Detection of maternal transmission of resistant Gram-negative bacteria in a Cambodian hospital setting
Infection with Extended-spectrum beta-lactamase -producing Enterobacterales (ESBL-E) is common in infants and leads to increased intensive care unit admission and mortality, but the role of maternal transmission in colonization of infants is unclear. Using paired isolates from 50 pairs of mothers an...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158056/full |
| _version_ | 1797847256213225472 |
|---|---|
| author | Chalita Chomkatekaew Janjira Thaipadungpanit Janjira Thaipadungpanit Pasco Hearn Sona Soeng Sreymom Pol Leakhena Neou Jill Hopkins Jill Hopkins Paul Turner Paul Turner Elizabeth M. Batty Elizabeth M. Batty |
| author_facet | Chalita Chomkatekaew Janjira Thaipadungpanit Janjira Thaipadungpanit Pasco Hearn Sona Soeng Sreymom Pol Leakhena Neou Jill Hopkins Jill Hopkins Paul Turner Paul Turner Elizabeth M. Batty Elizabeth M. Batty |
| author_sort | Chalita Chomkatekaew |
| collection | DOAJ |
| description | Infection with Extended-spectrum beta-lactamase -producing Enterobacterales (ESBL-E) is common in infants and leads to increased intensive care unit admission and mortality, but the role of maternal transmission in colonization of infants is unclear. Using paired isolates from 50 pairs of mothers and neonates admitted to a Cambodian hospital, we investigated antimicrobial resistance in Escherichia coli and Klebsiella pneumoniae using whole genome sequencing. We detected a wide variety of ESBL-E genes present in this population along with high levels of multidrug resistance. From 21 pairs where the same organism was present in both mother and neonate, we identified eight pairs with identical or near-identical isolates from both individuals suggestive of transmission at or around birth, including a pair with transmission of multiple strains. We found no evidence for transmission of plasmids only from mother to infant. This suggests vertical transmission outside hospitals as a common cause of ESBL-E colonization in neonates. |
| first_indexed | 2024-04-09T18:09:28Z |
| format | Article |
| id | doaj.art-d98e4977d9ac4f9d8d6720c4b66124b7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T18:09:28Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d98e4977d9ac4f9d8d6720c4b66124b72023-04-14T04:26:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11580561158056Detection of maternal transmission of resistant Gram-negative bacteria in a Cambodian hospital settingChalita Chomkatekaew0Janjira Thaipadungpanit1Janjira Thaipadungpanit2Pasco Hearn3Sona Soeng4Sreymom Pol5Leakhena Neou6Jill Hopkins7Jill Hopkins8Paul Turner9Paul Turner10Elizabeth M. Batty11Elizabeth M. Batty12Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandMahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandDepartment of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandCambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, CambodiaCambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, CambodiaCambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, CambodiaNeonatal Unit, Angkor Hospital for Children, Siem Reap, CambodiaCambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, CambodiaCenter for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United KingdomCambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, CambodiaCenter for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United KingdomMahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandCenter for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United KingdomInfection with Extended-spectrum beta-lactamase -producing Enterobacterales (ESBL-E) is common in infants and leads to increased intensive care unit admission and mortality, but the role of maternal transmission in colonization of infants is unclear. Using paired isolates from 50 pairs of mothers and neonates admitted to a Cambodian hospital, we investigated antimicrobial resistance in Escherichia coli and Klebsiella pneumoniae using whole genome sequencing. We detected a wide variety of ESBL-E genes present in this population along with high levels of multidrug resistance. From 21 pairs where the same organism was present in both mother and neonate, we identified eight pairs with identical or near-identical isolates from both individuals suggestive of transmission at or around birth, including a pair with transmission of multiple strains. We found no evidence for transmission of plasmids only from mother to infant. This suggests vertical transmission outside hospitals as a common cause of ESBL-E colonization in neonates.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158056/fullantimicrobial resistancebacterial transmissionmicrobiologyEscherichia coliKlebsiella pneumoniaematernal transmission |
| spellingShingle | Chalita Chomkatekaew Janjira Thaipadungpanit Janjira Thaipadungpanit Pasco Hearn Sona Soeng Sreymom Pol Leakhena Neou Jill Hopkins Jill Hopkins Paul Turner Paul Turner Elizabeth M. Batty Elizabeth M. Batty Detection of maternal transmission of resistant Gram-negative bacteria in a Cambodian hospital setting Frontiers in Microbiology antimicrobial resistance bacterial transmission microbiology Escherichia coli Klebsiella pneumoniae maternal transmission |
| title | Detection of maternal transmission of resistant Gram-negative bacteria in a Cambodian hospital setting |
| title_full | Detection of maternal transmission of resistant Gram-negative bacteria in a Cambodian hospital setting |
| title_fullStr | Detection of maternal transmission of resistant Gram-negative bacteria in a Cambodian hospital setting |
| title_full_unstemmed | Detection of maternal transmission of resistant Gram-negative bacteria in a Cambodian hospital setting |
| title_short | Detection of maternal transmission of resistant Gram-negative bacteria in a Cambodian hospital setting |
| title_sort | detection of maternal transmission of resistant gram negative bacteria in a cambodian hospital setting |
| topic | antimicrobial resistance bacterial transmission microbiology Escherichia coli Klebsiella pneumoniae maternal transmission |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158056/full |
| work_keys_str_mv | AT chalitachomkatekaew detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT janjirathaipadungpanit detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT janjirathaipadungpanit detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT pascohearn detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT sonasoeng detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT sreymompol detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT leakhenaneou detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT jillhopkins detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT jillhopkins detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT paulturner detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT paulturner detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT elizabethmbatty detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting AT elizabethmbatty detectionofmaternaltransmissionofresistantgramnegativebacteriainacambodianhospitalsetting |