First molecular detection of influenza D virus in cattle from commercial farm in Nigeria, Sub-Saharan Africa
Objective: To carrry out molecular surveillance of influenza D virus (IDV) in cattle in Lagos, Nigeria. Methods: Prospective epidemiological investigation was initiated in a large commercial farm market where animals in open pens were reared, sold, and butchered under poor hygienic conditions withou...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | One Health Bulletin |
| Subjects: | |
| Online Access: | http://www.johb.info/article.asp?issn=2773-0344;year=2023;volume=3;issue=1;spage=19;epage=19;aulast=Anjorin |
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| author | Abdul-Azeez A Anjorin Gideon O Moronkeji Goodness O Temenu Omolade A Maiyegun Christopher O Fakorede Samuel O Ajoseh Wasiu O Salami Rebecca O Abegunrin Kehinde O Amisu Kabiru O Akinyemi |
| author_facet | Abdul-Azeez A Anjorin Gideon O Moronkeji Goodness O Temenu Omolade A Maiyegun Christopher O Fakorede Samuel O Ajoseh Wasiu O Salami Rebecca O Abegunrin Kehinde O Amisu Kabiru O Akinyemi |
| author_sort | Abdul-Azeez A Anjorin |
| collection | DOAJ |
| description | Objective: To carrry out molecular surveillance of influenza D virus (IDV) in cattle in Lagos, Nigeria.
Methods: Prospective epidemiological investigation was initiated in a large commercial farm market where animals in open pens were reared, sold, and butchered under poor hygienic conditions without adequate biosecurity measures. A total of 80 nasopharyngeal swabs were collected from cattle between October and November 2021. The samples were extracted using an RNA purification kit (NIMR). RNA extracts were amplified following a two-step PCR using FIREScript RT cDNA synthesis kit (Solis Biodyne, Estonia), followed by PCR OneTaq Quick-load 2X master-mix (NEB, UK) in a Rotor-Gene thermocycler (Qiagen, Germany). Amplicons were detected using a 1.5% Gel electrophoresis.
Results: In total, 32.5% (26/80) IDV was detected in cattle. Sick animals showed higher burden of IDV with 65.4% (17/26) than 34.6% (9/26) in a healthy population, including 88.2% (15/17) cattle with diarrhoea and 11.8% (2/17) with nausea having IDV. An incidence of 69.2% (18/26) by sex was recorded in bull, which was more than twice compared to 30.7% (8/26) in cow. Age prevalence showed 62.2% (18/26), the highest detection in cattle of four years old, followed by 23.1% (6/26) in five years old, while the lowest 7.7% (2/26) was recorded in three years old.
Conclusions: To the best of our knowledge, this study presents the first molecular detection of IDV in Nigeria and West Africa sub-region. It underscores the need for continuous surveillance of IDV at the animal-human interface. |
| first_indexed | 2024-03-08T05:11:36Z |
| format | Article |
| id | doaj.art-d994f9024cbd477cb7347aa7f2ece0ff |
| institution | Directory Open Access Journal |
| issn | 2773-0344 2773-0352 |
| language | English |
| last_indexed | 2024-03-08T05:11:36Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | One Health Bulletin |
| spelling | doaj.art-d994f9024cbd477cb7347aa7f2ece0ff2024-02-07T09:12:57ZengWolters Kluwer Medknow PublicationsOne Health Bulletin2773-03442773-03522023-01-0131191910.4103/2773-0344.387694First molecular detection of influenza D virus in cattle from commercial farm in Nigeria, Sub-Saharan AfricaAbdul-Azeez A AnjorinGideon O MoronkejiGoodness O TemenuOmolade A MaiyegunChristopher O FakoredeSamuel O AjosehWasiu O SalamiRebecca O AbegunrinKehinde O AmisuKabiru O AkinyemiObjective: To carrry out molecular surveillance of influenza D virus (IDV) in cattle in Lagos, Nigeria. Methods: Prospective epidemiological investigation was initiated in a large commercial farm market where animals in open pens were reared, sold, and butchered under poor hygienic conditions without adequate biosecurity measures. A total of 80 nasopharyngeal swabs were collected from cattle between October and November 2021. The samples were extracted using an RNA purification kit (NIMR). RNA extracts were amplified following a two-step PCR using FIREScript RT cDNA synthesis kit (Solis Biodyne, Estonia), followed by PCR OneTaq Quick-load 2X master-mix (NEB, UK) in a Rotor-Gene thermocycler (Qiagen, Germany). Amplicons were detected using a 1.5% Gel electrophoresis. Results: In total, 32.5% (26/80) IDV was detected in cattle. Sick animals showed higher burden of IDV with 65.4% (17/26) than 34.6% (9/26) in a healthy population, including 88.2% (15/17) cattle with diarrhoea and 11.8% (2/17) with nausea having IDV. An incidence of 69.2% (18/26) by sex was recorded in bull, which was more than twice compared to 30.7% (8/26) in cow. Age prevalence showed 62.2% (18/26), the highest detection in cattle of four years old, followed by 23.1% (6/26) in five years old, while the lowest 7.7% (2/26) was recorded in three years old. Conclusions: To the best of our knowledge, this study presents the first molecular detection of IDV in Nigeria and West Africa sub-region. It underscores the need for continuous surveillance of IDV at the animal-human interface.http://www.johb.info/article.asp?issn=2773-0344;year=2023;volume=3;issue=1;spage=19;epage=19;aulast=Anjorininfluenza d virus (idv); molecular surveillance; pcr; cattle; one health |
| spellingShingle | Abdul-Azeez A Anjorin Gideon O Moronkeji Goodness O Temenu Omolade A Maiyegun Christopher O Fakorede Samuel O Ajoseh Wasiu O Salami Rebecca O Abegunrin Kehinde O Amisu Kabiru O Akinyemi First molecular detection of influenza D virus in cattle from commercial farm in Nigeria, Sub-Saharan Africa One Health Bulletin influenza d virus (idv); molecular surveillance; pcr; cattle; one health |
| title | First molecular detection of influenza D virus in cattle from commercial farm in Nigeria, Sub-Saharan Africa |
| title_full | First molecular detection of influenza D virus in cattle from commercial farm in Nigeria, Sub-Saharan Africa |
| title_fullStr | First molecular detection of influenza D virus in cattle from commercial farm in Nigeria, Sub-Saharan Africa |
| title_full_unstemmed | First molecular detection of influenza D virus in cattle from commercial farm in Nigeria, Sub-Saharan Africa |
| title_short | First molecular detection of influenza D virus in cattle from commercial farm in Nigeria, Sub-Saharan Africa |
| title_sort | first molecular detection of influenza d virus in cattle from commercial farm in nigeria sub saharan africa |
| topic | influenza d virus (idv); molecular surveillance; pcr; cattle; one health |
| url | http://www.johb.info/article.asp?issn=2773-0344;year=2023;volume=3;issue=1;spage=19;epage=19;aulast=Anjorin |
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