<it>In vitro </it>effects of 2-methoxyestradiol-bis-sulphamate on reactive oxygen species and possible apoptosis induction in a breast adenocarcinoma cell line
<p>Abstract</p> <p>Background</p> <p>In the search for anticancer agents, a promising 17-β-estradiol metabolite, 2-methoxyestradiol (2ME2) was found that exerts antiproliferative <it>in vitro </it>and <it>in vivo </it>activity. Since 2ME2 has lim...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2011-12-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | http://www.cancerci.com/content/11/1/43 |
| _version_ | 1811311755986468864 |
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| author | Visagie Michelle H Joubert Anna M |
| author_facet | Visagie Michelle H Joubert Anna M |
| author_sort | Visagie Michelle H |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In the search for anticancer agents, a promising 17-β-estradiol metabolite, 2-methoxyestradiol (2ME2) was found that exerts antiproliferative <it>in vitro </it>and <it>in vivo </it>activity. Since 2ME2 has limited biological accessibility and rapid metabolic degradation, the purpose of this study was to investigate the <it>in vitro </it>influence exerted by an analogue of 2ME2 namely 2-methoxyestradiol-bis-sulphamate (2MEBM) in a breast adenocarcinoma cell line (MCF-7).</p> <p>Methods</p> <p>This was conducted by investigating 2MEBM's <it>in vitro </it>influence on cell cycle progression, mitochondrial membrane potential and possible production of reactive oxygen species (ROS) generation. <it>In vitro </it>effects of 2MEBM on cell cycle progression was demonstrated by means of flow cytometry using propidium iodide. Hydrogen peroxide and superoxide production was investigated using 2,7-dichlorofluorescein diacetate and hydroethidine, respectively. The probable reduction in the mitochondrial membrane potential was demonstrated using a MitoCapture™ kit.</p> <p>Results</p> <p>Cell cycle progression revealed the presence of a sub-G<sub>1 </sub>apoptotic peak. Reduction of mitochondrial membrane potential after exposure to 2MEBM was demonstrated and an increase in ROS production was also observed.</p> <p>Conclusion</p> <p>This study verified that 2MEBM exposure resulted in apoptosis induction, increased ROS production and reduced mitochondrial membrane potential in a tumorigenic breast epithelial cell line. Data obtained from this project contributes to the unravelling of the <it>in vitro </it>signal transduction of 2MEBM in tumorigenic cell lines.</p> |
| first_indexed | 2024-04-13T10:24:23Z |
| format | Article |
| id | doaj.art-d99160b2171a405f9976e6783f56f313 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-04-13T10:24:23Z |
| publishDate | 2011-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-d99160b2171a405f9976e6783f56f3132022-12-22T02:50:23ZengBMCCancer Cell International1475-28672011-12-011114310.1186/1475-2867-11-43<it>In vitro </it>effects of 2-methoxyestradiol-bis-sulphamate on reactive oxygen species and possible apoptosis induction in a breast adenocarcinoma cell lineVisagie Michelle HJoubert Anna M<p>Abstract</p> <p>Background</p> <p>In the search for anticancer agents, a promising 17-β-estradiol metabolite, 2-methoxyestradiol (2ME2) was found that exerts antiproliferative <it>in vitro </it>and <it>in vivo </it>activity. Since 2ME2 has limited biological accessibility and rapid metabolic degradation, the purpose of this study was to investigate the <it>in vitro </it>influence exerted by an analogue of 2ME2 namely 2-methoxyestradiol-bis-sulphamate (2MEBM) in a breast adenocarcinoma cell line (MCF-7).</p> <p>Methods</p> <p>This was conducted by investigating 2MEBM's <it>in vitro </it>influence on cell cycle progression, mitochondrial membrane potential and possible production of reactive oxygen species (ROS) generation. <it>In vitro </it>effects of 2MEBM on cell cycle progression was demonstrated by means of flow cytometry using propidium iodide. Hydrogen peroxide and superoxide production was investigated using 2,7-dichlorofluorescein diacetate and hydroethidine, respectively. The probable reduction in the mitochondrial membrane potential was demonstrated using a MitoCapture™ kit.</p> <p>Results</p> <p>Cell cycle progression revealed the presence of a sub-G<sub>1 </sub>apoptotic peak. Reduction of mitochondrial membrane potential after exposure to 2MEBM was demonstrated and an increase in ROS production was also observed.</p> <p>Conclusion</p> <p>This study verified that 2MEBM exposure resulted in apoptosis induction, increased ROS production and reduced mitochondrial membrane potential in a tumorigenic breast epithelial cell line. Data obtained from this project contributes to the unravelling of the <it>in vitro </it>signal transduction of 2MEBM in tumorigenic cell lines.</p>http://www.cancerci.com/content/11/1/432-methoxyestradiol-bis-sulphamatecell cycle progressionreactive oxygen speciesmitochondrial integrityapoptosisautophagy |
| spellingShingle | Visagie Michelle H Joubert Anna M <it>In vitro </it>effects of 2-methoxyestradiol-bis-sulphamate on reactive oxygen species and possible apoptosis induction in a breast adenocarcinoma cell line Cancer Cell International 2-methoxyestradiol-bis-sulphamate cell cycle progression reactive oxygen species mitochondrial integrity apoptosis autophagy |
| title | <it>In vitro </it>effects of 2-methoxyestradiol-bis-sulphamate on reactive oxygen species and possible apoptosis induction in a breast adenocarcinoma cell line |
| title_full | <it>In vitro </it>effects of 2-methoxyestradiol-bis-sulphamate on reactive oxygen species and possible apoptosis induction in a breast adenocarcinoma cell line |
| title_fullStr | <it>In vitro </it>effects of 2-methoxyestradiol-bis-sulphamate on reactive oxygen species and possible apoptosis induction in a breast adenocarcinoma cell line |
| title_full_unstemmed | <it>In vitro </it>effects of 2-methoxyestradiol-bis-sulphamate on reactive oxygen species and possible apoptosis induction in a breast adenocarcinoma cell line |
| title_short | <it>In vitro </it>effects of 2-methoxyestradiol-bis-sulphamate on reactive oxygen species and possible apoptosis induction in a breast adenocarcinoma cell line |
| title_sort | it in vitro it effects of 2 methoxyestradiol bis sulphamate on reactive oxygen species and possible apoptosis induction in a breast adenocarcinoma cell line |
| topic | 2-methoxyestradiol-bis-sulphamate cell cycle progression reactive oxygen species mitochondrial integrity apoptosis autophagy |
| url | http://www.cancerci.com/content/11/1/43 |
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