The genome sequence of the colonial chordate, Botryllus schlosseri
Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relative...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2013-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/00569 |
| _version_ | 1811180502168633344 |
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| author | Ayelet Voskoboynik Norma F Neff Debashis Sahoo Aaron M Newman Dmitry Pushkarev Winston Koh Benedetto Passarelli H Christina Fan Gary L Mantalas Karla J Palmeri Katherine J Ishizuka Carmela Gissi Francesca Griggio Rachel Ben-Shlomo Daniel M Corey Lolita Penland Richard A White III Irving L Weissman Stephen R Quake |
| author_facet | Ayelet Voskoboynik Norma F Neff Debashis Sahoo Aaron M Newman Dmitry Pushkarev Winston Koh Benedetto Passarelli H Christina Fan Gary L Mantalas Karla J Palmeri Katherine J Ishizuka Carmela Gissi Francesca Griggio Rachel Ben-Shlomo Daniel M Corey Lolita Penland Richard A White III Irving L Weissman Stephen R Quake |
| author_sort | Ayelet Voskoboynik |
| collection | DOAJ |
| description | Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confidently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration. |
| first_indexed | 2024-04-11T09:04:18Z |
| format | Article |
| id | doaj.art-d9ad088483c9498798eee44a0828c694 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:04:18Z |
| publishDate | 2013-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-d9ad088483c9498798eee44a0828c6942022-12-22T04:32:41ZengeLife Sciences Publications LtdeLife2050-084X2013-07-01210.7554/eLife.00569The genome sequence of the colonial chordate, Botryllus schlosseriAyelet Voskoboynik0Norma F Neff1Debashis Sahoo2Aaron M Newman3Dmitry Pushkarev4Winston Koh5Benedetto Passarelli6H Christina Fan7Gary L Mantalas8Karla J Palmeri9Katherine J Ishizuka10Carmela Gissi11Francesca Griggio12Rachel Ben-Shlomo13Daniel M Corey14Lolita Penland15Richard A White III16Irving L Weissman17Stephen R Quake18Department of Pathology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, United States; Hopkins Marine Station, Stanford University, Pacific Grove, United StatesDepartments of Applied Physics and Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartment of Pathology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, United StatesDepartment of Pathology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, United StatesDepartments of Applied Physics and Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartments of Applied Physics and Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartments of Applied Physics and Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartments of Applied Physics and Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartments of Applied Physics and Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartment of Pathology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, United States; Hopkins Marine Station, Stanford University, Pacific Grove, United StatesDepartment of Pathology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, United States; Hopkins Marine Station, Stanford University, Pacific Grove, United StatesDipartimento di Bioscienze, Università degli Studi di Milano, Milano, ItalyDipartimento di Bioscienze, Università degli Studi di Milano, Milano, ItalyDepartment of Biology, University of Haifa-Oranim, Tivon, IsraelDepartment of Pathology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, United StatesDepartments of Applied Physics and Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartments of Applied Physics and Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesDepartment of Pathology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, United States; Hopkins Marine Station, Stanford University, Pacific Grove, United States; Ludwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, United StatesDepartments of Applied Physics and Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, United StatesBotryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confidently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration.https://elifesciences.org/articles/00569Botryllus schlosseritunicatestem cellhematopoiesisvertebrate evolutiongenome |
| spellingShingle | Ayelet Voskoboynik Norma F Neff Debashis Sahoo Aaron M Newman Dmitry Pushkarev Winston Koh Benedetto Passarelli H Christina Fan Gary L Mantalas Karla J Palmeri Katherine J Ishizuka Carmela Gissi Francesca Griggio Rachel Ben-Shlomo Daniel M Corey Lolita Penland Richard A White III Irving L Weissman Stephen R Quake The genome sequence of the colonial chordate, Botryllus schlosseri eLife Botryllus schlosseri tunicate stem cell hematopoiesis vertebrate evolution genome |
| title | The genome sequence of the colonial chordate, Botryllus schlosseri |
| title_full | The genome sequence of the colonial chordate, Botryllus schlosseri |
| title_fullStr | The genome sequence of the colonial chordate, Botryllus schlosseri |
| title_full_unstemmed | The genome sequence of the colonial chordate, Botryllus schlosseri |
| title_short | The genome sequence of the colonial chordate, Botryllus schlosseri |
| title_sort | genome sequence of the colonial chordate botryllus schlosseri |
| topic | Botryllus schlosseri tunicate stem cell hematopoiesis vertebrate evolution genome |
| url | https://elifesciences.org/articles/00569 |
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