Exploration of agr types, virulence−associated genes, and biofilm formation ability in Staphylococcus aureus isolates from hemodialysis patients with vascular access infections
IntroductionStaphylococcus aureus, is a pathogen commonly encountered in both community and hospital settings. Patients receiving hemodialysis treatment face an elevated risk of vascular access infections (VAIs) particularly Staphylococcus aureus, infection. This heightened risk is attributed to t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1367016/full |
| _version_ | 1811153555882508288 |
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| author | Chi-Hsiang Lai Min Yi Wong Tsung-Yu Huang Tsung-Yu Huang Chih-Chen Kao Chih-Chen Kao Yu-Hui Lin Chu-Hsueh Lu Yao-Kuang Huang Yao-Kuang Huang Yao-Kuang Huang Yao-Kuang Huang |
| author_facet | Chi-Hsiang Lai Min Yi Wong Tsung-Yu Huang Tsung-Yu Huang Chih-Chen Kao Chih-Chen Kao Yu-Hui Lin Chu-Hsueh Lu Yao-Kuang Huang Yao-Kuang Huang Yao-Kuang Huang Yao-Kuang Huang |
| author_sort | Chi-Hsiang Lai |
| collection | DOAJ |
| description | IntroductionStaphylococcus aureus, is a pathogen commonly encountered in both community and hospital settings. Patients receiving hemodialysis treatment face an elevated risk of vascular access infections (VAIs) particularly Staphylococcus aureus, infection. This heightened risk is attributed to the characteristics of Staphylococcus aureus, , enabling it to adhere to suitable surfaces and form biofilms, thereby rendering it resistant to external interventions and complicating treatment efforts.MethodsTherefore this study utilized PCR and microtiter dish biofilm formation assay to determine the difference in the virulence genes and biofilm formation among in our study collected of 103 Staphylococcus aureus, isolates from hemodialysis patients utilizing arteriovenous grafts (AVGs), tunneled cuffed catheters (TCCs), and arteriovenous fistulas (AVFs) during November 2013 to December 2021.ResultsOur findings revealed that both MRSA and MSSA isolates exhibited strong biofilm production capabilities. Additionally, we confirmed the presence of agr types and virulence genes through PCR analysis. The majority of the collected isolates were identified as agr type I. However, agr type II isolates displayed a higher average number of virulence genes, with MRSA isolates exhibiting a variety of virulence genes. Notably, combinations of biofilm-associated genes, such as eno−clfA−clfB−fib−icaA−icaD and eno−clfA−clfB−fib−fnbB−icaA−icaD, were prevalent among Staphylococcus aureus, isolates obtained from vascular access infections.DiscussionThese insights contribute to a better understanding of the molecular characteristics associated with Staphylococcus aureus, infections in hemodialysis patients and provided more targeted and effective treatment approaches. |
| first_indexed | 2024-04-24T10:59:03Z |
| format | Article |
| id | doaj.art-d9b236591b4442a4b2c04329a0ad9419 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-24T10:59:03Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d9b236591b4442a4b2c04329a0ad94192024-04-12T04:26:59ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-04-011410.3389/fcimb.2024.13670161367016Exploration of agr types, virulence−associated genes, and biofilm formation ability in Staphylococcus aureus isolates from hemodialysis patients with vascular access infectionsChi-Hsiang Lai0Min Yi Wong1Tsung-Yu Huang2Tsung-Yu Huang3Chih-Chen Kao4Chih-Chen Kao5Yu-Hui Lin6Chu-Hsueh Lu7Yao-Kuang Huang8Yao-Kuang Huang9Yao-Kuang Huang10Yao-Kuang Huang11Division of Thoracic and Cardiovascular Surgery, Chiayi Chang Gung Memorial Hospital, Chiayi, TaiwanDivision of Thoracic and Cardiovascular Surgery, Chiayi Chang Gung Memorial Hospital, Chiayi, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, Chiayi Chang Gung Memorial Hospital, Chiayi, TaiwanCollege of Medicine, Chang Gung University, Taoyuan, TaiwanDivision of Thoracic and Cardiovascular Surgery, Chiayi Chang Gung Memorial Hospital, Chiayi, TaiwanCollege of Medicine, Chang Gung University, Taoyuan, TaiwanDivision of Thoracic and Cardiovascular Surgery, Chiayi Chang Gung Memorial Hospital, Chiayi, TaiwanDivision of Thoracic and Cardiovascular Surgery, Chiayi Chang Gung Memorial Hospital, Chiayi, TaiwanDivision of Thoracic and Cardiovascular Surgery, Chiayi Chang Gung Memorial Hospital, Chiayi, TaiwanCollege of Medicine, Chang Gung University, Taoyuan, TaiwanDivision of Cardiovascular Surgery, New Taipei Municipal TuCheng Hospital, New Taipei, TaiwanDivision of Thoracic and Cardiovascular Surgery, Chiayi Hospital, Ministry of Health and Welfare, Chiayi, TaiwanIntroductionStaphylococcus aureus, is a pathogen commonly encountered in both community and hospital settings. Patients receiving hemodialysis treatment face an elevated risk of vascular access infections (VAIs) particularly Staphylococcus aureus, infection. This heightened risk is attributed to the characteristics of Staphylococcus aureus, , enabling it to adhere to suitable surfaces and form biofilms, thereby rendering it resistant to external interventions and complicating treatment efforts.MethodsTherefore this study utilized PCR and microtiter dish biofilm formation assay to determine the difference in the virulence genes and biofilm formation among in our study collected of 103 Staphylococcus aureus, isolates from hemodialysis patients utilizing arteriovenous grafts (AVGs), tunneled cuffed catheters (TCCs), and arteriovenous fistulas (AVFs) during November 2013 to December 2021.ResultsOur findings revealed that both MRSA and MSSA isolates exhibited strong biofilm production capabilities. Additionally, we confirmed the presence of agr types and virulence genes through PCR analysis. The majority of the collected isolates were identified as agr type I. However, agr type II isolates displayed a higher average number of virulence genes, with MRSA isolates exhibiting a variety of virulence genes. Notably, combinations of biofilm-associated genes, such as eno−clfA−clfB−fib−icaA−icaD and eno−clfA−clfB−fib−fnbB−icaA−icaD, were prevalent among Staphylococcus aureus, isolates obtained from vascular access infections.DiscussionThese insights contribute to a better understanding of the molecular characteristics associated with Staphylococcus aureus, infections in hemodialysis patients and provided more targeted and effective treatment approaches. https://www.frontiersin.org/articles/10.3389/fcimb.2024.1367016/fullvascular access infections (VAIs)Staphylococcus aureusAgr typingvirulence genesbiofilm formation abilitymolecular characterization |
| spellingShingle | Chi-Hsiang Lai Min Yi Wong Tsung-Yu Huang Tsung-Yu Huang Chih-Chen Kao Chih-Chen Kao Yu-Hui Lin Chu-Hsueh Lu Yao-Kuang Huang Yao-Kuang Huang Yao-Kuang Huang Yao-Kuang Huang Exploration of agr types, virulence−associated genes, and biofilm formation ability in Staphylococcus aureus isolates from hemodialysis patients with vascular access infections Frontiers in Cellular and Infection Microbiology vascular access infections (VAIs) Staphylococcus aureus Agr typing virulence genes biofilm formation ability molecular characterization |
| title | Exploration of agr types, virulence−associated genes, and biofilm formation ability in Staphylococcus aureus isolates from hemodialysis patients with vascular access infections |
| title_full | Exploration of agr types, virulence−associated genes, and biofilm formation ability in Staphylococcus aureus isolates from hemodialysis patients with vascular access infections |
| title_fullStr | Exploration of agr types, virulence−associated genes, and biofilm formation ability in Staphylococcus aureus isolates from hemodialysis patients with vascular access infections |
| title_full_unstemmed | Exploration of agr types, virulence−associated genes, and biofilm formation ability in Staphylococcus aureus isolates from hemodialysis patients with vascular access infections |
| title_short | Exploration of agr types, virulence−associated genes, and biofilm formation ability in Staphylococcus aureus isolates from hemodialysis patients with vascular access infections |
| title_sort | exploration of agr types virulence associated genes and biofilm formation ability in staphylococcus aureus isolates from hemodialysis patients with vascular access infections |
| topic | vascular access infections (VAIs) Staphylococcus aureus Agr typing virulence genes biofilm formation ability molecular characterization |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1367016/full |
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