Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography
High-speed counter-current chromatography (HSCCC) is a major tool for the fast separation of natural products from plants. It was used for the preparative isolation of the flavonoid monoglucosides present in the aerial parts of the Davilla elliptica St. Hill. (Dilleniaceae). This species is used in...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Química
2006-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500011 |
| _version_ | 1819059358215962624 |
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| author | Rinaldo Daniel Silva Marcelo Aparecido Rodrigues Clenilson Martins Calvo Tamara Regina Sannomiya Miriam Santos Lourdes Campaner dos Vilegas Wagner Kushima Hélio Hiruma-Lima Clélia Akiko Brito Alba Regina Monteiro de Souza |
| author_facet | Rinaldo Daniel Silva Marcelo Aparecido Rodrigues Clenilson Martins Calvo Tamara Regina Sannomiya Miriam Santos Lourdes Campaner dos Vilegas Wagner Kushima Hélio Hiruma-Lima Clélia Akiko Brito Alba Regina Monteiro de Souza |
| author_sort | Rinaldo Daniel |
| collection | DOAJ |
| description | High-speed counter-current chromatography (HSCCC) is a major tool for the fast separation of natural products from plants. It was used for the preparative isolation of the flavonoid monoglucosides present in the aerial parts of the Davilla elliptica St. Hill. (Dilleniaceae). This species is used in Brazilian folk medicine for the treatment of gastric disorders. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80, v/v/v) and led to a successful separation of quercetin-3-O-alpha-L-rhamnopyranoside and myricetin-3-O-alpha-L-rhamnopyranoside in approximately 3.0 hours with purity higher than 95%. Identification was performed by ¹H NMR, 13C NMR and HPLC-UV-DAD analyses. |
| first_indexed | 2024-12-21T14:09:49Z |
| format | Article |
| id | doaj.art-d9b94ad2f4c94a65a3113e2af04cb782 |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-12-21T14:09:49Z |
| publishDate | 2006-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-d9b94ad2f4c94a65a3113e2af04cb7822022-12-21T19:01:05ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642006-01-01295947949Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatographyRinaldo DanielSilva Marcelo AparecidoRodrigues Clenilson MartinsCalvo Tamara ReginaSannomiya MiriamSantos Lourdes Campaner dosVilegas WagnerKushima HélioHiruma-Lima Clélia AkikoBrito Alba Regina Monteiro de SouzaHigh-speed counter-current chromatography (HSCCC) is a major tool for the fast separation of natural products from plants. It was used for the preparative isolation of the flavonoid monoglucosides present in the aerial parts of the Davilla elliptica St. Hill. (Dilleniaceae). This species is used in Brazilian folk medicine for the treatment of gastric disorders. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80, v/v/v) and led to a successful separation of quercetin-3-O-alpha-L-rhamnopyranoside and myricetin-3-O-alpha-L-rhamnopyranoside in approximately 3.0 hours with purity higher than 95%. Identification was performed by ¹H NMR, 13C NMR and HPLC-UV-DAD analyses.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500011HSCCCDavilla ellipticaflavonoids |
| spellingShingle | Rinaldo Daniel Silva Marcelo Aparecido Rodrigues Clenilson Martins Calvo Tamara Regina Sannomiya Miriam Santos Lourdes Campaner dos Vilegas Wagner Kushima Hélio Hiruma-Lima Clélia Akiko Brito Alba Regina Monteiro de Souza Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography Química Nova HSCCC Davilla elliptica flavonoids |
| title | Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography |
| title_full | Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography |
| title_fullStr | Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography |
| title_full_unstemmed | Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography |
| title_short | Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography |
| title_sort | preparative separation of flavonoids from the medicinal plant davilla elliptica st hill by high speed counter current chromatography |
| topic | HSCCC Davilla elliptica flavonoids |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500011 |
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