito stage development and apicoplast replication |
| title_short | Suppressive effect of azithromycin on <it>Plasmodium berghei </it>mosquito stage development and apicoplast replication |
| title_sort | suppressive effect of azithromycin on it plasmodium berghei it mosquito stage development and apicoplast replication |
| url | http://www.malariajournal.com/content/9/1/73 |
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