Antioxidant and antiradical activity of extracts of phenolic compounds from red bean
Extract of red bean seeds was prepared using 80% acetone. Crude extract was applied onto a Sephadex LH-20 column. Fraction I of low molecular weight phenolic compounds was eluted from the column by ethanol. Fraction II of tannins was eluted from the column using water-acetone (1:1, v/v) as a mobile...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2004-12-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200410-0039_antioxidant-and-antiradical-activity-of-extracts-of-phenolic-compounds-from-red-bean.php |
| _version_ | 1797899911680753664 |
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| author | R. Amarowicz A. Troszyńska |
| author_facet | R. Amarowicz A. Troszyńska |
| author_sort | R. Amarowicz |
| collection | DOAJ |
| description | Extract of red bean seeds was prepared using 80% acetone. Crude extract was applied onto a Sephadex LH-20 column. Fraction I of low molecular weight phenolic compounds was eluted from the column by ethanol. Fraction II of tannins was eluted from the column using water-acetone (1:1, v/v) as a mobile phase. Phenolic compounds present in the extracts and in the fractions I and II showed antioxidant and antiradical properties investigated using a β-carotene-linoleate model system, and DPPH radical scavenging activity. Crude extract showed the highest antioxidant activity in a β-carotene-linoleate model system. However, tannins fraction was the strongest scavengers of DPPH radical. The content of total phenolics in the fraction II was found the highest (139 mg/g). |
| first_indexed | 2024-04-10T08:36:25Z |
| format | Article |
| id | doaj.art-d99456d48a294987b10b3e67a07ea307 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:36:25Z |
| publishDate | 2004-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-d99456d48a294987b10b3e67a07ea3072023-02-23T03:26:55ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172004-12-0122Special IssueS206S208cjf-200410-0039Antioxidant and antiradical activity of extracts of phenolic compounds from red beanR. AmarowiczA. TroszyńskaExtract of red bean seeds was prepared using 80% acetone. Crude extract was applied onto a Sephadex LH-20 column. Fraction I of low molecular weight phenolic compounds was eluted from the column by ethanol. Fraction II of tannins was eluted from the column using water-acetone (1:1, v/v) as a mobile phase. Phenolic compounds present in the extracts and in the fractions I and II showed antioxidant and antiradical properties investigated using a β-carotene-linoleate model system, and DPPH radical scavenging activity. Crude extract showed the highest antioxidant activity in a β-carotene-linoleate model system. However, tannins fraction was the strongest scavengers of DPPH radical. The content of total phenolics in the fraction II was found the highest (139 mg/g).https://cjfs.agriculturejournals.cz/artkey/cjf-200410-0039_antioxidant-and-antiradical-activity-of-extracts-of-phenolic-compounds-from-red-bean.phpred beanphenolic compoundsantioxidant activitydpph scavenging activitycolumn chromatography |
| spellingShingle | R. Amarowicz A. Troszyńska Antioxidant and antiradical activity of extracts of phenolic compounds from red bean Czech Journal of Food Sciences red bean phenolic compounds antioxidant activity dpph scavenging activity column chromatography |
| title | Antioxidant and antiradical activity of extracts of phenolic compounds from red bean |
| title_full | Antioxidant and antiradical activity of extracts of phenolic compounds from red bean |
| title_fullStr | Antioxidant and antiradical activity of extracts of phenolic compounds from red bean |
| title_full_unstemmed | Antioxidant and antiradical activity of extracts of phenolic compounds from red bean |
| title_short | Antioxidant and antiradical activity of extracts of phenolic compounds from red bean |
| title_sort | antioxidant and antiradical activity of extracts of phenolic compounds from red bean |
| topic | red bean phenolic compounds antioxidant activity dpph scavenging activity column chromatography |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200410-0039_antioxidant-and-antiradical-activity-of-extracts-of-phenolic-compounds-from-red-bean.php |
| work_keys_str_mv | AT ramarowicz antioxidantandantiradicalactivityofextractsofphenoliccompoundsfromredbean AT atroszynska antioxidantandantiradicalactivityofextractsofphenoliccompoundsfromredbean |