Phylogenetic position and replication kinetics of Heliothis virescens ascovirus 3h (HvAV-3h) isolated from Spodoptera exigua.
Insect-specific ascoviruses with a circular genome are distributed in the USA, France, Australia and Indonesia. Here, we report the first ascovirus isolation from Spodoptera exigua in Hunan, China. DNA-DNA hybridization to published ascoviruses demonstrated that the new China ascovirus isolate is a...
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Public Library of Science (PLoS)
2012-01-01
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22792245/?tool=EBI |
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| author | Guo-Hua Huang Tyler A Garretson Xin-Hua Cheng Maria S Holztrager Shun-Ji Li Xing Wang Xiao-Wen Cheng |
| author_facet | Guo-Hua Huang Tyler A Garretson Xin-Hua Cheng Maria S Holztrager Shun-Ji Li Xing Wang Xiao-Wen Cheng |
| author_sort | Guo-Hua Huang |
| collection | DOAJ |
| description | Insect-specific ascoviruses with a circular genome are distributed in the USA, France, Australia and Indonesia. Here, we report the first ascovirus isolation from Spodoptera exigua in Hunan, China. DNA-DNA hybridization to published ascoviruses demonstrated that the new China ascovirus isolate is a variant of Heliothis virescens ascovirus 3a (HvAV-3a), thus named HvAV-3h. We investigated the phylogenetic position, cell infection, vesicle production and viral DNA replication kinetics of HvAV-3h, as well as its host-ranges. The major capsid protein (MCP) gene and the delta DNA polymerase (DNA po1) gene of HvAV-3h were sequenced and compared with the available ascovirus isolates for phylogenetic analysis. This shows a close relationship with HvAV-3g, originally isolated from Indonesia, HvAV-3e from Australia and HvAV-3c from United States. HvAV-3h infection induced vesicle production in the SeE1 cells derived from S. exigua and Sf9 cells derived from S. frugiperda, resulting in more vesicles generated in Sf9 than SeE1. Viral DNA replication kinetics of HvAV-3h also demonstrated a difference between the two cell lines tested. HvAV-3h could readily infect three important insect pests Helicoverpa armigera (Hübner), Spodoptera exigua (Hübner) and Spodoptera litura (Fabricius) from two genera in different subfamilies with high mortalities. |
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| language | English |
| last_indexed | 2024-12-17T20:59:47Z |
| publishDate | 2012-01-01 |
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| spelling | doaj.art-d9a340b5616341aa847a52f847dc31032022-12-21T21:32:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4022510.1371/journal.pone.0040225Phylogenetic position and replication kinetics of Heliothis virescens ascovirus 3h (HvAV-3h) isolated from Spodoptera exigua.Guo-Hua HuangTyler A GarretsonXin-Hua ChengMaria S HolztragerShun-Ji LiXing WangXiao-Wen ChengInsect-specific ascoviruses with a circular genome are distributed in the USA, France, Australia and Indonesia. Here, we report the first ascovirus isolation from Spodoptera exigua in Hunan, China. DNA-DNA hybridization to published ascoviruses demonstrated that the new China ascovirus isolate is a variant of Heliothis virescens ascovirus 3a (HvAV-3a), thus named HvAV-3h. We investigated the phylogenetic position, cell infection, vesicle production and viral DNA replication kinetics of HvAV-3h, as well as its host-ranges. The major capsid protein (MCP) gene and the delta DNA polymerase (DNA po1) gene of HvAV-3h were sequenced and compared with the available ascovirus isolates for phylogenetic analysis. This shows a close relationship with HvAV-3g, originally isolated from Indonesia, HvAV-3e from Australia and HvAV-3c from United States. HvAV-3h infection induced vesicle production in the SeE1 cells derived from S. exigua and Sf9 cells derived from S. frugiperda, resulting in more vesicles generated in Sf9 than SeE1. Viral DNA replication kinetics of HvAV-3h also demonstrated a difference between the two cell lines tested. HvAV-3h could readily infect three important insect pests Helicoverpa armigera (Hübner), Spodoptera exigua (Hübner) and Spodoptera litura (Fabricius) from two genera in different subfamilies with high mortalities.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22792245/?tool=EBI |
| spellingShingle | Guo-Hua Huang Tyler A Garretson Xin-Hua Cheng Maria S Holztrager Shun-Ji Li Xing Wang Xiao-Wen Cheng Phylogenetic position and replication kinetics of Heliothis virescens ascovirus 3h (HvAV-3h) isolated from Spodoptera exigua. PLoS ONE |
| title | Phylogenetic position and replication kinetics of Heliothis virescens ascovirus 3h (HvAV-3h) isolated from Spodoptera exigua. |
| title_full | Phylogenetic position and replication kinetics of Heliothis virescens ascovirus 3h (HvAV-3h) isolated from Spodoptera exigua. |
| title_fullStr | Phylogenetic position and replication kinetics of Heliothis virescens ascovirus 3h (HvAV-3h) isolated from Spodoptera exigua. |
| title_full_unstemmed | Phylogenetic position and replication kinetics of Heliothis virescens ascovirus 3h (HvAV-3h) isolated from Spodoptera exigua. |
| title_short | Phylogenetic position and replication kinetics of Heliothis virescens ascovirus 3h (HvAV-3h) isolated from Spodoptera exigua. |
| title_sort | phylogenetic position and replication kinetics of heliothis virescens ascovirus 3h hvav 3h isolated from spodoptera exigua |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22792245/?tool=EBI |
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