Detailed mitochondrial phenotyping by high resolution metabolomics.
Mitochondrial phenotype is complex and difficult to define at the level of individual cell types. Newer metabolic profiling methods provide information on dozens of metabolic pathways from a relatively small sample. This pilot study used "top-down" metabolic profiling to determine the spec...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3295783?pdf=render |
| _version_ | 1818254331338555392 |
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| author | James R Roede Youngja Park Shuzhao Li Frederick H Strobel Dean P Jones |
| author_facet | James R Roede Youngja Park Shuzhao Li Frederick H Strobel Dean P Jones |
| author_sort | James R Roede |
| collection | DOAJ |
| description | Mitochondrial phenotype is complex and difficult to define at the level of individual cell types. Newer metabolic profiling methods provide information on dozens of metabolic pathways from a relatively small sample. This pilot study used "top-down" metabolic profiling to determine the spectrum of metabolites present in liver mitochondria. High resolution mass spectral analyses and multivariate statistical tests provided global metabolic information about mitochondria and showed that liver mitochondria possess a significant phenotype based on gender and genotype. The data also show that mitochondria contain a large number of unidentified chemicals. |
| first_indexed | 2024-12-12T16:54:16Z |
| format | Article |
| id | doaj.art-d9a09667df44436392445c04a77c841f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T16:54:16Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d9a09667df44436392445c04a77c841f2022-12-22T00:18:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3302010.1371/journal.pone.0033020Detailed mitochondrial phenotyping by high resolution metabolomics.James R RoedeYoungja ParkShuzhao LiFrederick H StrobelDean P JonesMitochondrial phenotype is complex and difficult to define at the level of individual cell types. Newer metabolic profiling methods provide information on dozens of metabolic pathways from a relatively small sample. This pilot study used "top-down" metabolic profiling to determine the spectrum of metabolites present in liver mitochondria. High resolution mass spectral analyses and multivariate statistical tests provided global metabolic information about mitochondria and showed that liver mitochondria possess a significant phenotype based on gender and genotype. The data also show that mitochondria contain a large number of unidentified chemicals.http://europepmc.org/articles/PMC3295783?pdf=render |
| spellingShingle | James R Roede Youngja Park Shuzhao Li Frederick H Strobel Dean P Jones Detailed mitochondrial phenotyping by high resolution metabolomics. PLoS ONE |
| title | Detailed mitochondrial phenotyping by high resolution metabolomics. |
| title_full | Detailed mitochondrial phenotyping by high resolution metabolomics. |
| title_fullStr | Detailed mitochondrial phenotyping by high resolution metabolomics. |
| title_full_unstemmed | Detailed mitochondrial phenotyping by high resolution metabolomics. |
| title_short | Detailed mitochondrial phenotyping by high resolution metabolomics. |
| title_sort | detailed mitochondrial phenotyping by high resolution metabolomics |
| url | http://europepmc.org/articles/PMC3295783?pdf=render |
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