Molecular characterization and differential expression suggested diverse functions of P-type II Ca2+ATPases in Triticum aestivum L
Abstract Background Plant P-type II Ca2+ATPases are formed by two distinct groups of proteins (ACAs and ECAs) that perform pumping of Ca2+ outside the cytoplasm during homeostasis, and play vital functions during development and stress management. In the present study, we have performed identificati...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-018-4792-9 |
| _version_ | 1818267115747016704 |
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| author | Mehak Taneja Santosh Kumar Upadhyay |
| author_facet | Mehak Taneja Santosh Kumar Upadhyay |
| author_sort | Mehak Taneja |
| collection | DOAJ |
| description | Abstract Background Plant P-type II Ca2+ATPases are formed by two distinct groups of proteins (ACAs and ECAs) that perform pumping of Ca2+ outside the cytoplasm during homeostasis, and play vital functions during development and stress management. In the present study, we have performed identification and characterisation of P-type II Ca 2+ ATPase gene family in an important crop plant Triticum aestivum. Results Herein, a total of 33 TaACA and 9 TaECA proteins were identified from the various chromosomes and sub-genomes of Triticum aestivum. Phylogenetic analysis revealed clustering of the homoeologous TaACA and TaECA proteins into 11 and 3 distinct groups that exhibited high sequence homology and comparable structural organization as well. Both TaACA and TaECA group proteins consisted of eight to ten transmembrane regions, and their respective domains and motifs. Prediction of sub-cellular localization was found variable for most of the proteins; moreover, it was consistent with the evolutionarily related proteins from rice and Arabidopsis in certain cases. The occurrence of assorted sets of cis-regulatory elements indicated their diverse functions. The differential expression of various TaACA and TaECA genes during developmental stages suggested their roles in growth and development. The modulated expression during heat, drought, salt and biotic stresses along with the occurrence of various stress specific cis-regulatory elements suggested their association with stress response. Interaction of these genes with numerous development and stress related genes indicated their decisive role in various biological processes and signaling. Conclusion T. aestivum genome consisted of a maximum of 42 P-type II Ca 2+ ATPase genes, derived from each A, B and D sub-genome. These genes may play diverse functions during plant growth and development. They may also be involved in signalling during abiotic and biotic stresses. The present study provides a comprehensive insight into the role of P-type II Ca 2+ ATPase genes in T. aestivum. However, the specific function of each gene needs to be established, which could be utilized in future crop improvement programs. |
| first_indexed | 2024-12-12T20:17:28Z |
| format | Article |
| id | doaj.art-d9bd40f0de904069961e29e6ee12c602 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-12T20:17:28Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-d9bd40f0de904069961e29e6ee12c6022022-12-22T00:13:20ZengBMCBMC Genomics1471-21642018-05-0119111610.1186/s12864-018-4792-9Molecular characterization and differential expression suggested diverse functions of P-type II Ca2+ATPases in Triticum aestivum LMehak Taneja0Santosh Kumar Upadhyay1Department of Botany, Panjab UniversityDepartment of Botany, Panjab UniversityAbstract Background Plant P-type II Ca2+ATPases are formed by two distinct groups of proteins (ACAs and ECAs) that perform pumping of Ca2+ outside the cytoplasm during homeostasis, and play vital functions during development and stress management. In the present study, we have performed identification and characterisation of P-type II Ca 2+ ATPase gene family in an important crop plant Triticum aestivum. Results Herein, a total of 33 TaACA and 9 TaECA proteins were identified from the various chromosomes and sub-genomes of Triticum aestivum. Phylogenetic analysis revealed clustering of the homoeologous TaACA and TaECA proteins into 11 and 3 distinct groups that exhibited high sequence homology and comparable structural organization as well. Both TaACA and TaECA group proteins consisted of eight to ten transmembrane regions, and their respective domains and motifs. Prediction of sub-cellular localization was found variable for most of the proteins; moreover, it was consistent with the evolutionarily related proteins from rice and Arabidopsis in certain cases. The occurrence of assorted sets of cis-regulatory elements indicated their diverse functions. The differential expression of various TaACA and TaECA genes during developmental stages suggested their roles in growth and development. The modulated expression during heat, drought, salt and biotic stresses along with the occurrence of various stress specific cis-regulatory elements suggested their association with stress response. Interaction of these genes with numerous development and stress related genes indicated their decisive role in various biological processes and signaling. Conclusion T. aestivum genome consisted of a maximum of 42 P-type II Ca 2+ ATPase genes, derived from each A, B and D sub-genome. These genes may play diverse functions during plant growth and development. They may also be involved in signalling during abiotic and biotic stresses. The present study provides a comprehensive insight into the role of P-type II Ca 2+ ATPase genes in T. aestivum. However, the specific function of each gene needs to be established, which could be utilized in future crop improvement programs.http://link.springer.com/article/10.1186/s12864-018-4792-9Triticum aestivumCa2+ATPasesACAsECAsHeatDrought |
| spellingShingle | Mehak Taneja Santosh Kumar Upadhyay Molecular characterization and differential expression suggested diverse functions of P-type II Ca2+ATPases in Triticum aestivum L BMC Genomics Triticum aestivum Ca2+ATPases ACAs ECAs Heat Drought |
| title | Molecular characterization and differential expression suggested diverse functions of P-type II Ca2+ATPases in Triticum aestivum L |
| title_full | Molecular characterization and differential expression suggested diverse functions of P-type II Ca2+ATPases in Triticum aestivum L |
| title_fullStr | Molecular characterization and differential expression suggested diverse functions of P-type II Ca2+ATPases in Triticum aestivum L |
| title_full_unstemmed | Molecular characterization and differential expression suggested diverse functions of P-type II Ca2+ATPases in Triticum aestivum L |
| title_short | Molecular characterization and differential expression suggested diverse functions of P-type II Ca2+ATPases in Triticum aestivum L |
| title_sort | molecular characterization and differential expression suggested diverse functions of p type ii ca2 atpases in triticum aestivum l |
| topic | Triticum aestivum Ca2+ATPases ACAs ECAs Heat Drought |
| url | http://link.springer.com/article/10.1186/s12864-018-4792-9 |
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