MiR-486-3p and MiR-938—Important Inhibitors of Pacemaking Ion Channels and/or Markers of Immune Cells
The sinus node (SN) is the heart’s primary pacemaker and has a unique expression of pacemaking ion channels and immune cell markers. The role of microribonucleic acids (miRNAs) in control of ion channels and immune function of the sinus node is not well understood. We have recently shown that hsa-mi...
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MDPI AG
2021-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/23/11366 |
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| author | Abimbola J Aminu Maria Petkova Weixuan Chen Zeyuan Yin Vlad S Kuzmin Andrew J Atkinson Halina Dobrzynski |
| author_facet | Abimbola J Aminu Maria Petkova Weixuan Chen Zeyuan Yin Vlad S Kuzmin Andrew J Atkinson Halina Dobrzynski |
| author_sort | Abimbola J Aminu |
| collection | DOAJ |
| description | The sinus node (SN) is the heart’s primary pacemaker and has a unique expression of pacemaking ion channels and immune cell markers. The role of microribonucleic acids (miRNAs) in control of ion channels and immune function of the sinus node is not well understood. We have recently shown that hsa-miR-486-3p downregulates the main pacemaking channel HCN4 in the SN. In addition, we recently demonstrated that immune cells are significantly more abundant in the SN compared to the right atrium. The aim of this study was to validate the previously predicted interactions between miRNAs and mRNAs of key Ca<sup>2+</sup> ion channels (involved in peacemaking) and mRNA of TPSAB1—(a mast cells marker) using luciferase assay. We now show that miR-486 significantly downregulates Ca<sub>v</sub>1.3, Ca<sub>v</sub>3.1, and TPSAB1-mediated luciferase activity, while miR-938 significantly downregulates only TPSAB1-mediated luciferase activity. This makes miR-486-3p a potential therapeutic target in the treatment of SN dysfunctions. |
| first_indexed | 2024-03-10T04:57:41Z |
| format | Article |
| id | doaj.art-d995cc1fe45240a596d5914b13b0913a |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T04:57:41Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-d995cc1fe45240a596d5914b13b0913a2023-11-23T02:07:01ZengMDPI AGApplied Sciences2076-34172021-12-0111231136610.3390/app112311366MiR-486-3p and MiR-938—Important Inhibitors of Pacemaking Ion Channels and/or Markers of Immune CellsAbimbola J Aminu0Maria Petkova1Weixuan Chen2Zeyuan Yin3Vlad S Kuzmin4Andrew J Atkinson5Halina Dobrzynski6Cardiovascular Sciences, School of Medicine, University of Manchester, Manchester M13 9PL, UKCardiovascular Sciences, School of Medicine, University of Manchester, Manchester M13 9PL, UKCardiovascular Sciences, School of Medicine, University of Manchester, Manchester M13 9PL, UKCardiovascular Sciences, School of Medicine, University of Manchester, Manchester M13 9PL, UKBiological Faculty, Department of Human and Animal Physiology, Leninskie Gory 1, Building 2, Lomonosov Moscow State University, 119991 Moscow, RussiaCardiovascular Sciences, School of Medicine, University of Manchester, Manchester M13 9PL, UKCardiovascular Sciences, School of Medicine, University of Manchester, Manchester M13 9PL, UKThe sinus node (SN) is the heart’s primary pacemaker and has a unique expression of pacemaking ion channels and immune cell markers. The role of microribonucleic acids (miRNAs) in control of ion channels and immune function of the sinus node is not well understood. We have recently shown that hsa-miR-486-3p downregulates the main pacemaking channel HCN4 in the SN. In addition, we recently demonstrated that immune cells are significantly more abundant in the SN compared to the right atrium. The aim of this study was to validate the previously predicted interactions between miRNAs and mRNAs of key Ca<sup>2+</sup> ion channels (involved in peacemaking) and mRNA of TPSAB1—(a mast cells marker) using luciferase assay. We now show that miR-486 significantly downregulates Ca<sub>v</sub>1.3, Ca<sub>v</sub>3.1, and TPSAB1-mediated luciferase activity, while miR-938 significantly downregulates only TPSAB1-mediated luciferase activity. This makes miR-486-3p a potential therapeutic target in the treatment of SN dysfunctions.https://www.mdpi.com/2076-3417/11/23/11366miR-486-3pmiR-938Ca<sub>v</sub>1.3Ca<sub>v</sub>3.1TPSAB1sinus node |
| spellingShingle | Abimbola J Aminu Maria Petkova Weixuan Chen Zeyuan Yin Vlad S Kuzmin Andrew J Atkinson Halina Dobrzynski MiR-486-3p and MiR-938—Important Inhibitors of Pacemaking Ion Channels and/or Markers of Immune Cells Applied Sciences miR-486-3p miR-938 Ca<sub>v</sub>1.3 Ca<sub>v</sub>3.1 TPSAB1 sinus node |
| title | MiR-486-3p and MiR-938—Important Inhibitors of Pacemaking Ion Channels and/or Markers of Immune Cells |
| title_full | MiR-486-3p and MiR-938—Important Inhibitors of Pacemaking Ion Channels and/or Markers of Immune Cells |
| title_fullStr | MiR-486-3p and MiR-938—Important Inhibitors of Pacemaking Ion Channels and/or Markers of Immune Cells |
| title_full_unstemmed | MiR-486-3p and MiR-938—Important Inhibitors of Pacemaking Ion Channels and/or Markers of Immune Cells |
| title_short | MiR-486-3p and MiR-938—Important Inhibitors of Pacemaking Ion Channels and/or Markers of Immune Cells |
| title_sort | mir 486 3p and mir 938 important inhibitors of pacemaking ion channels and or markers of immune cells |
| topic | miR-486-3p miR-938 Ca<sub>v</sub>1.3 Ca<sub>v</sub>3.1 TPSAB1 sinus node |
| url | https://www.mdpi.com/2076-3417/11/23/11366 |
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