The International Space Station Environment Triggers Molecular Responses in Aspergillus niger
Due to immense phenotypic plasticity and adaptability, Aspergillus niger is a cosmopolitan fungus that thrives in versatile environments, including the International Space Station (ISS). This is the first report of genomic, proteomic, and metabolomic alterations observed in A. niger strain JSC-09335...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.893071/full |
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| author | Adriana Blachowicz Adriana Blachowicz Jillian Romsdahl Abby J. Chiang Sawyer Masonjones Markus Kalkum Jason E. Stajich Tamas Torok Clay C. C. Wang Clay C. C. Wang Kasthuri Venkateswaran |
| author_facet | Adriana Blachowicz Adriana Blachowicz Jillian Romsdahl Abby J. Chiang Sawyer Masonjones Markus Kalkum Jason E. Stajich Tamas Torok Clay C. C. Wang Clay C. C. Wang Kasthuri Venkateswaran |
| author_sort | Adriana Blachowicz |
| collection | DOAJ |
| description | Due to immense phenotypic plasticity and adaptability, Aspergillus niger is a cosmopolitan fungus that thrives in versatile environments, including the International Space Station (ISS). This is the first report of genomic, proteomic, and metabolomic alterations observed in A. niger strain JSC-093350089 grown in a controlled experiment aboard the ISS. Whole-genome sequencing (WGS) revealed that ISS conditions, including microgravity and enhanced irradiation, triggered non-synonymous point mutations in specific regions, chromosomes VIII and XII of the JSC-093350089 genome when compared to the ground-grown control. Proteome analysis showed altered abundance of proteins involved in carbohydrate metabolism, stress response, and cellular amino acid and protein catabolic processes following growth aboard the ISS. Metabolome analysis further confirmed that space conditions altered molecular suite of ISS-grown A. niger JSC-093350089. After regrowing both strains on Earth, production of antioxidant—Pyranonigrin A was significantly induced in the ISS-flown, but not the ground control strain. In summary, the microgravity and enhanced irradiation triggered unique molecular responses in the A. niger JSC-093350089 suggesting adaptive responses. |
| first_indexed | 2024-04-12T12:25:53Z |
| format | Article |
| id | doaj.art-d9b2532468204cc38b39ebe035a8f2b4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T12:25:53Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d9b2532468204cc38b39ebe035a8f2b42022-12-22T03:33:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.893071893071The International Space Station Environment Triggers Molecular Responses in Aspergillus nigerAdriana Blachowicz0Adriana Blachowicz1Jillian Romsdahl2Abby J. Chiang3Sawyer Masonjones4Markus Kalkum5Jason E. Stajich6Tamas Torok7Clay C. C. Wang8Clay C. C. Wang9Kasthuri Venkateswaran10Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, United StatesBiotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United StatesDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, United StatesDepartment of Immunology and Theranostics, Beckman Research Institute of City of Hope, Duarte, CA, United StatesDepartment of Microbiology and Plant Pathology, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, United StatesDepartment of Immunology and Theranostics, Beckman Research Institute of City of Hope, Duarte, CA, United StatesDepartment of Microbiology and Plant Pathology, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, United StatesEcology Department, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, United StatesDepartment of Chemistry, Dornsife College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, CA, United StatesBiotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United StatesDue to immense phenotypic plasticity and adaptability, Aspergillus niger is a cosmopolitan fungus that thrives in versatile environments, including the International Space Station (ISS). This is the first report of genomic, proteomic, and metabolomic alterations observed in A. niger strain JSC-093350089 grown in a controlled experiment aboard the ISS. Whole-genome sequencing (WGS) revealed that ISS conditions, including microgravity and enhanced irradiation, triggered non-synonymous point mutations in specific regions, chromosomes VIII and XII of the JSC-093350089 genome when compared to the ground-grown control. Proteome analysis showed altered abundance of proteins involved in carbohydrate metabolism, stress response, and cellular amino acid and protein catabolic processes following growth aboard the ISS. Metabolome analysis further confirmed that space conditions altered molecular suite of ISS-grown A. niger JSC-093350089. After regrowing both strains on Earth, production of antioxidant—Pyranonigrin A was significantly induced in the ISS-flown, but not the ground control strain. In summary, the microgravity and enhanced irradiation triggered unique molecular responses in the A. niger JSC-093350089 suggesting adaptive responses.https://www.frontiersin.org/articles/10.3389/fmicb.2022.893071/fullAspergillus nigerInternational Space Stationmetabolomeproteomegenome |
| spellingShingle | Adriana Blachowicz Adriana Blachowicz Jillian Romsdahl Abby J. Chiang Sawyer Masonjones Markus Kalkum Jason E. Stajich Tamas Torok Clay C. C. Wang Clay C. C. Wang Kasthuri Venkateswaran The International Space Station Environment Triggers Molecular Responses in Aspergillus niger Frontiers in Microbiology Aspergillus niger International Space Station metabolome proteome genome |
| title | The International Space Station Environment Triggers Molecular Responses in Aspergillus niger |
| title_full | The International Space Station Environment Triggers Molecular Responses in Aspergillus niger |
| title_fullStr | The International Space Station Environment Triggers Molecular Responses in Aspergillus niger |
| title_full_unstemmed | The International Space Station Environment Triggers Molecular Responses in Aspergillus niger |
| title_short | The International Space Station Environment Triggers Molecular Responses in Aspergillus niger |
| title_sort | international space station environment triggers molecular responses in aspergillus niger |
| topic | Aspergillus niger International Space Station metabolome proteome genome |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.893071/full |
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