New Sequence Type ST3449 in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Isolates from a Cystic Fibrosis Patient
<i>Pseudomonas aeruginosa</i> is one of the most critical bacterial pathogens associated with chronic infections in cystic fibrosis patients. Here we show the phenotypic and genotypic characterization of five consecutive multidrug-resistant isolates of <i>P. aeruginosa</i> co...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/5/491 |
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| author | Catalina Díaz-Ríos Marta Hernández David Abad Laura Álvarez-Montes Athanasia Varsaki David Iturbe Jorge Calvo Alain A. Ocampo-Sosa |
| author_facet | Catalina Díaz-Ríos Marta Hernández David Abad Laura Álvarez-Montes Athanasia Varsaki David Iturbe Jorge Calvo Alain A. Ocampo-Sosa |
| author_sort | Catalina Díaz-Ríos |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> is one of the most critical bacterial pathogens associated with chronic infections in cystic fibrosis patients. Here we show the phenotypic and genotypic characterization of five consecutive multidrug-resistant isolates of <i>P. aeruginosa</i> collected during a month from a CF patient with end-stage lung disease and fatal outcome. The isolates exhibited distinct colony morphologies and pigmentation and differences in their capacity to produce biofilm and virulence potential evaluated in larvae of <i>Galleria mellonella</i>. Whole genome-sequencing showed that isolates belonged to a novel sequence type ST3449 and serotype O6. Analysis of their resistome demonstrated the presence of genes <i>bla</i><sub>OXA-396</sub>, <i>bla</i><sub>PAO</sub>, <i>aph(3’)-IIb</i>, <i>catB</i>, <i>crpP</i> and <i>fosA</i> and new mutations in chromosomal genes conferring resistance to different antipseudomonal antibiotics. Genes <i>exoS</i>, <i>exoT</i>, <i>exoY</i>, <i>toxA</i>, <i>lasI</i>, <i>rhlI</i> and <i>tse1</i> were among the 220 virulence genes detected. The different phenotypic and genotypic features found reveal the adaptation of clone ST3449 to the CF lung environment by a number of mutations affecting genes related with biofilm formation, quorum sensing and antimicrobial resistance. Most of these mutations are commonly found in CF isolates, which may give us important clues for future development of new drug targets to combat <i>P. aeruginosa</i> chronic infections. |
| first_indexed | 2024-03-10T12:02:18Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T12:02:18Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-d99e7dea2e21419aa1300e64d370fb0b2023-11-21T16:54:16ZengMDPI AGAntibiotics2079-63822021-04-0110549110.3390/antibiotics10050491New Sequence Type ST3449 in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Isolates from a Cystic Fibrosis PatientCatalina Díaz-Ríos0Marta Hernández1David Abad2Laura Álvarez-Montes3Athanasia Varsaki4David Iturbe5Jorge Calvo6Alain A. Ocampo-Sosa7Instituto de Investigación Sanitaria Marqués de Valdecilla (IDIVAL), 39011 Santander, SpainInstituto Tecnológico Agrario de Castilla y León (ITACyL), 47071 Valladolid, SpainInstituto Tecnológico Agrario de Castilla y León (ITACyL), 47071 Valladolid, SpainInstituto de Investigación Sanitaria Marqués de Valdecilla (IDIVAL), 39011 Santander, SpainCentro de Investigación y Formación Agraria (CIFA), 39600 Muriedas, SpainServicio de Neumología, Hospital Universitario Marqués de Valdecilla, 39008 Santander, SpainServicio de Microbiología, Hospital Universitario Marqués de Valdecilla, 39008 Santander, SpainInstituto de Investigación Sanitaria Marqués de Valdecilla (IDIVAL), 39011 Santander, Spain<i>Pseudomonas aeruginosa</i> is one of the most critical bacterial pathogens associated with chronic infections in cystic fibrosis patients. Here we show the phenotypic and genotypic characterization of five consecutive multidrug-resistant isolates of <i>P. aeruginosa</i> collected during a month from a CF patient with end-stage lung disease and fatal outcome. The isolates exhibited distinct colony morphologies and pigmentation and differences in their capacity to produce biofilm and virulence potential evaluated in larvae of <i>Galleria mellonella</i>. Whole genome-sequencing showed that isolates belonged to a novel sequence type ST3449 and serotype O6. Analysis of their resistome demonstrated the presence of genes <i>bla</i><sub>OXA-396</sub>, <i>bla</i><sub>PAO</sub>, <i>aph(3’)-IIb</i>, <i>catB</i>, <i>crpP</i> and <i>fosA</i> and new mutations in chromosomal genes conferring resistance to different antipseudomonal antibiotics. Genes <i>exoS</i>, <i>exoT</i>, <i>exoY</i>, <i>toxA</i>, <i>lasI</i>, <i>rhlI</i> and <i>tse1</i> were among the 220 virulence genes detected. The different phenotypic and genotypic features found reveal the adaptation of clone ST3449 to the CF lung environment by a number of mutations affecting genes related with biofilm formation, quorum sensing and antimicrobial resistance. Most of these mutations are commonly found in CF isolates, which may give us important clues for future development of new drug targets to combat <i>P. aeruginosa</i> chronic infections.https://www.mdpi.com/2079-6382/10/5/491<i>Pseudomonas aeruginosa</i>sequence type ST3449multi-drug resistancecystic fibrosisbiofilmvirulence |
| spellingShingle | Catalina Díaz-Ríos Marta Hernández David Abad Laura Álvarez-Montes Athanasia Varsaki David Iturbe Jorge Calvo Alain A. Ocampo-Sosa New Sequence Type ST3449 in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Isolates from a Cystic Fibrosis Patient Antibiotics <i>Pseudomonas aeruginosa</i> sequence type ST3449 multi-drug resistance cystic fibrosis biofilm virulence |
| title | New Sequence Type ST3449 in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Isolates from a Cystic Fibrosis Patient |
| title_full | New Sequence Type ST3449 in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Isolates from a Cystic Fibrosis Patient |
| title_fullStr | New Sequence Type ST3449 in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Isolates from a Cystic Fibrosis Patient |
| title_full_unstemmed | New Sequence Type ST3449 in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Isolates from a Cystic Fibrosis Patient |
| title_short | New Sequence Type ST3449 in Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Isolates from a Cystic Fibrosis Patient |
| title_sort | new sequence type st3449 in multidrug resistant i pseudomonas aeruginosa i isolates from a cystic fibrosis patient |
| topic | <i>Pseudomonas aeruginosa</i> sequence type ST3449 multi-drug resistance cystic fibrosis biofilm virulence |
| url | https://www.mdpi.com/2079-6382/10/5/491 |
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