Thin-Layer Film with an Incorporated Pyrazoloquinoline Derivative as a Fluorescent Sensor for Nucleotides
A thin-layer film comprising poly(methyl methacrylate) with an incorporated pyrazolo[3,4- b ]quinoline derivative was prepared. The dye was anchored onto the polymer film to form a molecular assembly at the surface. Fluorescence quenching of this thin-layer sensory system was observed when the film...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2004-11-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1260/0263617043026505 |
| _version_ | 1826905980849356800 |
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| author | Piotr Cywiński Barbara Wandelt Andrzej Danel |
| author_facet | Piotr Cywiński Barbara Wandelt Andrzej Danel |
| author_sort | Piotr Cywiński |
| collection | DOAJ |
| description | A thin-layer film comprising poly(methyl methacrylate) with an incorporated pyrazolo[3,4- b ]quinoline derivative was prepared. The dye was anchored onto the polymer film to form a molecular assembly at the surface. Fluorescence quenching of this thin-layer sensory system was observed when the film was incubated in the presence of the nucleotide guanosine-3′,5′-cyclic monophosphate. Steady-state and time-resolved fluorescence techniques were used to study the fluorescence quenching of this sensory system in the presence of nucleotides. The results suggest that quenching of a pyrazoloquinoline fluorosensor by nucleotide anions cannot be described simply by a collision mechanism employing the Stern-Volmer equation, but there is a possibility of the formation of a more stable association. This type of sensing polymer layer plays a role in biotechnology because it can be immobilized at the end of a fibre optic and used for measuring nucleotide concentrations within a biological system. |
| first_indexed | 2024-03-07T17:31:28Z |
| format | Article |
| id | doaj.art-d9b1ff38bbd647eaa213d5f5e2c2aedd |
| institution | Directory Open Access Journal |
| issn | 0263-6174 2048-4038 |
| language | English |
| last_indexed | 2025-02-17T08:39:16Z |
| publishDate | 2004-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj.art-d9b1ff38bbd647eaa213d5f5e2c2aedd2025-01-02T21:31:20ZengSAGE PublishingAdsorption Science & Technology0263-61742048-40382004-11-012210.1260/0263617043026505Thin-Layer Film with an Incorporated Pyrazoloquinoline Derivative as a Fluorescent Sensor for NucleotidesPiotr Cywiński0Barbara Wandelt1Andrzej Danel2 Department of Molecular Physics, Faculty of Chemistry, Technical University of Lodz, Zeromskiego 116, Lodz 90 924, Poland Department of Molecular Physics, Faculty of Chemistry, Technical University of Lodz, Zeromskiego 116, Lodz 90 924, Poland Department of Chemistry, Hugo Kollataj Agricultural University, Al. Mickiewicza 24/28, 30 059 Krakow, PolandA thin-layer film comprising poly(methyl methacrylate) with an incorporated pyrazolo[3,4- b ]quinoline derivative was prepared. The dye was anchored onto the polymer film to form a molecular assembly at the surface. Fluorescence quenching of this thin-layer sensory system was observed when the film was incubated in the presence of the nucleotide guanosine-3′,5′-cyclic monophosphate. Steady-state and time-resolved fluorescence techniques were used to study the fluorescence quenching of this sensory system in the presence of nucleotides. The results suggest that quenching of a pyrazoloquinoline fluorosensor by nucleotide anions cannot be described simply by a collision mechanism employing the Stern-Volmer equation, but there is a possibility of the formation of a more stable association. This type of sensing polymer layer plays a role in biotechnology because it can be immobilized at the end of a fibre optic and used for measuring nucleotide concentrations within a biological system.https://doi.org/10.1260/0263617043026505 |
| spellingShingle | Piotr Cywiński Barbara Wandelt Andrzej Danel Thin-Layer Film with an Incorporated Pyrazoloquinoline Derivative as a Fluorescent Sensor for Nucleotides Adsorption Science & Technology |
| title | Thin-Layer Film with an Incorporated Pyrazoloquinoline Derivative as a Fluorescent Sensor for Nucleotides |
| title_full | Thin-Layer Film with an Incorporated Pyrazoloquinoline Derivative as a Fluorescent Sensor for Nucleotides |
| title_fullStr | Thin-Layer Film with an Incorporated Pyrazoloquinoline Derivative as a Fluorescent Sensor for Nucleotides |
| title_full_unstemmed | Thin-Layer Film with an Incorporated Pyrazoloquinoline Derivative as a Fluorescent Sensor for Nucleotides |
| title_short | Thin-Layer Film with an Incorporated Pyrazoloquinoline Derivative as a Fluorescent Sensor for Nucleotides |
| title_sort | thin layer film with an incorporated pyrazoloquinoline derivative as a fluorescent sensor for nucleotides |
| url | https://doi.org/10.1260/0263617043026505 |
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