A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan Composite
In this paper, a microconductometric sensor has been designed, based on a chitosan composite including alcohol dehydrogenase—and its cofactor—and gold nanoparticles, and was calibrated by differential measurements in the headspace of aqueous solutions of ethanol. The role of gold nanoparticles (GNPs...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/16/2316 |
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| author | Anis Madaci Patcharapan Suwannin Guy Raffin Marie Hangouet Marie Martin Hana Ferkous Abderrazak Bouzid Joan Bausells Abdelhamid Elaissari Abdelhamid Errachid Nicole Jaffrezic-Renault |
| author_facet | Anis Madaci Patcharapan Suwannin Guy Raffin Marie Hangouet Marie Martin Hana Ferkous Abderrazak Bouzid Joan Bausells Abdelhamid Elaissari Abdelhamid Errachid Nicole Jaffrezic-Renault |
| author_sort | Anis Madaci |
| collection | DOAJ |
| description | In this paper, a microconductometric sensor has been designed, based on a chitosan composite including alcohol dehydrogenase—and its cofactor—and gold nanoparticles, and was calibrated by differential measurements in the headspace of aqueous solutions of ethanol. The role of gold nanoparticles (GNPs) was crucial in improving the analytical performance of the ethanol sensor in terms of response time, sensitivity, selectivity, and reproducibility. The response time was reduced to 10 s, compared to 21 s without GNPs. The sensitivity was 416 µS/cm (<i>v</i>/<i>v</i>%)<sup>−1</sup> which is 11.3 times higher than without GNPs. The selectivity factor versus methanol was 8.3, three times higher than without GNPs. The relative standard deviation (RSD) obtained with the same sensor was 2%, whereas it was found to be 12% without GNPs. When the air from the operator’s mouth was analyzed just after rinsing with an antiseptic mouthwash, the ethanol content was very high (3.5 <i>v</i>/<i>v</i>%). The background level was reached only after rinsing with water. |
| first_indexed | 2024-03-10T23:42:00Z |
| format | Article |
| id | doaj.art-d98d0e9f365742958b9b7b49caf32434 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T23:42:00Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-d98d0e9f365742958b9b7b49caf324342023-11-19T02:27:13ZengMDPI AGNanomaterials2079-49912023-08-011316231610.3390/nano13162316A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan CompositeAnis Madaci0Patcharapan Suwannin1Guy Raffin2Marie Hangouet3Marie Martin4Hana Ferkous5Abderrazak Bouzid6Joan Bausells7Abdelhamid Elaissari8Abdelhamid Errachid9Nicole Jaffrezic-Renault10Institute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, FranceInstitute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, FranceInstitute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, FranceInstitute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, FranceInstitute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, FranceLaboratory of Mechanical Engineering and Materials, Faculty of Technology, University of Skikda, Skikda 21000, AlgeriaLaboratory of Materials and Electronics Systems, University El-Bachir El-Ibrahimi Bordj Bou Arreridj, Bordj Bou Arreridj 34000, AlgeriaEl Consejo Superior de Investigaciones Científicas (CSIC), Centro Nacional de Microelectrónica (CNM), Institut de Microelectrònica de Barcelona (IMB), Campus UAB, 08193 Barcelona, SpainInstitute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, FranceInstitute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, FranceInstitute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, FranceIn this paper, a microconductometric sensor has been designed, based on a chitosan composite including alcohol dehydrogenase—and its cofactor—and gold nanoparticles, and was calibrated by differential measurements in the headspace of aqueous solutions of ethanol. The role of gold nanoparticles (GNPs) was crucial in improving the analytical performance of the ethanol sensor in terms of response time, sensitivity, selectivity, and reproducibility. The response time was reduced to 10 s, compared to 21 s without GNPs. The sensitivity was 416 µS/cm (<i>v</i>/<i>v</i>%)<sup>−1</sup> which is 11.3 times higher than without GNPs. The selectivity factor versus methanol was 8.3, three times higher than without GNPs. The relative standard deviation (RSD) obtained with the same sensor was 2%, whereas it was found to be 12% without GNPs. When the air from the operator’s mouth was analyzed just after rinsing with an antiseptic mouthwash, the ethanol content was very high (3.5 <i>v</i>/<i>v</i>%). The background level was reached only after rinsing with water.https://www.mdpi.com/2079-4991/13/16/2316ethanolmouthwashalcohol dehydrogenasechitosanconductometry |
| spellingShingle | Anis Madaci Patcharapan Suwannin Guy Raffin Marie Hangouet Marie Martin Hana Ferkous Abderrazak Bouzid Joan Bausells Abdelhamid Elaissari Abdelhamid Errachid Nicole Jaffrezic-Renault A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan Composite Nanomaterials ethanol mouthwash alcohol dehydrogenase chitosan conductometry |
| title | A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan Composite |
| title_full | A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan Composite |
| title_fullStr | A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan Composite |
| title_full_unstemmed | A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan Composite |
| title_short | A Sensitive Micro Conductometric Ethanol Sensor Based on an Alcohol Dehydrogenase-Gold Nanoparticle Chitosan Composite |
| title_sort | sensitive micro conductometric ethanol sensor based on an alcohol dehydrogenase gold nanoparticle chitosan composite |
| topic | ethanol mouthwash alcohol dehydrogenase chitosan conductometry |
| url | https://www.mdpi.com/2079-4991/13/16/2316 |
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