Identification and functional analysis of non-coding regulatory small RNA FenSr3 in Bacillus amyloliquefaciens LPB-18
Bacillus amyloliquefaciens is an interesting microbe in the food processing and manufacturing industries. Non-coding small RNAs (sRNAs) have been shown to play a crucial role in the physiology and metabolism of bacteria by post-transcriptionally regulating gene expression. This study investigated th...
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PeerJ Inc.
2023-05-01
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| Online Access: | https://peerj.com/articles/15236.pdf |
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| author | Panping Yang Chengxin Geng Shaohui Zhu Zhen Zhou Muhammad Bilal Chengyuan Gu Hai Xu Linchun Ji Benchang Xiao Jingye Wang Zhoujie Qian Li Zhao Yuping Zhao Hedong Lu |
| author_facet | Panping Yang Chengxin Geng Shaohui Zhu Zhen Zhou Muhammad Bilal Chengyuan Gu Hai Xu Linchun Ji Benchang Xiao Jingye Wang Zhoujie Qian Li Zhao Yuping Zhao Hedong Lu |
| author_sort | Panping Yang |
| collection | DOAJ |
| description | Bacillus amyloliquefaciens is an interesting microbe in the food processing and manufacturing industries. Non-coding small RNAs (sRNAs) have been shown to play a crucial role in the physiology and metabolism of bacteria by post-transcriptionally regulating gene expression. This study investigated the function of novel sRNA FenSr3 by constructing fenSr3 deficient strain and complementary strains in B. amyloliquefaciens LPB-18 , which were named LPN-18N and LPB-18P, respectively. The result showed significant differences in fengycin yield between strain LPB -18N and LPB-18P. The production of fengycin was significantly enhanced in B. amyloliquefaciens LPB-18N, compared with that of the strain LPB-18 from 190.908 mg/L to 327.598 mg/L. Moreover, the production of fengycin decreased from 190.464 mg/L to 38.6 mg/L in B . amyloliquefaciens LPB-18P. A comparative transcriptome sequencing was carried out to better understand the complex regulatory mechanism. Transcription analysis revealed that 1037 genes were differentially expressed between B. amyloliquefaciens LPB-18 and B. amyloliquefaciens LPB-18N, including the key regulatory genes in fatty acid, amino acid biosynthesis, and central carbon metabolism, which could provide sufficient quantities of building precursors for fengycin biosynthesis. The biofilm formation and sporulation was also enhanced in the strain LPB-18N, which indicates that FenSr3 could play a vital role in stress resistance and promotes survival in B. amyloliquefaciens. Some sRNAs involved in stress response have been identified in the literature, but their regulatory roles in fengycin production remain unclear. The study will contribute a novel perspective to the regulation mechanism of biosynthesis and the optimization of key metabolites of B. amyloliquefaciens. |
| first_indexed | 2024-03-09T06:29:21Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:29:21Z |
| publishDate | 2023-05-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-d9a9d1f0219e4f749c1f6b9dc8788f772023-12-03T11:13:14ZengPeerJ Inc.PeerJ2167-83592023-05-0111e1523610.7717/peerj.15236Identification and functional analysis of non-coding regulatory small RNA FenSr3 in Bacillus amyloliquefaciens LPB-18Panping Yang0Chengxin Geng1Shaohui Zhu2Zhen Zhou3Muhammad Bilal4Chengyuan Gu5Hai Xu6Linchun Ji7Benchang Xiao8Jingye Wang9Zhoujie Qian10Li Zhao11Yuping Zhao12Hedong Lu13School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaiyin Institute of Technology, Huaian, Jiangsu, ChinaBacillus amyloliquefaciens is an interesting microbe in the food processing and manufacturing industries. Non-coding small RNAs (sRNAs) have been shown to play a crucial role in the physiology and metabolism of bacteria by post-transcriptionally regulating gene expression. This study investigated the function of novel sRNA FenSr3 by constructing fenSr3 deficient strain and complementary strains in B. amyloliquefaciens LPB-18 , which were named LPN-18N and LPB-18P, respectively. The result showed significant differences in fengycin yield between strain LPB -18N and LPB-18P. The production of fengycin was significantly enhanced in B. amyloliquefaciens LPB-18N, compared with that of the strain LPB-18 from 190.908 mg/L to 327.598 mg/L. Moreover, the production of fengycin decreased from 190.464 mg/L to 38.6 mg/L in B . amyloliquefaciens LPB-18P. A comparative transcriptome sequencing was carried out to better understand the complex regulatory mechanism. Transcription analysis revealed that 1037 genes were differentially expressed between B. amyloliquefaciens LPB-18 and B. amyloliquefaciens LPB-18N, including the key regulatory genes in fatty acid, amino acid biosynthesis, and central carbon metabolism, which could provide sufficient quantities of building precursors for fengycin biosynthesis. The biofilm formation and sporulation was also enhanced in the strain LPB-18N, which indicates that FenSr3 could play a vital role in stress resistance and promotes survival in B. amyloliquefaciens. Some sRNAs involved in stress response have been identified in the literature, but their regulatory roles in fengycin production remain unclear. The study will contribute a novel perspective to the regulation mechanism of biosynthesis and the optimization of key metabolites of B. amyloliquefaciens.https://peerj.com/articles/15236.pdfFengycin Biosynthesis sRNA Transcriptome Post-transcriptional regulation |
| spellingShingle | Panping Yang Chengxin Geng Shaohui Zhu Zhen Zhou Muhammad Bilal Chengyuan Gu Hai Xu Linchun Ji Benchang Xiao Jingye Wang Zhoujie Qian Li Zhao Yuping Zhao Hedong Lu Identification and functional analysis of non-coding regulatory small RNA FenSr3 in Bacillus amyloliquefaciens LPB-18 PeerJ Fengycin Biosynthesis sRNA Transcriptome Post-transcriptional regulation |
| title | Identification and functional analysis of non-coding regulatory small RNA FenSr3 in Bacillus amyloliquefaciens LPB-18 |
| title_full | Identification and functional analysis of non-coding regulatory small RNA FenSr3 in Bacillus amyloliquefaciens LPB-18 |
| title_fullStr | Identification and functional analysis of non-coding regulatory small RNA FenSr3 in Bacillus amyloliquefaciens LPB-18 |
| title_full_unstemmed | Identification and functional analysis of non-coding regulatory small RNA FenSr3 in Bacillus amyloliquefaciens LPB-18 |
| title_short | Identification and functional analysis of non-coding regulatory small RNA FenSr3 in Bacillus amyloliquefaciens LPB-18 |
| title_sort | identification and functional analysis of non coding regulatory small rna fensr3 in bacillus amyloliquefaciens lpb 18 |
| topic | Fengycin Biosynthesis sRNA Transcriptome Post-transcriptional regulation |
| url | https://peerj.com/articles/15236.pdf |
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