Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana
IntroductionBipolaris sorokiniana is the popular pathogenic fungi fungus which lead to common root rot and leaf spot on wheat. Generally, chemical fungicides are used to control diseases. However, the environmental pollution resulting from fungicides should not be ignored. It is important to study t...
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1149363/full |
| _version_ | 1797847526002393088 |
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| author | Pengyu Luan Pengyu Luan Yanjie Yi Yanjie Yi Yifan Huang Yifan Huang Liuqing Cui Liuqing Cui Zhipeng Hou Zhipeng Hou Lijuan Zhu Lijuan Zhu Xiujuan Ren Xiujuan Ren Shao Jia Shao Jia Yang Liu Yang Liu |
| author_facet | Pengyu Luan Pengyu Luan Yanjie Yi Yanjie Yi Yifan Huang Yifan Huang Liuqing Cui Liuqing Cui Zhipeng Hou Zhipeng Hou Lijuan Zhu Lijuan Zhu Xiujuan Ren Xiujuan Ren Shao Jia Shao Jia Yang Liu Yang Liu |
| author_sort | Pengyu Luan |
| collection | DOAJ |
| description | IntroductionBipolaris sorokiniana is the popular pathogenic fungi fungus which lead to common root rot and leaf spot on wheat. Generally, chemical fungicides are used to control diseases. However, the environmental pollution resulting from fungicides should not be ignored. It is important to study the mode of antagonistic action between biocontrol microbes and plant pathogens to design efficient biocontrol strategies.ResultsAn antagonistic bacterium DB2 was isolated and identified as Bacillus amyloliquefaciens. The inhibition rate of cell-free culture filtrate (CF, 20%, v/v) of DB2 against B. sorokiniana reached 92.67%. Light microscopy and scanning electron microscopy (SEM) showed that the CF significantly altered the mycelial morphology of B. sorokiniana and disrupted cellular integrity. Fluorescence microscopy showed that culture filtrate destroyed mycelial cell membrane integrity, decreased the mitochondrial transmembrane potential, induced reactive oxygen species (ROS) accumulation, and nuclear damage which caused cell death in B. sorokiniana. Moreover, the strain exhibited considerable production of protease and amylase, and showed a significant siderophore and indole-3-acetic acid (IAA) production. In the detached leaves and potted plants control assay, B. amyloliquefacien DB2 had remarkable inhibition activity against B. sorokiniana and the pot control efficacy was 75.22%. Furthermore, DB2 suspension had a significant promotion for wheat seedlings growth.ConclusionB. amyloliquefaciens DB2 can be taken as a potential biocontrol agent to inhibit B. sorokiniana on wheat and promote wheat growth. |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T18:12:52Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-d98d687ce8e640f69e836a7bfc0c99e82023-04-13T14:15:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11493631149363Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokinianaPengyu Luan0Pengyu Luan1Yanjie Yi2Yanjie Yi3Yifan Huang4Yifan Huang5Liuqing Cui6Liuqing Cui7Zhipeng Hou8Zhipeng Hou9Lijuan Zhu10Lijuan Zhu11Xiujuan Ren12Xiujuan Ren13Shao Jia14Shao Jia15Yang Liu16Yang Liu17School of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaKey Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaKey Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaKey Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaKey Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaKey Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaKey Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaKey Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaKey Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, ChinaSchool of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaKey Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, ChinaIntroductionBipolaris sorokiniana is the popular pathogenic fungi fungus which lead to common root rot and leaf spot on wheat. Generally, chemical fungicides are used to control diseases. However, the environmental pollution resulting from fungicides should not be ignored. It is important to study the mode of antagonistic action between biocontrol microbes and plant pathogens to design efficient biocontrol strategies.ResultsAn antagonistic bacterium DB2 was isolated and identified as Bacillus amyloliquefaciens. The inhibition rate of cell-free culture filtrate (CF, 20%, v/v) of DB2 against B. sorokiniana reached 92.67%. Light microscopy and scanning electron microscopy (SEM) showed that the CF significantly altered the mycelial morphology of B. sorokiniana and disrupted cellular integrity. Fluorescence microscopy showed that culture filtrate destroyed mycelial cell membrane integrity, decreased the mitochondrial transmembrane potential, induced reactive oxygen species (ROS) accumulation, and nuclear damage which caused cell death in B. sorokiniana. Moreover, the strain exhibited considerable production of protease and amylase, and showed a significant siderophore and indole-3-acetic acid (IAA) production. In the detached leaves and potted plants control assay, B. amyloliquefacien DB2 had remarkable inhibition activity against B. sorokiniana and the pot control efficacy was 75.22%. Furthermore, DB2 suspension had a significant promotion for wheat seedlings growth.ConclusionB. amyloliquefaciens DB2 can be taken as a potential biocontrol agent to inhibit B. sorokiniana on wheat and promote wheat growth.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1149363/fullBacillus amyloliquefaciens DB2Bipolaris sorokinianabiocontrol potentialantifungal mechanismplant growth-promotion |
| spellingShingle | Pengyu Luan Pengyu Luan Yanjie Yi Yanjie Yi Yifan Huang Yifan Huang Liuqing Cui Liuqing Cui Zhipeng Hou Zhipeng Hou Lijuan Zhu Lijuan Zhu Xiujuan Ren Xiujuan Ren Shao Jia Shao Jia Yang Liu Yang Liu Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana Frontiers in Microbiology Bacillus amyloliquefaciens DB2 Bipolaris sorokiniana biocontrol potential antifungal mechanism plant growth-promotion |
| title | Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana |
| title_full | Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana |
| title_fullStr | Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana |
| title_full_unstemmed | Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana |
| title_short | Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana |
| title_sort | biocontrol potential and action mechanism of bacillus amyloliquefaciens db2 on bipolaris sorokiniana |
| topic | Bacillus amyloliquefaciens DB2 Bipolaris sorokiniana biocontrol potential antifungal mechanism plant growth-promotion |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1149363/full |
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