Evaluation of phage—antibiotic combinations in the treatment of extended-spectrum β-lactamase-producing Salmonella enteritidis strain PT1
Foodborne infections caused by Salmonella spp. are among the most common foodborne diseases in the world. We isolated a lytic phage against extended-spectrum beta-lactam producing S. Enteritidis strain PT1 derived from chicken carcass. Results from electronmicrography indicated that phiPT1 belonged...
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Elsevier
2023-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023002840 |
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| author | Mona G. Alharbi Rashad R. Al-Hindi Ibrahim A. Alotibi Sheren A. Azhari Reem M. Farsi Addisu D. Teklemariam |
| author_facet | Mona G. Alharbi Rashad R. Al-Hindi Ibrahim A. Alotibi Sheren A. Azhari Reem M. Farsi Addisu D. Teklemariam |
| author_sort | Mona G. Alharbi |
| collection | DOAJ |
| description | Foodborne infections caused by Salmonella spp. are among the most common foodborne diseases in the world. We isolated a lytic phage against extended-spectrum beta-lactam producing S. Enteritidis strain PT1 derived from chicken carcass. Results from electronmicrography indicated that phiPT1 belonged to the family, Siphoviridae, in the order, Caudovirales. Phage phiPT1 was stable at temperatures from 4 °C to 60 °C and inactivated at 90 °C. phiPT1 retained a high titer from pH 4 to pH 10 for at least 1 h. Nevertheless, it displayed a significant decrease (p < 0.05) in titer at pH 11 and 12, with phage titers of 5.5 and 2.4 log10 PFU/mL, respectively. The latent time and burst size of phiPT1 were estimated to be 30 min and 252 PFU/infected cell, respectively. The virulence of phage phiPT1 was evaluated against S. Enteritidis strain PT1 at different MOIs. phiPT1 reduced Salmonella proliferation relative to the negative control (MOI 0) at all MOIs (P < 0.05). However, there is no significant difference among the MOIs (P > 0.05). The phage-antibiotic combination analysis (PAS) indicated that synergism was not detected at higher phiPT1 titer (1012 PFU/mL) with all tested antibiotics at all subinhibitory concentrations. However, synergistic activities were recorded at 0.25 × MIC of four tested antibiotics: cefixime, gentamicin, ciprofloxacin, and aztreonam in combination with phage at 104, 106 and 108 PFU/mL (ΣFIC ≤0.5). Synergism was detected for all antibiotics (0.1 × MIC) except meropenem and colistin in combination with phiPT1 at 104, 106 and 108 PFU/mL (ΣFIC ≤0.5). Synergism also displayed at the lowest concentrations of all antibiotics (0.01 MIC) in combination with phiPT1 at all titers except 1012 PFU/mL. Such characteristic features make phiPT1 to be a potential candidate for therapeutic uses. |
| first_indexed | 2024-04-10T17:45:53Z |
| format | Article |
| id | doaj.art-d9a8abceb70f41be9601e7b1e6c5385b |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-10T17:45:53Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-d9a8abceb70f41be9601e7b1e6c5385b2023-02-03T05:00:24ZengElsevierHeliyon2405-84402023-01-0191e13077Evaluation of phage—antibiotic combinations in the treatment of extended-spectrum β-lactamase-producing Salmonella enteritidis strain PT1Mona G. Alharbi0Rashad R. Al-Hindi1Ibrahim A. Alotibi2Sheren A. Azhari3Reem M. Farsi4Addisu D. Teklemariam5Department of Biological Sciences, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaDepartment of Biological Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; Corresponding author.Health Information Technology Department, Applied College, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaDepartment of Biological Sciences, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaDepartment of Biological Sciences, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaDepartment of Biological Sciences, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaFoodborne infections caused by Salmonella spp. are among the most common foodborne diseases in the world. We isolated a lytic phage against extended-spectrum beta-lactam producing S. Enteritidis strain PT1 derived from chicken carcass. Results from electronmicrography indicated that phiPT1 belonged to the family, Siphoviridae, in the order, Caudovirales. Phage phiPT1 was stable at temperatures from 4 °C to 60 °C and inactivated at 90 °C. phiPT1 retained a high titer from pH 4 to pH 10 for at least 1 h. Nevertheless, it displayed a significant decrease (p < 0.05) in titer at pH 11 and 12, with phage titers of 5.5 and 2.4 log10 PFU/mL, respectively. The latent time and burst size of phiPT1 were estimated to be 30 min and 252 PFU/infected cell, respectively. The virulence of phage phiPT1 was evaluated against S. Enteritidis strain PT1 at different MOIs. phiPT1 reduced Salmonella proliferation relative to the negative control (MOI 0) at all MOIs (P < 0.05). However, there is no significant difference among the MOIs (P > 0.05). The phage-antibiotic combination analysis (PAS) indicated that synergism was not detected at higher phiPT1 titer (1012 PFU/mL) with all tested antibiotics at all subinhibitory concentrations. However, synergistic activities were recorded at 0.25 × MIC of four tested antibiotics: cefixime, gentamicin, ciprofloxacin, and aztreonam in combination with phage at 104, 106 and 108 PFU/mL (ΣFIC ≤0.5). Synergism was detected for all antibiotics (0.1 × MIC) except meropenem and colistin in combination with phiPT1 at 104, 106 and 108 PFU/mL (ΣFIC ≤0.5). Synergism also displayed at the lowest concentrations of all antibiotics (0.01 MIC) in combination with phiPT1 at all titers except 1012 PFU/mL. Such characteristic features make phiPT1 to be a potential candidate for therapeutic uses.http://www.sciencedirect.com/science/article/pii/S2405844023002840Extended-spectrum beta-lactamasePhage antibiotic synergismSiphoviridae phageSalmonella enteritidisWastewater |
| spellingShingle | Mona G. Alharbi Rashad R. Al-Hindi Ibrahim A. Alotibi Sheren A. Azhari Reem M. Farsi Addisu D. Teklemariam Evaluation of phage—antibiotic combinations in the treatment of extended-spectrum β-lactamase-producing Salmonella enteritidis strain PT1 Heliyon Extended-spectrum beta-lactamase Phage antibiotic synergism Siphoviridae phage Salmonella enteritidis Wastewater |
| title | Evaluation of phage—antibiotic combinations in the treatment of extended-spectrum β-lactamase-producing Salmonella enteritidis strain PT1 |
| title_full | Evaluation of phage—antibiotic combinations in the treatment of extended-spectrum β-lactamase-producing Salmonella enteritidis strain PT1 |
| title_fullStr | Evaluation of phage—antibiotic combinations in the treatment of extended-spectrum β-lactamase-producing Salmonella enteritidis strain PT1 |
| title_full_unstemmed | Evaluation of phage—antibiotic combinations in the treatment of extended-spectrum β-lactamase-producing Salmonella enteritidis strain PT1 |
| title_short | Evaluation of phage—antibiotic combinations in the treatment of extended-spectrum β-lactamase-producing Salmonella enteritidis strain PT1 |
| title_sort | evaluation of phage antibiotic combinations in the treatment of extended spectrum β lactamase producing salmonella enteritidis strain pt1 |
| topic | Extended-spectrum beta-lactamase Phage antibiotic synergism Siphoviridae phage Salmonella enteritidis Wastewater |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023002840 |
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