PROPOLIS LOADED POLYVINYL ALCOHOL ATTENUATES CCL4 INDUCED HEPATIC FIBROSIS VIA MODULATION OF LET-7B/TGF-Β/SMAD SIGNALING PATHWAY
Chronic liver disorders are a serious global health issue due to their widespread incidence. Nephropathy described the deterioration of kidney function. Safe drug delivery by nanoparticles is a rapidly developing field with promising applications in the treatment of a wide variety of diseases. The...
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| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2023-01-01
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| Series: | Slovenian Veterinary Research |
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| Online Access: | https://www.slovetres.si/index.php/SVR/article/view/1568 |
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| author | Saydat Saad Doaa M. Abdelfatah Aya M. El-Sobky Tarek Khamis |
| author_facet | Saydat Saad Doaa M. Abdelfatah Aya M. El-Sobky Tarek Khamis |
| author_sort | Saydat Saad |
| collection | DOAJ |
| description |
Chronic liver disorders are a serious global health issue due to their widespread incidence. Nephropathy described the deterioration of kidney function. Safe drug delivery by nanoparticles is a rapidly developing field with promising applications in the treatment of a wide variety of diseases. The current study aimed to evaluate the use of propolis nanoparticles for managing carbon tetrachloride (CCl4)-induced hepato-nephropathy on rats. Seventy adult males Spargue Dawley rats were allocated into 7 equal groups 10 rats of each. Control, CCl4, CCl4 +Silymarin, CCl4 + propolis, CCl4+Nanopropolis, CCl4 +Silymarin +Propolis, CCl4 +Silymarin +Nanopropolis. Hepato- nephropathy was induced with oral administration of CCl4 dissolved in olive oil at dose of (1gm/kg) for 4 weeks. Silymarin, propolis and nanopropolis were orally administrated at a dose of (200mg /kg), (100 mg/kg) and (30 mg /kg) respectively for 4 weeks post hepato-nephropathy onset. Biochemical, molecular analysis, histological assessment of liver and kidney and serum oxidative stress were done. CCl4 caused a marked deterioration in biochemical, oxidative stress markers (MDA, TAC, CAT), serum TNF-α, IgM, molecular markers (SMAD-2, SMAD-3, SMAD-7, MMP-9, Desmin, TGF-β1, and let-7b), and the histopathological pictures of both liver and kidney. The above-mentioned parameters were restored with administration of silymarim + Nano-propolis, silymarin + propolis, silymarin, Nano-propolis, and propolis in order. Based on the previous findings we could speculated that combined therapy of nano-propolis and silymarin could be implicated in managing hepato-nepheropathy since it improves both liver and kideny function by targeting let-7b/TGF-β/Smad Pathway.
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| first_indexed | 2024-04-24T14:02:41Z |
| format | Article |
| id | doaj.art-d9898aa084f64b87bbf9a6aac995f625 |
| institution | Directory Open Access Journal |
| issn | 1580-4003 2385-8761 |
| language | English |
| last_indexed | 2024-04-24T14:02:41Z |
| publishDate | 2023-01-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Slovenian Veterinary Research |
| spelling | doaj.art-d9898aa084f64b87bbf9a6aac995f6252024-04-03T12:36:50ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Slovenian Veterinary Research1580-40032385-87612023-01-016025-Suppl10.26873/SVR-1568-2022PROPOLIS LOADED POLYVINYL ALCOHOL ATTENUATES CCL4 INDUCED HEPATIC FIBROSIS VIA MODULATION OF LET-7B/TGF-Β/SMAD SIGNALING PATHWAYSaydat Saad0Doaa M. Abdelfatah1Aya M. El-Sobky2Tarek Khamis3Biochemistry department Faculty of veterinary medicine, Zagazig University, 44519 Zagazig, EgyptBiochemistry department Faculty of veterinary medicine, Zagazig University, 44519 Zagazig, EgyptBiochemistry department Faculty of veterinary medicine, Zagazig University, 44519 Zagazig, Egypt, Corresponding author, E-mail: ayaelsobky93@yahoo.comPharmacology department, Faculty of veterinary medicine, Zagazig University, 44519 Zagazig, Egypt Chronic liver disorders are a serious global health issue due to their widespread incidence. Nephropathy described the deterioration of kidney function. Safe drug delivery by nanoparticles is a rapidly developing field with promising applications in the treatment of a wide variety of diseases. The current study aimed to evaluate the use of propolis nanoparticles for managing carbon tetrachloride (CCl4)-induced hepato-nephropathy on rats. Seventy adult males Spargue Dawley rats were allocated into 7 equal groups 10 rats of each. Control, CCl4, CCl4 +Silymarin, CCl4 + propolis, CCl4+Nanopropolis, CCl4 +Silymarin +Propolis, CCl4 +Silymarin +Nanopropolis. Hepato- nephropathy was induced with oral administration of CCl4 dissolved in olive oil at dose of (1gm/kg) for 4 weeks. Silymarin, propolis and nanopropolis were orally administrated at a dose of (200mg /kg), (100 mg/kg) and (30 mg /kg) respectively for 4 weeks post hepato-nephropathy onset. Biochemical, molecular analysis, histological assessment of liver and kidney and serum oxidative stress were done. CCl4 caused a marked deterioration in biochemical, oxidative stress markers (MDA, TAC, CAT), serum TNF-α, IgM, molecular markers (SMAD-2, SMAD-3, SMAD-7, MMP-9, Desmin, TGF-β1, and let-7b), and the histopathological pictures of both liver and kidney. The above-mentioned parameters were restored with administration of silymarim + Nano-propolis, silymarin + propolis, silymarin, Nano-propolis, and propolis in order. Based on the previous findings we could speculated that combined therapy of nano-propolis and silymarin could be implicated in managing hepato-nepheropathy since it improves both liver and kideny function by targeting let-7b/TGF-β/Smad Pathway. https://www.slovetres.si/index.php/SVR/article/view/1568gene expressionhepatic fibrosisnanoparticlesnephropathypropolisCCl4 |
| spellingShingle | Saydat Saad Doaa M. Abdelfatah Aya M. El-Sobky Tarek Khamis PROPOLIS LOADED POLYVINYL ALCOHOL ATTENUATES CCL4 INDUCED HEPATIC FIBROSIS VIA MODULATION OF LET-7B/TGF-Β/SMAD SIGNALING PATHWAY Slovenian Veterinary Research gene expression hepatic fibrosis nanoparticles nephropathy propolis CCl4 |
| title | PROPOLIS LOADED POLYVINYL ALCOHOL ATTENUATES CCL4 INDUCED HEPATIC FIBROSIS VIA MODULATION OF LET-7B/TGF-Β/SMAD SIGNALING PATHWAY |
| title_full | PROPOLIS LOADED POLYVINYL ALCOHOL ATTENUATES CCL4 INDUCED HEPATIC FIBROSIS VIA MODULATION OF LET-7B/TGF-Β/SMAD SIGNALING PATHWAY |
| title_fullStr | PROPOLIS LOADED POLYVINYL ALCOHOL ATTENUATES CCL4 INDUCED HEPATIC FIBROSIS VIA MODULATION OF LET-7B/TGF-Β/SMAD SIGNALING PATHWAY |
| title_full_unstemmed | PROPOLIS LOADED POLYVINYL ALCOHOL ATTENUATES CCL4 INDUCED HEPATIC FIBROSIS VIA MODULATION OF LET-7B/TGF-Β/SMAD SIGNALING PATHWAY |
| title_short | PROPOLIS LOADED POLYVINYL ALCOHOL ATTENUATES CCL4 INDUCED HEPATIC FIBROSIS VIA MODULATION OF LET-7B/TGF-Β/SMAD SIGNALING PATHWAY |
| title_sort | propolis loaded polyvinyl alcohol attenuates ccl4 induced hepatic fibrosis via modulation of let 7b tgf β smad signaling pathway |
| topic | gene expression hepatic fibrosis nanoparticles nephropathy propolis CCl4 |
| url | https://www.slovetres.si/index.php/SVR/article/view/1568 |
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