The COPII subunit MoSec24B is involved in development, pathogenicity and autophagy in the rice blast fungus
The endoplasmic reticulum (ER) acts as the starting point of the secretory pathway, where approximately one-third of the proteins are correctly folded and modified, loaded into vesicles, and transported to the Golgi for further processing and modification. In this process, COPII vesicles are respons...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1074107/full |
| _version_ | 1797956910719172608 |
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| author | Hui Qian Lixiao Sun Minghua Wu Wenhui Zhao Mengyu Liu Shuang Liang Xueming Zhu Lin Li Zhenzhu Su Jianping Lu Fucheng Lin Fucheng Lin Xiaohong Liu |
| author_facet | Hui Qian Lixiao Sun Minghua Wu Wenhui Zhao Mengyu Liu Shuang Liang Xueming Zhu Lin Li Zhenzhu Su Jianping Lu Fucheng Lin Fucheng Lin Xiaohong Liu |
| author_sort | Hui Qian |
| collection | DOAJ |
| description | The endoplasmic reticulum (ER) acts as the starting point of the secretory pathway, where approximately one-third of the proteins are correctly folded and modified, loaded into vesicles, and transported to the Golgi for further processing and modification. In this process, COPII vesicles are responsible for transporting cargo proteins from the ER to the Golgi. Here, we identified the inner shell subunit of COPII vesicles (MoSec24B) and explored the importance of MoSec24B in the rice blast fungus. The targeted disruption of MoSec24B led to decreased growth, reduced conidiation, restricted glycogen and lipids utilization, sensitivity to the cell wall and hypertonic stress, the failure of septin-mediated repolarization of appressorium, impaired appressorium turgor pressure, and decreased ability to infect, which resulted in reduced pathogenicity to the host plant. Furthermore, MoSec24B functions in the three mitogen-activated protein kinase (MAPK) signaling pathways by acting with MoMst50. Deletion of MoSec24B caused reduced lipidation of MoAtg8, accelerated degradation of exogenously introduced GFP-MoAtg8, and increased lipidation of MoAtg8 upon treatment with a late inhibitor of autophagy (BafA1), suggesting that MoSec24B regulates the fusion of late autophagosomes with vacuoles. Together, these results suggest that MoSec24B exerts a significant role in fungal development, the pathogenesis of filamentous fungi and autophagy. |
| first_indexed | 2024-04-10T23:57:11Z |
| format | Article |
| id | doaj.art-d9b909fe06874a59b3388a3eb58e1c12 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T23:57:11Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d9b909fe06874a59b3388a3eb58e1c122023-01-10T12:42:59ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10741071074107The COPII subunit MoSec24B is involved in development, pathogenicity and autophagy in the rice blast fungusHui Qian0Lixiao Sun1Minghua Wu2Wenhui Zhao3Mengyu Liu4Shuang Liang5Xueming Zhu6Lin Li7Zhenzhu Su8Jianping Lu9Fucheng Lin10Fucheng Lin11Xiaohong Liu12State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaCollege of Life Sciences, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaThe endoplasmic reticulum (ER) acts as the starting point of the secretory pathway, where approximately one-third of the proteins are correctly folded and modified, loaded into vesicles, and transported to the Golgi for further processing and modification. In this process, COPII vesicles are responsible for transporting cargo proteins from the ER to the Golgi. Here, we identified the inner shell subunit of COPII vesicles (MoSec24B) and explored the importance of MoSec24B in the rice blast fungus. The targeted disruption of MoSec24B led to decreased growth, reduced conidiation, restricted glycogen and lipids utilization, sensitivity to the cell wall and hypertonic stress, the failure of septin-mediated repolarization of appressorium, impaired appressorium turgor pressure, and decreased ability to infect, which resulted in reduced pathogenicity to the host plant. Furthermore, MoSec24B functions in the three mitogen-activated protein kinase (MAPK) signaling pathways by acting with MoMst50. Deletion of MoSec24B caused reduced lipidation of MoAtg8, accelerated degradation of exogenously introduced GFP-MoAtg8, and increased lipidation of MoAtg8 upon treatment with a late inhibitor of autophagy (BafA1), suggesting that MoSec24B regulates the fusion of late autophagosomes with vacuoles. Together, these results suggest that MoSec24B exerts a significant role in fungal development, the pathogenesis of filamentous fungi and autophagy.https://www.frontiersin.org/articles/10.3389/fpls.2022.1074107/fullCOPIISec24Magnaporthe oryzaeautophagypathogenicitysignaling pathway |
| spellingShingle | Hui Qian Lixiao Sun Minghua Wu Wenhui Zhao Mengyu Liu Shuang Liang Xueming Zhu Lin Li Zhenzhu Su Jianping Lu Fucheng Lin Fucheng Lin Xiaohong Liu The COPII subunit MoSec24B is involved in development, pathogenicity and autophagy in the rice blast fungus Frontiers in Plant Science COPII Sec24 Magnaporthe oryzae autophagy pathogenicity signaling pathway |
| title | The COPII subunit MoSec24B is involved in development, pathogenicity and autophagy in the rice blast fungus |
| title_full | The COPII subunit MoSec24B is involved in development, pathogenicity and autophagy in the rice blast fungus |
| title_fullStr | The COPII subunit MoSec24B is involved in development, pathogenicity and autophagy in the rice blast fungus |
| title_full_unstemmed | The COPII subunit MoSec24B is involved in development, pathogenicity and autophagy in the rice blast fungus |
| title_short | The COPII subunit MoSec24B is involved in development, pathogenicity and autophagy in the rice blast fungus |
| title_sort | copii subunit mosec24b is involved in development pathogenicity and autophagy in the rice blast fungus |
| topic | COPII Sec24 Magnaporthe oryzae autophagy pathogenicity signaling pathway |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1074107/full |
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