Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in <i>Rhododendron tomentosum</i> Extracts and Their Antioxidant and Antimicrobial Activity
In the literature, the chemical composition of <i>Rhododendron tomentosum</i> is mainly represented by the study of isoprenoid compounds of essential oil. In contrast, the study of the content of flavonoids will contribute to the expansion of pharmacological action and the use of the med...
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MDPI AG
2024-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/5/1095 |
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| author | Halyna Kukhtenko Nataliia Bevz Yulian Konechnyi Oleksandr Kukhtenko Izabela Jasicka-Misiak |
| author_facet | Halyna Kukhtenko Nataliia Bevz Yulian Konechnyi Oleksandr Kukhtenko Izabela Jasicka-Misiak |
| author_sort | Halyna Kukhtenko |
| collection | DOAJ |
| description | In the literature, the chemical composition of <i>Rhododendron tomentosum</i> is mainly represented by the study of isoprenoid compounds of essential oil. In contrast, the study of the content of flavonoids will contribute to the expansion of pharmacological action and the use of the medicinal plant for medical purposes. The paper deals with the technology of extracts from <i>Rh. tomentosum</i> shoots using ethanol of various concentrations and purified water as an extractant. Extracts from <i>Rh. tomentosum</i> were obtained by a modified method that combined the effects of ultrasound and temperature to maximize the extraction of biologically active substances from the raw material. Using the method of high-performance thin-layer chromatography in a system with solvents ethyl acetate/formic acid/water (15:1:1), the following substances have been separated and identified in all the extracts obtained: rutin, hyperoside, quercetin, and chlorogenic acid. The total polyphenol content (TPC) and total flavonoid content (TFC) were estimated using spectrophotometric methods involving the Folin-Ciocalteu (F-C) reagent and the complexation reaction with aluminum chloride, respectively. A correlation analysis was conducted between antioxidant activity and the polyphenolic substance content. Following the DPPH assay, regression analysis shows that phenolic compounds contribute to about 80% (<i>r</i><sup>2</sup> = 0.8028, <i>p</i> < 0.05) of radical scavenging properties in the extract of <i>Rh. tomentosum</i>. The extract of <i>Rh. tomentosum</i> obtained by ethanol 30% inhibits the growth of test cultures of microorganisms in 1:1 and 1:2 dilutions of the clinical strains #211 <i>Staphylococcus aureus</i> and #222 <i>Enterococcus</i> spp. and the reference strain <i>Pseudomonas aeruginosa</i> ATCC 10145. |
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| language | English |
| last_indexed | 2024-04-25T00:23:48Z |
| publishDate | 2024-02-01 |
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| spelling | doaj.art-d9aa70c5435942a8b0817565cbc4be7b2024-03-12T16:51:03ZengMDPI AGMolecules1420-30492024-02-01295109510.3390/molecules29051095Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in <i>Rhododendron tomentosum</i> Extracts and Their Antioxidant and Antimicrobial ActivityHalyna Kukhtenko0Nataliia Bevz1Yulian Konechnyi2Oleksandr Kukhtenko3Izabela Jasicka-Misiak4Institute of Chemistry, University of Opole, 48 Oleska Str., 45-052 Opole, PolandDepartment of Pharmaceutical Chemistry, National University of Pharmacy, 53 Pushkinska Str., 61002 Kharkiv, UkraineDepartment of Microbiology, Danylo Halytsky Lviv National Medical University, 69 Pekarska, 79010 Lviv, UkraineDepartment of Technology of Pharmaceutical Preparations, National University of Pharmacy, 53 Pushkinska Str., 61002 Kharkiv, UkraineInstitute of Chemistry, University of Opole, 48 Oleska Str., 45-052 Opole, PolandIn the literature, the chemical composition of <i>Rhododendron tomentosum</i> is mainly represented by the study of isoprenoid compounds of essential oil. In contrast, the study of the content of flavonoids will contribute to the expansion of pharmacological action and the use of the medicinal plant for medical purposes. The paper deals with the technology of extracts from <i>Rh. tomentosum</i> shoots using ethanol of various concentrations and purified water as an extractant. Extracts from <i>Rh. tomentosum</i> were obtained by a modified method that combined the effects of ultrasound and temperature to maximize the extraction of biologically active substances from the raw material. Using the method of high-performance thin-layer chromatography in a system with solvents ethyl acetate/formic acid/water (15:1:1), the following substances have been separated and identified in all the extracts obtained: rutin, hyperoside, quercetin, and chlorogenic acid. The total polyphenol content (TPC) and total flavonoid content (TFC) were estimated using spectrophotometric methods involving the Folin-Ciocalteu (F-C) reagent and the complexation reaction with aluminum chloride, respectively. A correlation analysis was conducted between antioxidant activity and the polyphenolic substance content. Following the DPPH assay, regression analysis shows that phenolic compounds contribute to about 80% (<i>r</i><sup>2</sup> = 0.8028, <i>p</i> < 0.05) of radical scavenging properties in the extract of <i>Rh. tomentosum</i>. The extract of <i>Rh. tomentosum</i> obtained by ethanol 30% inhibits the growth of test cultures of microorganisms in 1:1 and 1:2 dilutions of the clinical strains #211 <i>Staphylococcus aureus</i> and #222 <i>Enterococcus</i> spp. and the reference strain <i>Pseudomonas aeruginosa</i> ATCC 10145.https://www.mdpi.com/1420-3049/29/5/1095<i>Rhododendron tomentosum</i><i>Ledum palustre</i>rutinhyperosidequercetinchlorogenic acid |
| spellingShingle | Halyna Kukhtenko Nataliia Bevz Yulian Konechnyi Oleksandr Kukhtenko Izabela Jasicka-Misiak Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in <i>Rhododendron tomentosum</i> Extracts and Their Antioxidant and Antimicrobial Activity Molecules <i>Rhododendron tomentosum</i> <i>Ledum palustre</i> rutin hyperoside quercetin chlorogenic acid |
| title | Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in <i>Rhododendron tomentosum</i> Extracts and Their Antioxidant and Antimicrobial Activity |
| title_full | Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in <i>Rhododendron tomentosum</i> Extracts and Their Antioxidant and Antimicrobial Activity |
| title_fullStr | Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in <i>Rhododendron tomentosum</i> Extracts and Their Antioxidant and Antimicrobial Activity |
| title_full_unstemmed | Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in <i>Rhododendron tomentosum</i> Extracts and Their Antioxidant and Antimicrobial Activity |
| title_short | Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in <i>Rhododendron tomentosum</i> Extracts and Their Antioxidant and Antimicrobial Activity |
| title_sort | spectrophotometric and chromatographic assessment of total polyphenol and flavonoid content in i rhododendron tomentosum i extracts and their antioxidant and antimicrobial activity |
| topic | <i>Rhododendron tomentosum</i> <i>Ledum palustre</i> rutin hyperoside quercetin chlorogenic acid |
| url | https://www.mdpi.com/1420-3049/29/5/1095 |
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