The Composition and Diversity of Intestinal Microbes at Different Growth Stages of Giant Freshwater Prawns (<i>Macrobrachium rosenbergii</i>)
Intestinal bacterial communities play an important role in the growth and health of aquatic animal hosts and have drawn increasing attention. However, the role of the intestinal microbiota in the growth of freshwater prawns remains unclear. Here, the intestinal microbiota of freshwater prawns (<i...
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MDPI AG
2023-09-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/8/9/473 |
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| author | Hongxiang Xu Jindong Ren Xiaojun Xu Bao Lou Demin Zhang |
| author_facet | Hongxiang Xu Jindong Ren Xiaojun Xu Bao Lou Demin Zhang |
| author_sort | Hongxiang Xu |
| collection | DOAJ |
| description | Intestinal bacterial communities play an important role in the growth and health of aquatic animal hosts and have drawn increasing attention. However, the role of the intestinal microbiota in the growth of freshwater prawns remains unclear. Here, the intestinal microbiota of freshwater prawns (<i>Macrobrachium rosenbergii</i>) at different life stages (one, two, and three months old) were investigated using 16S rRNA sequencing. The results showed that community richness and diversity increased with growth, which might be one of the reasons that the prawns maintained a fast growth rate before sexual maturation. Three core phyla were identified in the one-month-old group, namely, <i>Firmicutes</i> (79.24%), <i>Proteobacteria</i> (17.09%) and <i>Actinobacteriota</i> (2.01%). Five core phyla were identified in the two-month-old group, including <i>Firmicutes</i> (47.84%), <i>Proteobacteria</i> (44.22%), <i>Actinobacteriota</i> (1.83%), <i>Acidobacteriota</i> (1.66%) and <i>Bacteroidota</i> (1.24%), and the core phyla in the three-month-old group were similar, except for <i>Acidobacterota,</i> which was not identified. A total of 12 core genera were identified in all samples, and significant differences were observed in the relative abundance of gut microbiota between the three groups (<i>p</i> < 0.05). <i>Exiguobacterium</i>, <i>Lactococcus</i> and <i>Shewanella</i> were the three most significantly differentially abundant genera between stages. In addition, <i>Candidatus Hepatoplasma</i> was detected only in the two- and three-month-old prawn groups. This study provides information on the differences in the intestinal microbiota in different developmental stages, which contribute to adaptation to salinity in the early developmental stage and digestive ability to meet the growth needs of <i>Macrobrachium rosenbergii</i>. |
| first_indexed | 2024-03-10T22:46:42Z |
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| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-10T22:46:42Z |
| publishDate | 2023-09-01 |
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| spelling | doaj.art-d99bcde2e4714a23b979df9307194ab22023-11-19T10:41:11ZengMDPI AGFishes2410-38882023-09-018947310.3390/fishes8090473The Composition and Diversity of Intestinal Microbes at Different Growth Stages of Giant Freshwater Prawns (<i>Macrobrachium rosenbergii</i>)Hongxiang Xu0Jindong Ren1Xiaojun Xu2Bao Lou3Demin Zhang4School of Marine Science, Ningbo University, Ningbo 315211, ChinaInstitute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaSchool of Marine Science, Ningbo University, Ningbo 315211, ChinaIntestinal bacterial communities play an important role in the growth and health of aquatic animal hosts and have drawn increasing attention. However, the role of the intestinal microbiota in the growth of freshwater prawns remains unclear. Here, the intestinal microbiota of freshwater prawns (<i>Macrobrachium rosenbergii</i>) at different life stages (one, two, and three months old) were investigated using 16S rRNA sequencing. The results showed that community richness and diversity increased with growth, which might be one of the reasons that the prawns maintained a fast growth rate before sexual maturation. Three core phyla were identified in the one-month-old group, namely, <i>Firmicutes</i> (79.24%), <i>Proteobacteria</i> (17.09%) and <i>Actinobacteriota</i> (2.01%). Five core phyla were identified in the two-month-old group, including <i>Firmicutes</i> (47.84%), <i>Proteobacteria</i> (44.22%), <i>Actinobacteriota</i> (1.83%), <i>Acidobacteriota</i> (1.66%) and <i>Bacteroidota</i> (1.24%), and the core phyla in the three-month-old group were similar, except for <i>Acidobacterota,</i> which was not identified. A total of 12 core genera were identified in all samples, and significant differences were observed in the relative abundance of gut microbiota between the three groups (<i>p</i> < 0.05). <i>Exiguobacterium</i>, <i>Lactococcus</i> and <i>Shewanella</i> were the three most significantly differentially abundant genera between stages. In addition, <i>Candidatus Hepatoplasma</i> was detected only in the two- and three-month-old prawn groups. This study provides information on the differences in the intestinal microbiota in different developmental stages, which contribute to adaptation to salinity in the early developmental stage and digestive ability to meet the growth needs of <i>Macrobrachium rosenbergii</i>.https://www.mdpi.com/2410-3888/8/9/473intestinal microbiotacomposition diversity<i>Macrobrachium rosenbergii</i> |
| spellingShingle | Hongxiang Xu Jindong Ren Xiaojun Xu Bao Lou Demin Zhang The Composition and Diversity of Intestinal Microbes at Different Growth Stages of Giant Freshwater Prawns (<i>Macrobrachium rosenbergii</i>) Fishes intestinal microbiota composition diversity <i>Macrobrachium rosenbergii</i> |
| title | The Composition and Diversity of Intestinal Microbes at Different Growth Stages of Giant Freshwater Prawns (<i>Macrobrachium rosenbergii</i>) |
| title_full | The Composition and Diversity of Intestinal Microbes at Different Growth Stages of Giant Freshwater Prawns (<i>Macrobrachium rosenbergii</i>) |
| title_fullStr | The Composition and Diversity of Intestinal Microbes at Different Growth Stages of Giant Freshwater Prawns (<i>Macrobrachium rosenbergii</i>) |
| title_full_unstemmed | The Composition and Diversity of Intestinal Microbes at Different Growth Stages of Giant Freshwater Prawns (<i>Macrobrachium rosenbergii</i>) |
| title_short | The Composition and Diversity of Intestinal Microbes at Different Growth Stages of Giant Freshwater Prawns (<i>Macrobrachium rosenbergii</i>) |
| title_sort | composition and diversity of intestinal microbes at different growth stages of giant freshwater prawns i macrobrachium rosenbergii i |
| topic | intestinal microbiota composition diversity <i>Macrobrachium rosenbergii</i> |
| url | https://www.mdpi.com/2410-3888/8/9/473 |
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