A Comparative Metabolomic Analysis Reveals the Nutritional and Therapeutic Potential of Grains of the Traditional Rice Variety Mappillai Samba
Rice (<i>Oryza sativa</i> L.) is the staple food of the majority of the population, particularly in Asia and Africa. Enriching rice with nutritional and therapeutic contents can improve its benefits for patients with lifestyle disorders. This study aimed to profile the phytochemical cont...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/4/543 |
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| author | Veera Ranjani Rajagopalan Sudha Manickam Raveendran Muthurajan |
| author_facet | Veera Ranjani Rajagopalan Sudha Manickam Raveendran Muthurajan |
| author_sort | Veera Ranjani Rajagopalan |
| collection | DOAJ |
| description | Rice (<i>Oryza sativa</i> L.) is the staple food of the majority of the population, particularly in Asia and Africa. Enriching rice with nutritional and therapeutic contents can improve its benefits for patients with lifestyle disorders. This study aimed to profile the phytochemical contents of the therapeutically known traditional rice Mappillai Samba against white rice CBMAS 14065 using non-targeted gas chromatography–mass spectrometry (GC-MS/MS). An analysis of the data using a mass spectrometry–data independent analysis (MS-DIAL) and MetaboAnalyst identified 113 metabolites belonging to 21 different classes of metabolites. A partial least square-discriminant analysis (PLS-DA) revealed 43 variable importance in projection (VIP) metabolites. This study identified therapeutically important metabolites, including phenylpropanoids, phytosterols, flavonoids, and polyamines, in the grains of Mappillai Samba. Three significant metabolic pathways, viz., phenylpropanoid biosynthesis, ubiquinone and other terpenoid-quinone biosynthesis, and steroid biosynthesis, were responsible for the grain metabolome variation between CBMAS 14065 and Mappillai Samba. Overall, the results of this study unravelled the biochemical complexity of Mappillai Samba, paving the way for the genetic mapping of the therapeutic compound accumulation in rice and the development of similar therapeutic rice varieties through molecular breeding. |
| first_indexed | 2024-03-09T21:12:28Z |
| format | Article |
| id | doaj.art-d994929eb42242a2b67374394e3518f5 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T21:12:28Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d994929eb42242a2b67374394e3518f52023-11-23T21:43:07ZengMDPI AGPlants2223-77472022-02-0111454310.3390/plants11040543A Comparative Metabolomic Analysis Reveals the Nutritional and Therapeutic Potential of Grains of the Traditional Rice Variety Mappillai SambaVeera Ranjani Rajagopalan0Sudha Manickam1Raveendran Muthurajan2Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaDepartment of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaDepartment of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, IndiaRice (<i>Oryza sativa</i> L.) is the staple food of the majority of the population, particularly in Asia and Africa. Enriching rice with nutritional and therapeutic contents can improve its benefits for patients with lifestyle disorders. This study aimed to profile the phytochemical contents of the therapeutically known traditional rice Mappillai Samba against white rice CBMAS 14065 using non-targeted gas chromatography–mass spectrometry (GC-MS/MS). An analysis of the data using a mass spectrometry–data independent analysis (MS-DIAL) and MetaboAnalyst identified 113 metabolites belonging to 21 different classes of metabolites. A partial least square-discriminant analysis (PLS-DA) revealed 43 variable importance in projection (VIP) metabolites. This study identified therapeutically important metabolites, including phenylpropanoids, phytosterols, flavonoids, and polyamines, in the grains of Mappillai Samba. Three significant metabolic pathways, viz., phenylpropanoid biosynthesis, ubiquinone and other terpenoid-quinone biosynthesis, and steroid biosynthesis, were responsible for the grain metabolome variation between CBMAS 14065 and Mappillai Samba. Overall, the results of this study unravelled the biochemical complexity of Mappillai Samba, paving the way for the genetic mapping of the therapeutic compound accumulation in rice and the development of similar therapeutic rice varieties through molecular breeding.https://www.mdpi.com/2223-7747/11/4/543traditional riceMappillai Sambametabolomicstherapeutic compoundsphytosterolsantioxidants |
| spellingShingle | Veera Ranjani Rajagopalan Sudha Manickam Raveendran Muthurajan A Comparative Metabolomic Analysis Reveals the Nutritional and Therapeutic Potential of Grains of the Traditional Rice Variety Mappillai Samba Plants traditional rice Mappillai Samba metabolomics therapeutic compounds phytosterols antioxidants |
| title | A Comparative Metabolomic Analysis Reveals the Nutritional and Therapeutic Potential of Grains of the Traditional Rice Variety Mappillai Samba |
| title_full | A Comparative Metabolomic Analysis Reveals the Nutritional and Therapeutic Potential of Grains of the Traditional Rice Variety Mappillai Samba |
| title_fullStr | A Comparative Metabolomic Analysis Reveals the Nutritional and Therapeutic Potential of Grains of the Traditional Rice Variety Mappillai Samba |
| title_full_unstemmed | A Comparative Metabolomic Analysis Reveals the Nutritional and Therapeutic Potential of Grains of the Traditional Rice Variety Mappillai Samba |
| title_short | A Comparative Metabolomic Analysis Reveals the Nutritional and Therapeutic Potential of Grains of the Traditional Rice Variety Mappillai Samba |
| title_sort | comparative metabolomic analysis reveals the nutritional and therapeutic potential of grains of the traditional rice variety mappillai samba |
| topic | traditional rice Mappillai Samba metabolomics therapeutic compounds phytosterols antioxidants |
| url | https://www.mdpi.com/2223-7747/11/4/543 |
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