Effects of Low Frequency Stimulation on Spontaneous Inhibitory and Excitatory Post-Synaptic Currents in Hippocampal CA1 Pyramidal Cells of Kindled Rats
Objective: Low-frequency stimulation (LFS) exerts suppressive effects in kindled animals. It is believed that overstimulated glutamatergic and decreased GABAergic transmission have long been associated with seizure activity. In this study, we investigated the effect of electrical LFS on different...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2016-10-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/journal/article/6878/download |
| _version_ | 1819182751253790720 |
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| author | Samireh Ghafouri, Yaghoub Fathollahi Saeed Semnanian Amir Shojaei Javad Mirnajafi-Zadeh |
| author_facet | Samireh Ghafouri, Yaghoub Fathollahi Saeed Semnanian Amir Shojaei Javad Mirnajafi-Zadeh |
| author_sort | Samireh Ghafouri, |
| collection | DOAJ |
| description | Objective: Low-frequency stimulation (LFS) exerts suppressive effects in kindled animals. It
is believed that overstimulated glutamatergic and decreased GABAergic transmission have
long been associated with seizure activity. In this study, we investigated the effect of electrical
LFS on different parameters of spontaneous excitatory and inhibitory post-synaptic currents
(sEPSCs and sIPSCs) in hippocampal CA1 pyramidal cells in kindled animals.
Materials and Methods: In this experimental study, rats were kindled by electrical stimulation
of the hippocampal CA1 area in a semi-rapid manner (12 stimulations/day). The
animals were considered fully kindled when they showed stage 5 seizures on three consecutive
days. One group of animals received LFS 4 times at 30 seconds, 6 hours, 18 and
24 hours following the last kindling stimulation. Each LFS consisted of 4 packages at 5
minutes intervals. Each package of LFS consisted of 200 pulses at 1 Hz and each monophasic
square wave pulse duration was 0.1 millisecond. At 2-3 hours post-LFS, acute
hippocampal slices were prepared and a whole cell patch clamp recording was performed
in all animals to measure the different parameters of sEPSCs and sIPSCs.
Results: In kindled animals, the inter-event interval (as an index of occurrence) of sEPSCs
decreased, whereas sIPSC increased. In addition, the decay time constant of sIPSCs
as an index of the duration of its activity decreased compared to the control group. There
was no significant difference in other parameters between the kindled and control groups.
Application of LFS in kindled animals prevented the observed changes. There was no significant
difference between the measured parameters in kindled+LFS and control groups.
Conclusion: LFS application may prevent seizure-induced increase in the occurrence
of sEPSCs and seizure-induced decrease in occurrence and activity duration of sIPSCs. |
| first_indexed | 2024-12-22T22:51:06Z |
| format | Article |
| id | doaj.art-d9b0bc355b9c4eccac32f73536099c1d |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-22T22:51:06Z |
| publishDate | 2016-10-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-d9b0bc355b9c4eccac32f73536099c1d2022-12-21T18:09:58ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142016-10-01184547555Effects of Low Frequency Stimulation on Spontaneous Inhibitory and Excitatory Post-Synaptic Currents in Hippocampal CA1 Pyramidal Cells of Kindled RatsSamireh Ghafouri,0Yaghoub Fathollahi1Saeed Semnanian2Amir Shojaei3Javad Mirnajafi-Zadeh4Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranObjective: Low-frequency stimulation (LFS) exerts suppressive effects in kindled animals. It is believed that overstimulated glutamatergic and decreased GABAergic transmission have long been associated with seizure activity. In this study, we investigated the effect of electrical LFS on different parameters of spontaneous excitatory and inhibitory post-synaptic currents (sEPSCs and sIPSCs) in hippocampal CA1 pyramidal cells in kindled animals. Materials and Methods: In this experimental study, rats were kindled by electrical stimulation of the hippocampal CA1 area in a semi-rapid manner (12 stimulations/day). The animals were considered fully kindled when they showed stage 5 seizures on three consecutive days. One group of animals received LFS 4 times at 30 seconds, 6 hours, 18 and 24 hours following the last kindling stimulation. Each LFS consisted of 4 packages at 5 minutes intervals. Each package of LFS consisted of 200 pulses at 1 Hz and each monophasic square wave pulse duration was 0.1 millisecond. At 2-3 hours post-LFS, acute hippocampal slices were prepared and a whole cell patch clamp recording was performed in all animals to measure the different parameters of sEPSCs and sIPSCs. Results: In kindled animals, the inter-event interval (as an index of occurrence) of sEPSCs decreased, whereas sIPSC increased. In addition, the decay time constant of sIPSCs as an index of the duration of its activity decreased compared to the control group. There was no significant difference in other parameters between the kindled and control groups. Application of LFS in kindled animals prevented the observed changes. There was no significant difference between the measured parameters in kindled+LFS and control groups. Conclusion: LFS application may prevent seizure-induced increase in the occurrence of sEPSCs and seizure-induced decrease in occurrence and activity duration of sIPSCs.http://celljournal.org/journal/article/6878/downloadSeizurePost Synaptic PotentialLow-Frequency StimulationKindling |
| spellingShingle | Samireh Ghafouri, Yaghoub Fathollahi Saeed Semnanian Amir Shojaei Javad Mirnajafi-Zadeh Effects of Low Frequency Stimulation on Spontaneous Inhibitory and Excitatory Post-Synaptic Currents in Hippocampal CA1 Pyramidal Cells of Kindled Rats Cell Journal Seizure Post Synaptic Potential Low-Frequency Stimulation Kindling |
| title | Effects of Low Frequency Stimulation on Spontaneous Inhibitory and Excitatory Post-Synaptic Currents in Hippocampal CA1 Pyramidal Cells of Kindled Rats |
| title_full | Effects of Low Frequency Stimulation on Spontaneous Inhibitory and Excitatory Post-Synaptic Currents in Hippocampal CA1 Pyramidal Cells of Kindled Rats |
| title_fullStr | Effects of Low Frequency Stimulation on Spontaneous Inhibitory and Excitatory Post-Synaptic Currents in Hippocampal CA1 Pyramidal Cells of Kindled Rats |
| title_full_unstemmed | Effects of Low Frequency Stimulation on Spontaneous Inhibitory and Excitatory Post-Synaptic Currents in Hippocampal CA1 Pyramidal Cells of Kindled Rats |
| title_short | Effects of Low Frequency Stimulation on Spontaneous Inhibitory and Excitatory Post-Synaptic Currents in Hippocampal CA1 Pyramidal Cells of Kindled Rats |
| title_sort | effects of low frequency stimulation on spontaneous inhibitory and excitatory post synaptic currents in hippocampal ca1 pyramidal cells of kindled rats |
| topic | Seizure Post Synaptic Potential Low-Frequency Stimulation Kindling |
| url | http://celljournal.org/journal/article/6878/download |
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