Interspecific Potato Breeding Lines Display Differential Colonization Patterns and Induced Defense Responses after Ralstonia solanacearum Infection
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay ba...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01424/full |
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| author | Virginia Ferreira María J. Pianzzola Francisco L. Vilaró Guillermo A. Galván María L. Tondo María L. Tondo María V. Rodriguez Elena G. Orellano Elena G. Orellano Marc Valls Marc Valls María I. Siri |
| author_facet | Virginia Ferreira María J. Pianzzola Francisco L. Vilaró Guillermo A. Galván María L. Tondo María L. Tondo María V. Rodriguez Elena G. Orellano Elena G. Orellano Marc Valls Marc Valls María I. Siri |
| author_sort | Virginia Ferreira |
| collection | DOAJ |
| description | Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato. |
| first_indexed | 2024-04-12T20:38:44Z |
| format | Article |
| id | doaj.art-d9b21a05836a44ed8136d17069c8393a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T20:38:44Z |
| publishDate | 2017-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-d9b21a05836a44ed8136d17069c8393a2022-12-22T03:17:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-08-01810.3389/fpls.2017.01424291944Interspecific Potato Breeding Lines Display Differential Colonization Patterns and Induced Defense Responses after Ralstonia solanacearum InfectionVirginia Ferreira0María J. Pianzzola1Francisco L. Vilaró2Guillermo A. Galván3María L. Tondo4María L. Tondo5María V. Rodriguez6Elena G. Orellano7Elena G. Orellano8Marc Valls9Marc Valls10María I. Siri11Departamento de Biociencias, Facultad de Química, Universidad de la RepúblicaMontevideo, UruguayDepartamento de Biociencias, Facultad de Química, Universidad de la RepúblicaMontevideo, UruguayUnidad de Horticultura, INIA Las BrujasCanelones, UruguayDepartamento de Producción Vegetal, Centro Regional Sur, Facultad de Agronomía, Universidad de la RepúblicaCanelones, UruguayInstituto de Biología Molecular y Celular de Rosario (CONICET-UNR)Rosario, ArgentinaÁrea Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de RosarioRosario, ArgentinaÁrea Biología Vegetal (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de RosarioRosario, ArgentinaInstituto de Biología Molecular y Celular de Rosario (CONICET-UNR)Rosario, ArgentinaÁrea Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de RosarioRosario, ArgentinaCenter for Research in Agricultural Genomics, CSIC, IRTA, UAB, UBBarcelona, SpainDepartment of Genetics, Universitat de BarcelonaBarcelona, SpainDepartamento de Biociencias, Facultad de Química, Universidad de la RepúblicaMontevideo, UruguayPotato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato.http://journal.frontiersin.org/article/10.3389/fpls.2017.01424/fullbacterial wiltRalstonia solanacearumpotatoSolanum commersoniiplant breedingdisease resistance |
| spellingShingle | Virginia Ferreira María J. Pianzzola Francisco L. Vilaró Guillermo A. Galván María L. Tondo María L. Tondo María V. Rodriguez Elena G. Orellano Elena G. Orellano Marc Valls Marc Valls María I. Siri Interspecific Potato Breeding Lines Display Differential Colonization Patterns and Induced Defense Responses after Ralstonia solanacearum Infection Frontiers in Plant Science bacterial wilt Ralstonia solanacearum potato Solanum commersonii plant breeding disease resistance |
| title | Interspecific Potato Breeding Lines Display Differential Colonization Patterns and Induced Defense Responses after Ralstonia solanacearum Infection |
| title_full | Interspecific Potato Breeding Lines Display Differential Colonization Patterns and Induced Defense Responses after Ralstonia solanacearum Infection |
| title_fullStr | Interspecific Potato Breeding Lines Display Differential Colonization Patterns and Induced Defense Responses after Ralstonia solanacearum Infection |
| title_full_unstemmed | Interspecific Potato Breeding Lines Display Differential Colonization Patterns and Induced Defense Responses after Ralstonia solanacearum Infection |
| title_short | Interspecific Potato Breeding Lines Display Differential Colonization Patterns and Induced Defense Responses after Ralstonia solanacearum Infection |
| title_sort | interspecific potato breeding lines display differential colonization patterns and induced defense responses after ralstonia solanacearum infection |
| topic | bacterial wilt Ralstonia solanacearum potato Solanum commersonii plant breeding disease resistance |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01424/full |
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