Interaction Insight of Pullulan-Mediated Gamma-Irradiated Silver Nanoparticle Synthesis and Its Antibacterial Activity
The production of pure silver nanoparticles (Ag-NPs) with unique properties remains a challenge even today. In the present study, the synthesis of silver nanoparticles (Ag-NPs) from natural pullulan (PL) was carried out using a radiation-induced method. It is known that pullulan is regarded as a mic...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/20/3578 |
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| author | Mohd Shahrul Nizam Salleh Roshafima Rasit Ali Kamyar Shameli Mohd Yusof Hamzah Rafiziana Md Kasmani Mohamed Mahmoud Nasef |
| author_facet | Mohd Shahrul Nizam Salleh Roshafima Rasit Ali Kamyar Shameli Mohd Yusof Hamzah Rafiziana Md Kasmani Mohamed Mahmoud Nasef |
| author_sort | Mohd Shahrul Nizam Salleh |
| collection | DOAJ |
| description | The production of pure silver nanoparticles (Ag-NPs) with unique properties remains a challenge even today. In the present study, the synthesis of silver nanoparticles (Ag-NPs) from natural pullulan (PL) was carried out using a radiation-induced method. It is known that pullulan is regarded as a microbial polysaccharide, which renders it suitable to act as a reducing and stabilizing agent during the production of Ag-NPs. Pullulan-assisted synthesis under gamma irradiation was successfully developed to obtain Ag-NPs, which was characterized by UV-Vis, XRD, TEM, and Zeta potential analysis. Pullulan was used as a stabilizer and template for the growth of silver nanoparticles, while gamma radiation was modified to be selective to reduce silver ions. The formation of Ag-NPs was confirmed using UV–Vis spectra by showing a surface plasmon resonance (SPR) band in the region of 410–420 nm. As observed by TEM images, it can be said that by increasing the radiation dose, the particle size decreases, resulting in a mean diameter of Ag-NPs ranging from 40.97 to 3.98 nm. The XRD analysis confirmed that silver metal structures with a face-centered cubic (FCC) crystal were present, while TEM images showed a spherical shape with smooth edges. XRD also demonstrated that increasing the dose of gamma radiation increases the crystallinity at a high purity of Ag-NPs. As examined by zeta potential, the synthesized Ag-NP/PL was negatively charged with high stability. Ag-NP/PL was then analysed for antimicrobial activity against <i>Staphylococcus aureus</i>, and it was found that it had high antibacterial activity. It is found that the adoption of radiation doses results in a stable and green reduction process for silver nanoparticles. |
| first_indexed | 2024-03-10T06:14:44Z |
| format | Article |
| id | doaj.art-d9a55b936318447d9dcf0e09b62a08e6 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T06:14:44Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-d9a55b936318447d9dcf0e09b62a08e62023-11-22T19:48:01ZengMDPI AGPolymers2073-43602021-10-011320357810.3390/polym13203578Interaction Insight of Pullulan-Mediated Gamma-Irradiated Silver Nanoparticle Synthesis and Its Antibacterial ActivityMohd Shahrul Nizam Salleh0Roshafima Rasit Ali1Kamyar Shameli2Mohd Yusof Hamzah3Rafiziana Md Kasmani4Mohamed Mahmoud Nasef5School of Chemical Engineering, College of Engineering, Universiti Teknologi MARA Cawangan Terengganu, Bukit Besi Campus, Dungun 23200, Terengganu, MalaysiaChemical Process Engineering Department, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Ahmad Petra, Kuala Lumpur 54100, Johor, MalaysiaChemical Process Engineering Department, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Ahmad Petra, Kuala Lumpur 54100, Johor, MalaysiaRadiation Processing Technology Division, Malaysia Nuclear Agency, Kajang 43000, Selangor, MalaysiaDepartment of Renewable Energy Engineering, Faculty of Chemical & Energy Engineering, Universiti Teknologi Malaysia, Johor Bahru 81310, Johor, MalaysiaChemical Process Engineering Department, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Ahmad Petra, Kuala Lumpur 54100, Johor, MalaysiaThe production of pure silver nanoparticles (Ag-NPs) with unique properties remains a challenge even today. In the present study, the synthesis of silver nanoparticles (Ag-NPs) from natural pullulan (PL) was carried out using a radiation-induced method. It is known that pullulan is regarded as a microbial polysaccharide, which renders it suitable to act as a reducing and stabilizing agent during the production of Ag-NPs. Pullulan-assisted synthesis under gamma irradiation was successfully developed to obtain Ag-NPs, which was characterized by UV-Vis, XRD, TEM, and Zeta potential analysis. Pullulan was used as a stabilizer and template for the growth of silver nanoparticles, while gamma radiation was modified to be selective to reduce silver ions. The formation of Ag-NPs was confirmed using UV–Vis spectra by showing a surface plasmon resonance (SPR) band in the region of 410–420 nm. As observed by TEM images, it can be said that by increasing the radiation dose, the particle size decreases, resulting in a mean diameter of Ag-NPs ranging from 40.97 to 3.98 nm. The XRD analysis confirmed that silver metal structures with a face-centered cubic (FCC) crystal were present, while TEM images showed a spherical shape with smooth edges. XRD also demonstrated that increasing the dose of gamma radiation increases the crystallinity at a high purity of Ag-NPs. As examined by zeta potential, the synthesized Ag-NP/PL was negatively charged with high stability. Ag-NP/PL was then analysed for antimicrobial activity against <i>Staphylococcus aureus</i>, and it was found that it had high antibacterial activity. It is found that the adoption of radiation doses results in a stable and green reduction process for silver nanoparticles.https://www.mdpi.com/2073-4360/13/20/3578silver nanoparticlespullulangamma irradiationgreen synthesisantibacterial |
| spellingShingle | Mohd Shahrul Nizam Salleh Roshafima Rasit Ali Kamyar Shameli Mohd Yusof Hamzah Rafiziana Md Kasmani Mohamed Mahmoud Nasef Interaction Insight of Pullulan-Mediated Gamma-Irradiated Silver Nanoparticle Synthesis and Its Antibacterial Activity Polymers silver nanoparticles pullulan gamma irradiation green synthesis antibacterial |
| title | Interaction Insight of Pullulan-Mediated Gamma-Irradiated Silver Nanoparticle Synthesis and Its Antibacterial Activity |
| title_full | Interaction Insight of Pullulan-Mediated Gamma-Irradiated Silver Nanoparticle Synthesis and Its Antibacterial Activity |
| title_fullStr | Interaction Insight of Pullulan-Mediated Gamma-Irradiated Silver Nanoparticle Synthesis and Its Antibacterial Activity |
| title_full_unstemmed | Interaction Insight of Pullulan-Mediated Gamma-Irradiated Silver Nanoparticle Synthesis and Its Antibacterial Activity |
| title_short | Interaction Insight of Pullulan-Mediated Gamma-Irradiated Silver Nanoparticle Synthesis and Its Antibacterial Activity |
| title_sort | interaction insight of pullulan mediated gamma irradiated silver nanoparticle synthesis and its antibacterial activity |
| topic | silver nanoparticles pullulan gamma irradiation green synthesis antibacterial |
| url | https://www.mdpi.com/2073-4360/13/20/3578 |
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