High-Pressure Inactivation of <i>Bacillus cereus</i> in Human Breast Milk
Although Holder pasteurization is the recommended method for processing breast milk, it does affect some of its nutritional and biological properties and is ineffective at inactivating spores. The aim of this study was to find and validate an alternative methodology for processing breast milk to inc...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/23/4245 |
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| author | Miroslava Jandová Michaela Fišerová Pavla Paterová Lucie Cacková Pavel Měřička Jan Malý Marian Kacerovský Eliška Kovaříková Jan Strohalm Kateřina Demnerová Jana Kadavá Hana Sýkorová Radomír Hyšpler Dana Čížková Aleš Bezrouk Milan Houška |
| author_facet | Miroslava Jandová Michaela Fišerová Pavla Paterová Lucie Cacková Pavel Měřička Jan Malý Marian Kacerovský Eliška Kovaříková Jan Strohalm Kateřina Demnerová Jana Kadavá Hana Sýkorová Radomír Hyšpler Dana Čížková Aleš Bezrouk Milan Houška |
| author_sort | Miroslava Jandová |
| collection | DOAJ |
| description | Although Holder pasteurization is the recommended method for processing breast milk, it does affect some of its nutritional and biological properties and is ineffective at inactivating spores. The aim of this study was to find and validate an alternative methodology for processing breast milk to increase its availability for newborn babies and reduce the financial loss associated with discarding milk that has become microbiologically positive. We prepared two series of breast milk samples inoculated with the <i>Bacillus cereus</i> (<i>B. cereus</i>) strain to verify the effectiveness of two high-pressure treatments: (1) 350 MPa/5 min/38 °C in four cycles and (2) cumulative pressure of 350 MPa/20 min/38 °C. We found that the use of pressure in cycles was statistically more effective than cumulative pressure. It reduced the number of spores by three to four orders of magnitude. We verified that the method was reproducible. The routine use of this method could lead to an increased availability of milk for newborn babies, and at the same time, reduce the amount of wasted milk. In addition, high-pressure treatment preserves the nutritional quality of milk. |
| first_indexed | 2024-03-09T01:52:18Z |
| format | Article |
| id | doaj.art-d9a75ff73a034789b24318ee85f7aafa |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T01:52:18Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-d9a75ff73a034789b24318ee85f7aafa2023-12-08T15:15:27ZengMDPI AGFoods2304-81582023-11-011223424510.3390/foods12234245High-Pressure Inactivation of <i>Bacillus cereus</i> in Human Breast MilkMiroslava Jandová0Michaela Fišerová1Pavla Paterová2Lucie Cacková3Pavel Měřička4Jan Malý5Marian Kacerovský6Eliška Kovaříková7Jan Strohalm8Kateřina Demnerová9Jana Kadavá10Hana Sýkorová11Radomír Hyšpler12Dana Čížková13Aleš Bezrouk14Milan Houška15Tissue Bank, University Hospital Hradec Králové, 500 05 Hradec Králové, Czech RepublicTissue Bank, University Hospital Hradec Králové, 500 05 Hradec Králové, Czech RepublicDepartment of Clinical Microbiology, University Hospital and Faculty of Medicine in Hradec Králové, Charles University, 500 05 Hradec Králové, Czech RepublicDepartment of Clinical Microbiology, University Hospital and Faculty of Medicine in Hradec Králové, Charles University, 500 05 Hradec Králové, Czech RepublicTissue Bank, University Hospital Hradec Králové, 500 05 Hradec Králové, Czech RepublicDepartment of Pediatrics, University Hospital Hradec Králové, 500 05 Hradec Králové, Czech RepublicBiomedical Research Center, University Hospital Hradec Králové, 500 05 Hradec Králové, Czech RepublicFood Research Institute Prague, 102 00 Prague, Czech RepublicFood Research Institute Prague, 102 00 Prague, Czech RepublicDepartment of Biochemistry and Microbiology, University of Chemistry and Technology Prague, 166 28 Prague, Czech RepublicDepartment of Biochemistry and Microbiology, University of Chemistry and Technology Prague, 166 28 Prague, Czech RepublicDepartment of Biochemistry and Microbiology, University of Chemistry and Technology Prague, 166 28 Prague, Czech RepublicDepartment of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, 500 05 Hradec Králové, Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine in Hradec Králové, Charles University, 500 03 Hradec Králové, Czech RepublicDepartment of Medical Biophysics, Faculty of Medicine in Hradec Králové, Charles University, 500 03 Hradec Králové, Czech RepublicFood Research Institute Prague, 102 00 Prague, Czech RepublicAlthough Holder pasteurization is the recommended method for processing breast milk, it does affect some of its nutritional and biological properties and is ineffective at inactivating spores. The aim of this study was to find and validate an alternative methodology for processing breast milk to increase its availability for newborn babies and reduce the financial loss associated with discarding milk that has become microbiologically positive. We prepared two series of breast milk samples inoculated with the <i>Bacillus cereus</i> (<i>B. cereus</i>) strain to verify the effectiveness of two high-pressure treatments: (1) 350 MPa/5 min/38 °C in four cycles and (2) cumulative pressure of 350 MPa/20 min/38 °C. We found that the use of pressure in cycles was statistically more effective than cumulative pressure. It reduced the number of spores by three to four orders of magnitude. We verified that the method was reproducible. The routine use of this method could lead to an increased availability of milk for newborn babies, and at the same time, reduce the amount of wasted milk. In addition, high-pressure treatment preserves the nutritional quality of milk.https://www.mdpi.com/2304-8158/12/23/4245pressurization<i>Bacillus cereus</i>human breast milkinoculation |
| spellingShingle | Miroslava Jandová Michaela Fišerová Pavla Paterová Lucie Cacková Pavel Měřička Jan Malý Marian Kacerovský Eliška Kovaříková Jan Strohalm Kateřina Demnerová Jana Kadavá Hana Sýkorová Radomír Hyšpler Dana Čížková Aleš Bezrouk Milan Houška High-Pressure Inactivation of <i>Bacillus cereus</i> in Human Breast Milk Foods pressurization <i>Bacillus cereus</i> human breast milk inoculation |
| title | High-Pressure Inactivation of <i>Bacillus cereus</i> in Human Breast Milk |
| title_full | High-Pressure Inactivation of <i>Bacillus cereus</i> in Human Breast Milk |
| title_fullStr | High-Pressure Inactivation of <i>Bacillus cereus</i> in Human Breast Milk |
| title_full_unstemmed | High-Pressure Inactivation of <i>Bacillus cereus</i> in Human Breast Milk |
| title_short | High-Pressure Inactivation of <i>Bacillus cereus</i> in Human Breast Milk |
| title_sort | high pressure inactivation of i bacillus cereus i in human breast milk |
| topic | pressurization <i>Bacillus cereus</i> human breast milk inoculation |
| url | https://www.mdpi.com/2304-8158/12/23/4245 |
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